Unraveling the Metabolic Requirements of the Gut Commensal Bacteroides ovatus

Background: Bacteroidetes are the most common bacterial phylum in the mammalian intestine and the effects of several Bacteroides spp. on multiple facets of host physiology have been previously described. Of the Bacteroides spp., Bacteroides ovatus has recently garnered attention due to its beneficia...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Robert Fultz, Taylor Ticer, Faith D. Ihekweazu, Thomas D. Horvath, Sigmund J. Haidacher, Kathleen M. Hoch, Meghna Bajaj, Jennifer K. Spinler, Anthony M. Haag, Shelly A. Buffington, Melinda A. Engevik
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Bacteroides metabolism carbohydrates polysaccharides intestine commensal Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/3f81ecd7e8bc48e2bb3e0901f01bfe38
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3f81ecd7e8bc48e2bb3e0901f01bfe38
record_format	dspace
spelling	oai:doaj.org-article:3f81ecd7e8bc48e2bb3e0901f01bfe382021-12-01T01:26:33ZUnraveling the Metabolic Requirements of the Gut Commensal Bacteroides ovatus1664-302X10.3389/fmicb.2021.745469https://doaj.org/article/3f81ecd7e8bc48e2bb3e0901f01bfe382021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.745469/fullhttps://doaj.org/toc/1664-302XBackground: Bacteroidetes are the most common bacterial phylum in the mammalian intestine and the effects of several Bacteroides spp. on multiple facets of host physiology have been previously described. Of the Bacteroides spp., Bacteroides ovatus has recently garnered attention due to its beneficial effects in the context of intestinal inflammation. In this study, we aimed to examine model host intestinal physiological conditions and dietary modifications to characterize their effects on B. ovatus growth.Methods and Results: Using Biolog phenotypic microarrays, we evaluated 62 primary carbon sources and determined that B. ovatus ATCC 8384 can use the following carbohydrates as primary carbon sources: 10 disaccharides, 4 trisaccharides, 4 polysaccharides, 4 polymers, 3 L-linked sugars, 6 D-linked sugars, 5 amino-sugars, 6 alcohol sugars, and 15 organic acids. Proteomic profiling of B. ovatus bacteria revealed that a significant portion of the B. ovatus proteome contains proteins important for metabolism. Among the proteins, we found glycosyl hydrolase (GH) familes GH2, GH5, GH20, GH 43, GH88, GH92, and GH95. We also identified multiple proteins with antioxidant properties and reasoned that these proteins may support B. ovatus growth in the GI tract. Upon further testing, we showed that B. ovatus grew robustly in various pH, osmolarity, bile, ethanol, and H2O2 concentrations; indicating that B. ovatus is a well-adapted gut microbe.Conclusion: Taken together, we have demonstrated that key host and diet-derived changes in the intestinal environment influence B. ovatus growth. These data provide the framework for future work toward understanding how diet and lifestyle interventions may promote a beneficial environment for B. ovatus growth.Robert FultzTaylor TicerFaith D. IhekweazuFaith D. IhekweazuThomas D. HorvathThomas D. HorvathSigmund J. HaidacherSigmund J. HaidacherKathleen M. HochKathleen M. HochMeghna BajajJennifer K. SpinlerJennifer K. SpinlerAnthony M. HaagAnthony M. HaagShelly A. BuffingtonMelinda A. EngevikFrontiers Media S.A.articleBacteroidesmetabolismcarbohydratespolysaccharidesintestinecommensalMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bacteroides metabolism carbohydrates polysaccharides intestine commensal Microbiology QR1-502
spellingShingle	Bacteroides metabolism carbohydrates polysaccharides intestine commensal Microbiology QR1-502 Robert Fultz Taylor Ticer Faith D. Ihekweazu Faith D. Ihekweazu Thomas D. Horvath Thomas D. Horvath Sigmund J. Haidacher Sigmund J. Haidacher Kathleen M. Hoch Kathleen M. Hoch Meghna Bajaj Jennifer K. Spinler Jennifer K. Spinler Anthony M. Haag Anthony M. Haag Shelly A. Buffington Melinda A. Engevik Unraveling the Metabolic Requirements of the Gut Commensal Bacteroides ovatus
