Molecular model of a sensor of two-component signaling system

Abstract Two-component systems (TCS) are widespread signaling systems present in all domains of life. TCS typically consist of a signal receptor/transducer and a response regulator. The receptors (histidine kinases, chemoreceptors and photoreceptors) are often embedded in the membrane and have a sim...

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Autores principales:	Yury L. Ryzhykau, Philipp S. Orekhov, Maksim I. Rulev, Alexey V. Vlasov, Igor A. Melnikov, Dmytro A. Volkov, Mikhail Yu. Nikolaev, Dmitrii V. Zabelskii, Tatiana N. Murugova, Vladimir V. Chupin, Andrey V. Rogachev, Andrey Yu. Gruzinov, Dmitri I. Svergun, Martha E. Brennich, Ivan Yu. Gushchin, Montserrat Soler-Lopez, Arne Bothe, Georg Büldt, Gordon Leonard, Martin Engelhard, Alexander I. Kuklin, Valentin I. Gordeliy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f760df645e345b1a2dd8988f064b12e
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spelling	oai:doaj.org-article:3f760df645e345b1a2dd8988f064b12e2021-12-02T15:49:53ZMolecular model of a sensor of two-component signaling system10.1038/s41598-021-89613-62045-2322https://doaj.org/article/3f760df645e345b1a2dd8988f064b12e2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89613-6https://doaj.org/toc/2045-2322Abstract Two-component systems (TCS) are widespread signaling systems present in all domains of life. TCS typically consist of a signal receptor/transducer and a response regulator. The receptors (histidine kinases, chemoreceptors and photoreceptors) are often embedded in the membrane and have a similar modular structure. Chemoreceptors were shown to function in highly ordered arrays, with trimers of dimers being the smallest functional unit. However, much less is known about photoreceptors. Here, we use small-angle scattering (SAS) to show that detergent-solubilized sensory rhodopsin II in complex with its cognate transducer forms dimers at low salt concentration, which associate into trimers of dimers at higher buffer molarities. We then fit an atomistic model of the whole complex into the SAS data. The obtained results suggest that the trimer of dimers is "tripod"-shaped and that the contacts between the dimers occur only through their cytoplasmic regions, whereas the transmembrane regions remain unconnected.Yury L. RyzhykauPhilipp S. OrekhovMaksim I. RulevAlexey V. VlasovIgor A. MelnikovDmytro A. VolkovMikhail Yu. NikolaevDmitrii V. ZabelskiiTatiana N. MurugovaVladimir V. ChupinAndrey V. RogachevAndrey Yu. GruzinovDmitri I. SvergunMartha E. BrennichIvan Yu. GushchinMontserrat Soler-LopezArne BotheGeorg BüldtGordon LeonardMartin EngelhardAlexander I. KuklinValentin I. GordeliyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yury L. Ryzhykau Philipp S. Orekhov Maksim I. Rulev Alexey V. Vlasov Igor A. Melnikov Dmytro A. Volkov Mikhail Yu. Nikolaev Dmitrii V. Zabelskii Tatiana N. Murugova Vladimir V. Chupin Andrey V. Rogachev Andrey Yu. Gruzinov Dmitri I. Svergun Martha E. Brennich Ivan Yu. Gushchin Montserrat Soler-Lopez Arne Bothe Georg Büldt Gordon Leonard Martin Engelhard Alexander I. Kuklin Valentin I. Gordeliy Molecular model of a sensor of two-component signaling system
description	Abstract Two-component systems (TCS) are widespread signaling systems present in all domains of life. TCS typically consist of a signal receptor/transducer and a response regulator. The receptors (histidine kinases, chemoreceptors and photoreceptors) are often embedded in the membrane and have a similar modular structure. Chemoreceptors were shown to function in highly ordered arrays, with trimers of dimers being the smallest functional unit. However, much less is known about photoreceptors. Here, we use small-angle scattering (SAS) to show that detergent-solubilized sensory rhodopsin II in complex with its cognate transducer forms dimers at low salt concentration, which associate into trimers of dimers at higher buffer molarities. We then fit an atomistic model of the whole complex into the SAS data. The obtained results suggest that the trimer of dimers is "tripod"-shaped and that the contacts between the dimers occur only through their cytoplasmic regions, whereas the transmembrane regions remain unconnected.
format	article
author	Yury L. Ryzhykau Philipp S. Orekhov Maksim I. Rulev Alexey V. Vlasov Igor A. Melnikov Dmytro A. Volkov Mikhail Yu. Nikolaev Dmitrii V. Zabelskii Tatiana N. Murugova Vladimir V. Chupin Andrey V. Rogachev Andrey Yu. Gruzinov Dmitri I. Svergun Martha E. Brennich Ivan Yu. Gushchin Montserrat Soler-Lopez Arne Bothe Georg Büldt Gordon Leonard Martin Engelhard Alexander I. Kuklin Valentin I. Gordeliy
author_facet	Yury L. Ryzhykau Philipp S. Orekhov Maksim I. Rulev Alexey V. Vlasov Igor A. Melnikov Dmytro A. Volkov Mikhail Yu. Nikolaev Dmitrii V. Zabelskii Tatiana N. Murugova Vladimir V. Chupin Andrey V. Rogachev Andrey Yu. Gruzinov Dmitri I. Svergun Martha E. Brennich Ivan Yu. Gushchin Montserrat Soler-Lopez Arne Bothe Georg Büldt Gordon Leonard Martin Engelhard Alexander I. Kuklin Valentin I. Gordeliy
author_sort	Yury L. Ryzhykau
title	Molecular model of a sensor of two-component signaling system
title_short	Molecular model of a sensor of two-component signaling system
title_full	Molecular model of a sensor of two-component signaling system
title_fullStr	Molecular model of a sensor of two-component signaling system
title_full_unstemmed	Molecular model of a sensor of two-component signaling system
title_sort	molecular model of a sensor of two-component signaling system
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3f760df645e345b1a2dd8988f064b12e
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Molecular model of a sensor of two-component signaling system

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