Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement

Abstract Serum is a stable medium supplement for in vitro cell culture. Live cells are used in stem cell research, drug toxicity and safety testing, disease diagnosis and prevention, and development of antibiotics, drugs, and vaccines. However, use of serum in culture involves concerns such as an et...

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Autores principales:	Younsik Jeong, Woon-Yong Choi, Areumi Park, Yeon-Ji Lee, Youngdeuk Lee, Gun-Hoo Park, Su-Jin Lee, Won-Kyu Lee, Yong-Kyun Ryu, Do-Hyung Kang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f7bd6ee6b99483abd7396ab0f887993
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spelling	oai:doaj.org-article:3f7bd6ee6b99483abd7396ab0f8879932021-12-02T11:37:18ZMarine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement10.1038/s41598-021-84558-22045-2322https://doaj.org/article/3f7bd6ee6b99483abd7396ab0f8879932021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84558-2https://doaj.org/toc/2045-2322Abstract Serum is a stable medium supplement for in vitro cell culture. Live cells are used in stem cell research, drug toxicity and safety testing, disease diagnosis and prevention, and development of antibiotics, drugs, and vaccines. However, use of serum in culture involves concerns such as an ethical debate regarding the collection process, lack of standardized ingredients, and high cost. Herein, therefore, we evaluated the possibility of using edible cyanobacterium (Spirulina maxima), which is a nutrient-rich, sustainable, and ethically acceptable source, as a novel substitute for fetal bovine serum (FBS). H460 cells were cultured to the 10th generation by adding a mixture of spirulina animal cell culture solution (SACCS) and FBS to the culture medium. Cell morphology and viability, cell cycle, apoptosis, proteomes, and transcriptomes were assessed. We observed that SACCS had better growth-promoting capabilities than FBS. Cell proliferation was promoted even when FBS was replaced by 50–70% SACCS; there was no significant difference in cell shape or viability. There were only slight differences in the cell cycle, apoptosis, proteomes, and transcriptomes of the cells grown in presence of SACCS. Therefore, SACCS has the potential to be an effective, low-cost, and eco-friendly alternative to FBS in in vitro culture.Younsik JeongWoon-Yong ChoiAreumi ParkYeon-Ji LeeYoungdeuk LeeGun-Hoo ParkSu-Jin LeeWon-Kyu LeeYong-Kyun RyuDo-Hyung KangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Younsik Jeong Woon-Yong Choi Areumi Park Yeon-Ji Lee Youngdeuk Lee Gun-Hoo Park Su-Jin Lee Won-Kyu Lee Yong-Kyun Ryu Do-Hyung Kang Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement
description	Abstract Serum is a stable medium supplement for in vitro cell culture. Live cells are used in stem cell research, drug toxicity and safety testing, disease diagnosis and prevention, and development of antibiotics, drugs, and vaccines. However, use of serum in culture involves concerns such as an ethical debate regarding the collection process, lack of standardized ingredients, and high cost. Herein, therefore, we evaluated the possibility of using edible cyanobacterium (Spirulina maxima), which is a nutrient-rich, sustainable, and ethically acceptable source, as a novel substitute for fetal bovine serum (FBS). H460 cells were cultured to the 10th generation by adding a mixture of spirulina animal cell culture solution (SACCS) and FBS to the culture medium. Cell morphology and viability, cell cycle, apoptosis, proteomes, and transcriptomes were assessed. We observed that SACCS had better growth-promoting capabilities than FBS. Cell proliferation was promoted even when FBS was replaced by 50–70% SACCS; there was no significant difference in cell shape or viability. There were only slight differences in the cell cycle, apoptosis, proteomes, and transcriptomes of the cells grown in presence of SACCS. Therefore, SACCS has the potential to be an effective, low-cost, and eco-friendly alternative to FBS in in vitro culture.
format	article
author	Younsik Jeong Woon-Yong Choi Areumi Park Yeon-Ji Lee Youngdeuk Lee Gun-Hoo Park Su-Jin Lee Won-Kyu Lee Yong-Kyun Ryu Do-Hyung Kang
author_facet	Younsik Jeong Woon-Yong Choi Areumi Park Yeon-Ji Lee Youngdeuk Lee Gun-Hoo Park Su-Jin Lee Won-Kyu Lee Yong-Kyun Ryu Do-Hyung Kang
author_sort	Younsik Jeong
title	Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement
title_short	Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement
title_full	Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement
title_fullStr	Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement
title_full_unstemmed	Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement
title_sort	marine cyanobacterium spirulina maxima as an alternate to the animal cell culture medium supplement
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3f7bd6ee6b99483abd7396ab0f887993
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Marine cyanobacterium Spirulina maxima as an alternate to the animal cell culture medium supplement

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