Novel duck reovirus exhibits pathogenicity to specific pathogen-free chickens by the subcutaneous route

Abstract To study the pathogenicity of new duck reovirus (NDRV) to chickens, eighty 3-day-old SPF chickens were equally divided into two groups. The experimental group was inoculated with a NDRV challenge strain of 100 μL (10–5.00 ELD50/0.1 mL) by the subcutaneous (s.c.) route, and the control group...

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Autores principales:	Kexiang Yu, Jinfeng Ti, Xiao Lu, Li Pan, Liping Liu, Yuehua Gao, Xiaozhen Guo, Feng Hu, Cunxia Liu, Xiuli Ma, Yufeng Li, Bing Huang, Minxun Song
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f748364e38043cca46586cf7bec1491
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spelling	oai:doaj.org-article:3f748364e38043cca46586cf7bec14912021-12-02T17:50:41ZNovel duck reovirus exhibits pathogenicity to specific pathogen-free chickens by the subcutaneous route10.1038/s41598-021-90979-w2045-2322https://doaj.org/article/3f748364e38043cca46586cf7bec14912021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90979-whttps://doaj.org/toc/2045-2322Abstract To study the pathogenicity of new duck reovirus (NDRV) to chickens, eighty 3-day-old SPF chickens were equally divided into two groups. The experimental group was inoculated with a NDRV challenge strain of 100 μL (10–5.00 ELD50/0.1 mL) by the subcutaneous (s.c.) route, and the control group was inoculated with 100 μL of sterile phosphate-buffered saline (PBS) by the same route. In the experimental group, chickens exhibited introflexion of claws, performing of splits, stunting syndrome, weight loss and death. Gross lesions such as enlargement and yellowish-white focal necroses were observed in the liver and spleen. Microscopic changes were typical including varying degrees of hepatocyte steatosis and necrosis, splenic lymphocyte necrosis, interstitial pneumonia. Viral loads were detected in lung, liver, heart, spleen, duodenum, burse and kidney. The liver and spleen viral loads remained a much higher level and maintained for a longer time, suggesting that these tissues might be the target organs. In summary, NDRV can cause systemic infections and death in chickens, which indicated that chickens may be infected by NDRV in poultry production.Kexiang YuJinfeng TiXiao LuLi PanLiping LiuYuehua GaoXiaozhen GuoFeng HuCunxia LiuXiuli MaYufeng LiBing HuangMinxun SongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kexiang Yu Jinfeng Ti Xiao Lu Li Pan Liping Liu Yuehua Gao Xiaozhen Guo Feng Hu Cunxia Liu Xiuli Ma Yufeng Li Bing Huang Minxun Song Novel duck reovirus exhibits pathogenicity to specific pathogen-free chickens by the subcutaneous route
description	Abstract To study the pathogenicity of new duck reovirus (NDRV) to chickens, eighty 3-day-old SPF chickens were equally divided into two groups. The experimental group was inoculated with a NDRV challenge strain of 100 μL (10–5.00 ELD50/0.1 mL) by the subcutaneous (s.c.) route, and the control group was inoculated with 100 μL of sterile phosphate-buffered saline (PBS) by the same route. In the experimental group, chickens exhibited introflexion of claws, performing of splits, stunting syndrome, weight loss and death. Gross lesions such as enlargement and yellowish-white focal necroses were observed in the liver and spleen. Microscopic changes were typical including varying degrees of hepatocyte steatosis and necrosis, splenic lymphocyte necrosis, interstitial pneumonia. Viral loads were detected in lung, liver, heart, spleen, duodenum, burse and kidney. The liver and spleen viral loads remained a much higher level and maintained for a longer time, suggesting that these tissues might be the target organs. In summary, NDRV can cause systemic infections and death in chickens, which indicated that chickens may be infected by NDRV in poultry production.
format	article
author	Kexiang Yu Jinfeng Ti Xiao Lu Li Pan Liping Liu Yuehua Gao Xiaozhen Guo Feng Hu Cunxia Liu Xiuli Ma Yufeng Li Bing Huang Minxun Song
author_facet	Kexiang Yu Jinfeng Ti Xiao Lu Li Pan Liping Liu Yuehua Gao Xiaozhen Guo Feng Hu Cunxia Liu Xiuli Ma Yufeng Li Bing Huang Minxun Song
author_sort	Kexiang Yu
title	Novel duck reovirus exhibits pathogenicity to specific pathogen-free chickens by the subcutaneous route
title_short	Novel duck reovirus exhibits pathogenicity to specific pathogen-free chickens by the subcutaneous route
title_full	Novel duck reovirus exhibits pathogenicity to specific pathogen-free chickens by the subcutaneous route
title_fullStr	Novel duck reovirus exhibits pathogenicity to specific pathogen-free chickens by the subcutaneous route
title_full_unstemmed	Novel duck reovirus exhibits pathogenicity to specific pathogen-free chickens by the subcutaneous route
title_sort	novel duck reovirus exhibits pathogenicity to specific pathogen-free chickens by the subcutaneous route
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3f748364e38043cca46586cf7bec1491
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Novel duck reovirus exhibits pathogenicity to specific pathogen-free chickens by the subcutaneous route

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