TIM‐3 as a potential exhaustion marker in CD4+ T cells of COVID‐19 patients

Abstract Background COVID‐19 causes a range of clinical symptoms from mild to critical and can be life‐threatening. Up to now, it has led to many deaths. We aimed to evaluate exhausted markers on CD4+ T cells of COVID‐19 patients. Methods In this study, we evaluated 44 patients with confirmed COVID‐...

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Autores principales:	Zahra Modabber, Mehdi Shahbazi, Roghayeh Akbari, Mojgan Bagherzadeh, Alireza Firouzjahi, Mousa Mohammadnia‐Afrouzi
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	CD39 COVID‐19 exhausted Cell PD‐1 TIM‐3 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/3f731d76b8a84fffb1b6af5e008ef3bf
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spelling	oai:doaj.org-article:3f731d76b8a84fffb1b6af5e008ef3bf2021-11-12T19:57:15ZTIM‐3 as a potential exhaustion marker in CD4+ T cells of COVID‐19 patients2050-452710.1002/iid3.526https://doaj.org/article/3f731d76b8a84fffb1b6af5e008ef3bf2021-12-01T00:00:00Zhttps://doi.org/10.1002/iid3.526https://doaj.org/toc/2050-4527Abstract Background COVID‐19 causes a range of clinical symptoms from mild to critical and can be life‐threatening. Up to now, it has led to many deaths. We aimed to evaluate exhausted markers on CD4+ T cells of COVID‐19 patients. Methods In this study, we evaluated 44 patients with confirmed COVID‐19 disease and 16 healthy individuals. Patients were divided into moderate/severe and critical groups. Peripheral blood mononuclear cells (PBMCs) were isolated and stained by anti‐human CD39, PD‐1, TIM‐3, and anti‐human CD4. The percentage of each CD4+ subpopulation was calculated by flow cytometry. Furthermore, we collected clinical information and laboratory data of both control and patient groups. Results We detected overexpression of TIM‐3 on CD4+ T cells in both critical and moderate/severe patients than in healthy individuals (HIs; p < .01 and p < .0001, respectively). CD4+ TIM‐3+ CD39+ lymphocytes were significantly higher in the critical patients than in HI (p < .05). Both Patient groups showed lymphopenia in comparison with HI, but CD4+ lymphocytes did not show any significant difference between study subjects. The increased amount of C‐reactive protein, erythrocyte sedimentation rate, creatinine, blood urea nitrogen, and neutrophil count was observed in patients compared to HI. Conclusion T cell exhaustion occurs during COVID‐19 disease and TIM‐3 is the most important exhausted marker on CD4+ T cells.Zahra ModabberMehdi ShahbaziRoghayeh AkbariMojgan BagherzadehAlireza FirouzjahiMousa Mohammadnia‐AfrouziWileyarticleCD39COVID‐19exhausted CellPD‐1TIM‐3Immunologic diseases. AllergyRC581-607ENImmunity, Inflammation and Disease, Vol 9, Iss 4, Pp 1707-1715 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CD39 COVID‐19 exhausted Cell PD‐1 TIM‐3 Immunologic diseases. Allergy RC581-607
spellingShingle	CD39 COVID‐19 exhausted Cell PD‐1 TIM‐3 Immunologic diseases. Allergy RC581-607 Zahra Modabber Mehdi Shahbazi Roghayeh Akbari Mojgan Bagherzadeh Alireza Firouzjahi Mousa Mohammadnia‐Afrouzi TIM‐3 as a potential exhaustion marker in CD4+ T cells of COVID‐19 patients
description	Abstract Background COVID‐19 causes a range of clinical symptoms from mild to critical and can be life‐threatening. Up to now, it has led to many deaths. We aimed to evaluate exhausted markers on CD4+ T cells of COVID‐19 patients. Methods In this study, we evaluated 44 patients with confirmed COVID‐19 disease and 16 healthy individuals. Patients were divided into moderate/severe and critical groups. Peripheral blood mononuclear cells (PBMCs) were isolated and stained by anti‐human CD39, PD‐1, TIM‐3, and anti‐human CD4. The percentage of each CD4+ subpopulation was calculated by flow cytometry. Furthermore, we collected clinical information and laboratory data of both control and patient groups. Results We detected overexpression of TIM‐3 on CD4+ T cells in both critical and moderate/severe patients than in healthy individuals (HIs; p < .01 and p < .0001, respectively). CD4+ TIM‐3+ CD39+ lymphocytes were significantly higher in the critical patients than in HI (p < .05). Both Patient groups showed lymphopenia in comparison with HI, but CD4+ lymphocytes did not show any significant difference between study subjects. The increased amount of C‐reactive protein, erythrocyte sedimentation rate, creatinine, blood urea nitrogen, and neutrophil count was observed in patients compared to HI. Conclusion T cell exhaustion occurs during COVID‐19 disease and TIM‐3 is the most important exhausted marker on CD4+ T cells.
format	article
author	Zahra Modabber Mehdi Shahbazi Roghayeh Akbari Mojgan Bagherzadeh Alireza Firouzjahi Mousa Mohammadnia‐Afrouzi
author_facet	Zahra Modabber Mehdi Shahbazi Roghayeh Akbari Mojgan Bagherzadeh Alireza Firouzjahi Mousa Mohammadnia‐Afrouzi
author_sort	Zahra Modabber
title	TIM‐3 as a potential exhaustion marker in CD4+ T cells of COVID‐19 patients
title_short	TIM‐3 as a potential exhaustion marker in CD4+ T cells of COVID‐19 patients
title_full	TIM‐3 as a potential exhaustion marker in CD4+ T cells of COVID‐19 patients
title_fullStr	TIM‐3 as a potential exhaustion marker in CD4+ T cells of COVID‐19 patients
title_full_unstemmed	TIM‐3 as a potential exhaustion marker in CD4+ T cells of COVID‐19 patients
title_sort	tim‐3 as a potential exhaustion marker in cd4+ t cells of covid‐19 patients
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/3f731d76b8a84fffb1b6af5e008ef3bf
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TIM‐3 as a potential exhaustion marker in CD4+ T cells of COVID‐19 patients

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