Serologic Evidence for the Exposure of Eastern Coyotes (<italic toggle="yes">Canis latrans</italic>) in Pennsylvania to the Tick-Borne Pathogens <named-content content-type="genus-species">Borreliella burgdorferi</named-content> and <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>

ABSTRACT Lyme disease and anaplasmosis are tick-borne bacterial diseases caused by Borreliella and Anaplasma species, respectively. A comprehensive analysis of the exposure of eastern coyotes (Canis latrans) in the northeastern United States to tick-borne pathogens has not been conducted. In this re...

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Autores principales:	Jerilyn R. Izac, Andrew C. Camire, Edward J. A. Schuler, Amanda L. Hatke, Nathaniel S. O’Bier, Lee D. Oliver, Avery Corondi, Olivia C. Plocinski, Russell P. Desmond, Waheeda A. Naimi, Jason A. Carlyon, Kyle R. Van Why, Jennifer Shelly, Richard T. Marconi
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Anaplasma Borrelia Borreliella coyote Ixodes Lyme disease Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/3f735ef574a54cff94b15f4939452f97
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spelling	oai:doaj.org-article:3f735ef574a54cff94b15f4939452f972021-11-15T15:30:51ZSerologic Evidence for the Exposure of Eastern Coyotes (<italic toggle="yes">Canis latrans</italic>) in Pennsylvania to the Tick-Borne Pathogens <named-content content-type="genus-species">Borreliella burgdorferi</named-content> and <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>10.1128/mSphere.00544-202379-5042https://doaj.org/article/3f735ef574a54cff94b15f4939452f972020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00544-20https://doaj.org/toc/2379-5042ABSTRACT Lyme disease and anaplasmosis are tick-borne bacterial diseases caused by Borreliella and Anaplasma species, respectively. A comprehensive analysis of the exposure of eastern coyotes (Canis latrans) in the northeastern United States to tick-borne pathogens has not been conducted. In this report, we assess the serological status of 128 eastern coyotes harvested in Pennsylvania in 2015 and 2017 for antibodies to Borreliella burgdorferi and Anaplasma phagocytophilum. Immunoblot and dot blot approaches were employed to test each plasma sample by using cell lysates and recombinant proteins as detection antigens. The results demonstrate high seropositivity incidences of 64.8% and 72.7% for B. burgdorferi and A. phagocytophilum, respectively. Antibodies to both pathogens were detected in 51.5% of the plasma samples, indicating high potential for coinfection. Antibodies to the B. burgdorferi proteins DbpB, VlsE, DbpA, BBA36, and OspF (BBO39) were detected in 67.2, 63.3, 56.2, 51.6, and 48.4% of the plasma samples, respectively. Antibodies to the A. phagocytophilum P44 and P130 proteins were detected in 72.7 and 60.9% of the plasma samples, respectively. IMPORTANCE The incidence of Lyme disease (Borreliella burgdorferi) and anaplasmosis (Anaplasma phagocytophilum) are increasing in North America and Europe. The causative agents of these debilitating tick-transmitted infections are maintained in nature in an enzootic cycle involving Ixodes ticks and diverse mammals and birds. It has been postulated that predators directly or indirectly influence the dynamics of the enzootic cycle and disease incidence. Here, we demonstrate high seropositivity of eastern coyotes for B. burgdorferi and A. phagocytophilum. As coyotes become established in urban and suburban environments, interactions with humans, companion animals, and urban/suburban wildlife will increase. Knowledge of the pathogens that these highly adaptable predators are exposed to or carry, and their potential to influence or participate in enzootic cycles, is central to efforts to reduce the risk of tick-borne diseases in humans and companion animals.Jerilyn R. IzacAndrew C. CamireEdward J. A. SchulerAmanda L. HatkeNathaniel S. O’BierLee D. OliverAvery CorondiOlivia C. PlocinskiRussell P. DesmondWaheeda A. NaimiJason A. CarlyonKyle R. Van WhyJennifer ShellyRichard T. MarconiAmerican Society for MicrobiologyarticleAnaplasmaBorreliaBorreliellacoyoteIxodesLyme diseaseMicrobiologyQR1-502ENmSphere, Vol 5, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Anaplasma Borrelia Borreliella coyote Ixodes Lyme disease Microbiology QR1-502
spellingShingle	Anaplasma Borrelia Borreliella coyote Ixodes Lyme disease Microbiology QR1-502 Jerilyn R. Izac Andrew C. Camire Edward J. A. Schuler Amanda L. Hatke Nathaniel S. O’Bier Lee D. Oliver Avery Corondi Olivia C. Plocinski Russell P. Desmond Waheeda A. Naimi Jason A. Carlyon Kyle R. Van Why Jennifer Shelly Richard T. Marconi Serologic Evidence for the Exposure of Eastern Coyotes (<italic toggle="yes">Canis latrans</italic>) in Pennsylvania to the Tick-Borne Pathogens <named-content content-type="genus-species">Borreliella burgdorferi</named-content> and <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>
