Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response

Abstract Circulating cell-free DNA (cfDNA) may be involved in immune response regulation. We studied the variations in abundance of telomeric sequences in plasma and serum in young healthy volunteers and the ability of cfDNA contained in these samples to co-activate the TNF-α m RNA expression in mon...

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Autores principales:	Alzbeta Zinkova, Iva Brynychova, Alexander Svacina, Marie Jirkovska, Marie Korabecna
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f7efc2cfe4c4bbd97479939d0bca88f
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spelling	oai:doaj.org-article:3f7efc2cfe4c4bbd97479939d0bca88f2021-12-02T16:07:45ZCell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response10.1038/s41598-017-02905-82045-2322https://doaj.org/article/3f7efc2cfe4c4bbd97479939d0bca88f2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02905-8https://doaj.org/toc/2045-2322Abstract Circulating cell-free DNA (cfDNA) may be involved in immune response regulation. We studied the variations in abundance of telomeric sequences in plasma and serum in young healthy volunteers and the ability of cfDNA contained in these samples to co-activate the TNF-α m RNA expression in monocytes. We performed qPCR to determine relative telomere length (T/S ratios) in plasma, serum and whole blood of 36 volunteers. Using paired samples of plasma and serum and DNase treatment, we analysed the contribution of cfDNA to the co-activation of TNF-α mRNA expression in THP1 monocytic cell line. We found significant differences between paired plasma and serum samples in relative T/S ratios (median 1.38 ± 1.1 vs. 0.86 ± 0.25, respectively) and in total amounts of cfDNA and in estimated total amounts of telomeres which were significantly higher in serum than in plasma. TNF-α mRNA expression in THP1 cells increased significantly after DNase treatment of all samples used for stimulation. The highest TNF-α mRNA expressions were observed after stimulation with DNase treated serum samples. Our results suggest that the different content of telomeric sequences in plasma and serum may contribute to the tuning of immune response. Further studies of this interesting phenomenon are needed.Alzbeta ZinkovaIva BrynychovaAlexander SvacinaMarie JirkovskaMarie KorabecnaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alzbeta Zinkova Iva Brynychova Alexander Svacina Marie Jirkovska Marie Korabecna Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
description	Abstract Circulating cell-free DNA (cfDNA) may be involved in immune response regulation. We studied the variations in abundance of telomeric sequences in plasma and serum in young healthy volunteers and the ability of cfDNA contained in these samples to co-activate the TNF-α m RNA expression in monocytes. We performed qPCR to determine relative telomere length (T/S ratios) in plasma, serum and whole blood of 36 volunteers. Using paired samples of plasma and serum and DNase treatment, we analysed the contribution of cfDNA to the co-activation of TNF-α mRNA expression in THP1 monocytic cell line. We found significant differences between paired plasma and serum samples in relative T/S ratios (median 1.38 ± 1.1 vs. 0.86 ± 0.25, respectively) and in total amounts of cfDNA and in estimated total amounts of telomeres which were significantly higher in serum than in plasma. TNF-α mRNA expression in THP1 cells increased significantly after DNase treatment of all samples used for stimulation. The highest TNF-α mRNA expressions were observed after stimulation with DNase treated serum samples. Our results suggest that the different content of telomeric sequences in plasma and serum may contribute to the tuning of immune response. Further studies of this interesting phenomenon are needed.
format	article
author	Alzbeta Zinkova Iva Brynychova Alexander Svacina Marie Jirkovska Marie Korabecna
author_facet	Alzbeta Zinkova Iva Brynychova Alexander Svacina Marie Jirkovska Marie Korabecna
author_sort	Alzbeta Zinkova
title	Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
title_short	Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
title_full	Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
title_fullStr	Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
title_full_unstemmed	Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
title_sort	cell-free dna from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3f7efc2cfe4c4bbd97479939d0bca88f
work_keys_str_mv	AT alzbetazinkova cellfreednafromhumanplasmaandserumdiffersincontentoftelomericsequencesanditsabilitytopromoteimmuneresponse AT ivabrynychova cellfreednafromhumanplasmaandserumdiffersincontentoftelomericsequencesanditsabilitytopromoteimmuneresponse AT alexandersvacina cellfreednafromhumanplasmaandserumdiffersincontentoftelomericsequencesanditsabilitytopromoteimmuneresponse AT mariejirkovska cellfreednafromhumanplasmaandserumdiffersincontentoftelomericsequencesanditsabilitytopromoteimmuneresponse AT mariekorabecna cellfreednafromhumanplasmaandserumdiffersincontentoftelomericsequencesanditsabilitytopromoteimmuneresponse
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Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response

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