A complex of equine lysozyme and oleic acid with bactericidal activity against Streptococcus pneumoniae.

HAMLET and ELOA are complexes consisting of oleic acid and two homologous, yet functionally different, proteins with cytotoxic activities against mammalian cells, with HAMLET showing higher tumor cells specificity, possibly due to the difference in propensity for oleic acid binding, as HAMLET binds...

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Autores principales:	Emily A Clementi, Kristina R Wilhelm, Jürgen Schleucher, Ludmilla A Morozova-Roche, Anders P Hakansson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f755a21fffc42cebb55afcaaa650a5a
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spelling	oai:doaj.org-article:3f755a21fffc42cebb55afcaaa650a5a2021-11-18T08:46:03ZA complex of equine lysozyme and oleic acid with bactericidal activity against Streptococcus pneumoniae.1932-620310.1371/journal.pone.0080649https://doaj.org/article/3f755a21fffc42cebb55afcaaa650a5a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24260444/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203HAMLET and ELOA are complexes consisting of oleic acid and two homologous, yet functionally different, proteins with cytotoxic activities against mammalian cells, with HAMLET showing higher tumor cells specificity, possibly due to the difference in propensity for oleic acid binding, as HAMLET binds 5-8 oleic acid molecules per protein molecule and ELOA binds 11-48 oleic acids. HAMLET has been shown to possess bactericidal activity against a number of bacterial species, particularly those with a respiratory tropism, with Streptococcus pneumoniae displaying the greatest degree of sensitivity. We show here that ELOA also displays bactericidal activity against pneumococci, which at lower concentrations shows mechanistic similarities to HAMLET's bactericidal activity. ELOA binds to S. pneumoniae and causes perturbations of the plasma membrane, including depolarization and subsequent rupture, and activates an influx of calcium into the cells. Selective inhibition of calcium channels and sodium/calcium exchange activity significantly diminished ELOA's bactericidal activity, similar to what we have observed with HAMLET. Finally, ELOA-induced death was also accompanied by DNA fragmentation into high molecular weight fragments - an apoptosis-like morphological phenotype that is seen during HAMLET-induced death. Thus, in contrast to different mechanisms of eukaryote cell death induced by ELOA and HAMLET, these complexes are characterized by rather similar activities towards bacteria. Although the majority of these events could be mimicked using oleic acid alone, the concentrations of oleic acid required were significantly higher than those present in the ELOA complex, and for some assays, the results were not identical between oleic acid alone and the ELOA complex. This indicates that the lipid, as a common denominator in both complexes, is an important component for the complexes' bactericidal activities, while the proteins are required both to solubilize and/or present the lipid at the bacterial membrane and likely to confer other and separate functions during the bacterial death.Emily A ClementiKristina R WilhelmJürgen SchleucherLudmilla A Morozova-RocheAnders P HakanssonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e80649 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emily A Clementi Kristina R Wilhelm Jürgen Schleucher Ludmilla A Morozova-Roche Anders P Hakansson A complex of equine lysozyme and oleic acid with bactericidal activity against Streptococcus pneumoniae.
description	HAMLET and ELOA are complexes consisting of oleic acid and two homologous, yet functionally different, proteins with cytotoxic activities against mammalian cells, with HAMLET showing higher tumor cells specificity, possibly due to the difference in propensity for oleic acid binding, as HAMLET binds 5-8 oleic acid molecules per protein molecule and ELOA binds 11-48 oleic acids. HAMLET has been shown to possess bactericidal activity against a number of bacterial species, particularly those with a respiratory tropism, with Streptococcus pneumoniae displaying the greatest degree of sensitivity. We show here that ELOA also displays bactericidal activity against pneumococci, which at lower concentrations shows mechanistic similarities to HAMLET's bactericidal activity. ELOA binds to S. pneumoniae and causes perturbations of the plasma membrane, including depolarization and subsequent rupture, and activates an influx of calcium into the cells. Selective inhibition of calcium channels and sodium/calcium exchange activity significantly diminished ELOA's bactericidal activity, similar to what we have observed with HAMLET. Finally, ELOA-induced death was also accompanied by DNA fragmentation into high molecular weight fragments - an apoptosis-like morphological phenotype that is seen during HAMLET-induced death. Thus, in contrast to different mechanisms of eukaryote cell death induced by ELOA and HAMLET, these complexes are characterized by rather similar activities towards bacteria. Although the majority of these events could be mimicked using oleic acid alone, the concentrations of oleic acid required were significantly higher than those present in the ELOA complex, and for some assays, the results were not identical between oleic acid alone and the ELOA complex. This indicates that the lipid, as a common denominator in both complexes, is an important component for the complexes' bactericidal activities, while the proteins are required both to solubilize and/or present the lipid at the bacterial membrane and likely to confer other and separate functions during the bacterial death.
format	article
author	Emily A Clementi Kristina R Wilhelm Jürgen Schleucher Ludmilla A Morozova-Roche Anders P Hakansson
author_facet	Emily A Clementi Kristina R Wilhelm Jürgen Schleucher Ludmilla A Morozova-Roche Anders P Hakansson
author_sort	Emily A Clementi
title	A complex of equine lysozyme and oleic acid with bactericidal activity against Streptococcus pneumoniae.
title_short	A complex of equine lysozyme and oleic acid with bactericidal activity against Streptococcus pneumoniae.
title_full	A complex of equine lysozyme and oleic acid with bactericidal activity against Streptococcus pneumoniae.
title_fullStr	A complex of equine lysozyme and oleic acid with bactericidal activity against Streptococcus pneumoniae.
title_full_unstemmed	A complex of equine lysozyme and oleic acid with bactericidal activity against Streptococcus pneumoniae.
title_sort	complex of equine lysozyme and oleic acid with bactericidal activity against streptococcus pneumoniae.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3f755a21fffc42cebb55afcaaa650a5a
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A complex of equine lysozyme and oleic acid with bactericidal activity against Streptococcus pneumoniae.

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