description	ABSTRACT Lyme disease and anaplasmosis are tick-borne bacterial diseases caused by Borreliella and Anaplasma species, respectively. A comprehensive analysis of the exposure of eastern coyotes (Canis latrans) in the northeastern United States to tick-borne pathogens has not been conducted. In this report, we assess the serological status of 128 eastern coyotes harvested in Pennsylvania in 2015 and 2017 for antibodies to Borreliella burgdorferi and Anaplasma phagocytophilum. Immunoblot and dot blot approaches were employed to test each plasma sample by using cell lysates and recombinant proteins as detection antigens. The results demonstrate high seropositivity incidences of 64.8% and 72.7% for B. burgdorferi and A. phagocytophilum, respectively. Antibodies to both pathogens were detected in 51.5% of the plasma samples, indicating high potential for coinfection. Antibodies to the B. burgdorferi proteins DbpB, VlsE, DbpA, BBA36, and OspF (BBO39) were detected in 67.2, 63.3, 56.2, 51.6, and 48.4% of the plasma samples, respectively. Antibodies to the A. phagocytophilum P44 and P130 proteins were detected in 72.7 and 60.9% of the plasma samples, respectively. IMPORTANCE The incidence of Lyme disease (Borreliella burgdorferi) and anaplasmosis (Anaplasma phagocytophilum) are increasing in North America and Europe. The causative agents of these debilitating tick-transmitted infections are maintained in nature in an enzootic cycle involving Ixodes ticks and diverse mammals and birds. It has been postulated that predators directly or indirectly influence the dynamics of the enzootic cycle and disease incidence. Here, we demonstrate high seropositivity of eastern coyotes for B. burgdorferi and A. phagocytophilum. As coyotes become established in urban and suburban environments, interactions with humans, companion animals, and urban/suburban wildlife will increase. Knowledge of the pathogens that these highly adaptable predators are exposed to or carry, and their potential to influence or participate in enzootic cycles, is central to efforts to reduce the risk of tick-borne diseases in humans and companion animals.
format	article
author	Jerilyn R. Izac Andrew C. Camire Edward J. A. Schuler Amanda L. Hatke Nathaniel S. O’Bier Lee D. Oliver Avery Corondi Olivia C. Plocinski Russell P. Desmond Waheeda A. Naimi Jason A. Carlyon Kyle R. Van Why Jennifer Shelly Richard T. Marconi
author_facet	Jerilyn R. Izac Andrew C. Camire Edward J. A. Schuler Amanda L. Hatke Nathaniel S. O’Bier Lee D. Oliver Avery Corondi Olivia C. Plocinski Russell P. Desmond Waheeda A. Naimi Jason A. Carlyon Kyle R. Van Why Jennifer Shelly Richard T. Marconi
author_sort	Jerilyn R. Izac
title	Serologic Evidence for the Exposure of Eastern Coyotes (<italic toggle="yes">Canis latrans</italic>) in Pennsylvania to the Tick-Borne Pathogens <named-content content-type="genus-species">Borreliella burgdorferi</named-content> and <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>
title_short	Serologic Evidence for the Exposure of Eastern Coyotes (<italic toggle="yes">Canis latrans</italic>) in Pennsylvania to the Tick-Borne Pathogens <named-content content-type="genus-species">Borreliella burgdorferi</named-content> and <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>
title_full	Serologic Evidence for the Exposure of Eastern Coyotes (<italic toggle="yes">Canis latrans</italic>) in Pennsylvania to the Tick-Borne Pathogens <named-content content-type="genus-species">Borreliella burgdorferi</named-content> and <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>
title_fullStr	Serologic Evidence for the Exposure of Eastern Coyotes (<italic toggle="yes">Canis latrans</italic>) in Pennsylvania to the Tick-Borne Pathogens <named-content content-type="genus-species">Borreliella burgdorferi</named-content> and <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>
title_full_unstemmed	Serologic Evidence for the Exposure of Eastern Coyotes (<italic toggle="yes">Canis latrans</italic>) in Pennsylvania to the Tick-Borne Pathogens <named-content content-type="genus-species">Borreliella burgdorferi</named-content> and <named-content content-type="genus-species">Anaplasma phagocytophilum</named-content>
title_sort	serologic evidence for the exposure of eastern coyotes (<italic toggle="yes">canis latrans</italic>) in pennsylvania to the tick-borne pathogens <named-content content-type="genus-species">borreliella burgdorferi</named-content> and <named-content content-type="genus-species">anaplasma phagocytophilum</named-content>
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/3f735ef574a54cff94b15f4939452f97
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