description	Background: Bacteroidetes are the most common bacterial phylum in the mammalian intestine and the effects of several Bacteroides spp. on multiple facets of host physiology have been previously described. Of the Bacteroides spp., Bacteroides ovatus has recently garnered attention due to its beneficial effects in the context of intestinal inflammation. In this study, we aimed to examine model host intestinal physiological conditions and dietary modifications to characterize their effects on B. ovatus growth.Methods and Results: Using Biolog phenotypic microarrays, we evaluated 62 primary carbon sources and determined that B. ovatus ATCC 8384 can use the following carbohydrates as primary carbon sources: 10 disaccharides, 4 trisaccharides, 4 polysaccharides, 4 polymers, 3 L-linked sugars, 6 D-linked sugars, 5 amino-sugars, 6 alcohol sugars, and 15 organic acids. Proteomic profiling of B. ovatus bacteria revealed that a significant portion of the B. ovatus proteome contains proteins important for metabolism. Among the proteins, we found glycosyl hydrolase (GH) familes GH2, GH5, GH20, GH 43, GH88, GH92, and GH95. We also identified multiple proteins with antioxidant properties and reasoned that these proteins may support B. ovatus growth in the GI tract. Upon further testing, we showed that B. ovatus grew robustly in various pH, osmolarity, bile, ethanol, and H2O2 concentrations; indicating that B. ovatus is a well-adapted gut microbe.Conclusion: Taken together, we have demonstrated that key host and diet-derived changes in the intestinal environment influence B. ovatus growth. These data provide the framework for future work toward understanding how diet and lifestyle interventions may promote a beneficial environment for B. ovatus growth.
format	article
author	Robert Fultz Taylor Ticer Faith D. Ihekweazu Faith D. Ihekweazu Thomas D. Horvath Thomas D. Horvath Sigmund J. Haidacher Sigmund J. Haidacher Kathleen M. Hoch Kathleen M. Hoch Meghna Bajaj Jennifer K. Spinler Jennifer K. Spinler Anthony M. Haag Anthony M. Haag Shelly A. Buffington Melinda A. Engevik
author_facet	Robert Fultz Taylor Ticer Faith D. Ihekweazu Faith D. Ihekweazu Thomas D. Horvath Thomas D. Horvath Sigmund J. Haidacher Sigmund J. Haidacher Kathleen M. Hoch Kathleen M. Hoch Meghna Bajaj Jennifer K. Spinler Jennifer K. Spinler Anthony M. Haag Anthony M. Haag Shelly A. Buffington Melinda A. Engevik
author_sort	Robert Fultz
title	Unraveling the Metabolic Requirements of the Gut Commensal Bacteroides ovatus
title_short	Unraveling the Metabolic Requirements of the Gut Commensal Bacteroides ovatus
title_full	Unraveling the Metabolic Requirements of the Gut Commensal Bacteroides ovatus
title_fullStr	Unraveling the Metabolic Requirements of the Gut Commensal Bacteroides ovatus
title_full_unstemmed	Unraveling the Metabolic Requirements of the Gut Commensal Bacteroides ovatus
title_sort	unraveling the metabolic requirements of the gut commensal bacteroides ovatus
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3f81ecd7e8bc48e2bb3e0901f01bfe38
work_keys_str_mv	AT robertfultz unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT taylorticer unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT faithdihekweazu unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT faithdihekweazu unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT thomasdhorvath unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT thomasdhorvath unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT sigmundjhaidacher unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT sigmundjhaidacher unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT kathleenmhoch unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT kathleenmhoch unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT meghnabajaj unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT jenniferkspinler unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT jenniferkspinler unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT anthonymhaag unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT anthonymhaag unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT shellyabuffington unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus AT melindaaengevik unravelingthemetabolicrequirementsofthegutcommensalbacteroidesovatus
_version_	1718405954014281728

Unraveling the Metabolic Requirements of the Gut Commensal Bacteroides ovatus

Ejemplares similares