NF-κB plays a key role in inducing CD274 expression in human monocytes after lipopolysaccharide treatment.

CD274, one of two co-stimulatory ligands for programmed death 1 and widely expressed in the mononuclear phagocyte system (MPS), may co-stimulate T cells and regulates inflammatory responses. However, changes in CD274 gene expression and the underlying molecular mechanism are poorly understood during...

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Autores principales:	Gang Huang, Qianjun Wen, Yongliang Zhao, Qiangguo Gao, Yun Bai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f7809abbfe54d8ea80fac8e8f84769e
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spelling	oai:doaj.org-article:3f7809abbfe54d8ea80fac8e8f84769e2021-11-18T07:49:56ZNF-κB plays a key role in inducing CD274 expression in human monocytes after lipopolysaccharide treatment.1932-620310.1371/journal.pone.0061602https://doaj.org/article/3f7809abbfe54d8ea80fac8e8f84769e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23585913/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203CD274, one of two co-stimulatory ligands for programmed death 1 and widely expressed in the mononuclear phagocyte system (MPS), may co-stimulate T cells and regulates inflammatory responses. However, changes in CD274 gene expression and the underlying molecular mechanism are poorly understood during inflammatory responses. Therefore, delineation of the complex mechanisms regulating CD274 expression is critical to understand this immunoregulatory system during inflammatory responses. The purpose of this study was to assess the molecular mechanisms regulating CD274 expression in an in vitro monocyte model of inflammatory response. Firstly, CD274 expression levels in human primary monocytes after lipopolysaccharide (LPS) treatment were observed and correlated with NF-κB activation. Secondly, based on the distribution of putative NF-κB binding sites, 5' truncated human CD274 promoter reporters were constructed, transfected into U937 cells and critical promoter regions for basal (nt -570 to +94) and LPS-induced (nt -1735 to -570) transcription were identified by dual luciferase assays. Finally, a key NF-κB binding site (nt -610 to -601) for LPS-inducible CD274 transcriptional activity was characterized by point mutation analysis and chromatin immunoprecipitation analysis assays (ChIP). Thus, the present study establishes a molecular basis to understand the mechanisms governing CD274 expression in certain infections and inflammatory disorders.Gang HuangQianjun WenYongliang ZhaoQiangguo GaoYun BaiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e61602 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gang Huang Qianjun Wen Yongliang Zhao Qiangguo Gao Yun Bai NF-κB plays a key role in inducing CD274 expression in human monocytes after lipopolysaccharide treatment.
description	CD274, one of two co-stimulatory ligands for programmed death 1 and widely expressed in the mononuclear phagocyte system (MPS), may co-stimulate T cells and regulates inflammatory responses. However, changes in CD274 gene expression and the underlying molecular mechanism are poorly understood during inflammatory responses. Therefore, delineation of the complex mechanisms regulating CD274 expression is critical to understand this immunoregulatory system during inflammatory responses. The purpose of this study was to assess the molecular mechanisms regulating CD274 expression in an in vitro monocyte model of inflammatory response. Firstly, CD274 expression levels in human primary monocytes after lipopolysaccharide (LPS) treatment were observed and correlated with NF-κB activation. Secondly, based on the distribution of putative NF-κB binding sites, 5' truncated human CD274 promoter reporters were constructed, transfected into U937 cells and critical promoter regions for basal (nt -570 to +94) and LPS-induced (nt -1735 to -570) transcription were identified by dual luciferase assays. Finally, a key NF-κB binding site (nt -610 to -601) for LPS-inducible CD274 transcriptional activity was characterized by point mutation analysis and chromatin immunoprecipitation analysis assays (ChIP). Thus, the present study establishes a molecular basis to understand the mechanisms governing CD274 expression in certain infections and inflammatory disorders.
format	article
author	Gang Huang Qianjun Wen Yongliang Zhao Qiangguo Gao Yun Bai
author_facet	Gang Huang Qianjun Wen Yongliang Zhao Qiangguo Gao Yun Bai
author_sort	Gang Huang
title	NF-κB plays a key role in inducing CD274 expression in human monocytes after lipopolysaccharide treatment.
title_short	NF-κB plays a key role in inducing CD274 expression in human monocytes after lipopolysaccharide treatment.
title_full	NF-κB plays a key role in inducing CD274 expression in human monocytes after lipopolysaccharide treatment.
title_fullStr	NF-κB plays a key role in inducing CD274 expression in human monocytes after lipopolysaccharide treatment.
title_full_unstemmed	NF-κB plays a key role in inducing CD274 expression in human monocytes after lipopolysaccharide treatment.
title_sort	nf-κb plays a key role in inducing cd274 expression in human monocytes after lipopolysaccharide treatment.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3f7809abbfe54d8ea80fac8e8f84769e
work_keys_str_mv	AT ganghuang nfkbplaysakeyroleininducingcd274expressioninhumanmonocytesafterlipopolysaccharidetreatment AT qianjunwen nfkbplaysakeyroleininducingcd274expressioninhumanmonocytesafterlipopolysaccharidetreatment AT yongliangzhao nfkbplaysakeyroleininducingcd274expressioninhumanmonocytesafterlipopolysaccharidetreatment AT qiangguogao nfkbplaysakeyroleininducingcd274expressioninhumanmonocytesafterlipopolysaccharidetreatment AT yunbai nfkbplaysakeyroleininducingcd274expressioninhumanmonocytesafterlipopolysaccharidetreatment
_version_	1718422905784631296

NF-κB plays a key role in inducing CD274 expression in human monocytes after lipopolysaccharide treatment.

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