Characterization and diversity of phages infecting Aeromonas salmonicida subsp. salmonicida

Abstract Phages infecting Aeromonas salmonicida subsp. salmonicida, the causative agent of the fish disease furunculosis, have been isolated for decades but very few of them have been characterized. Here, the host range of 12 virulent phages, including three isolated in the present study, was evalua...

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Autores principales:	Antony T. Vincent, Valérie E. Paquet, Alex Bernatchez, Denise M. Tremblay, Sylvain Moineau, Steve J. Charette
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f8563be5ebb439da3f70d2eb119da81
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spelling	oai:doaj.org-article:3f8563be5ebb439da3f70d2eb119da812021-12-02T15:04:54ZCharacterization and diversity of phages infecting Aeromonas salmonicida subsp. salmonicida10.1038/s41598-017-07401-72045-2322https://doaj.org/article/3f8563be5ebb439da3f70d2eb119da812017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07401-7https://doaj.org/toc/2045-2322Abstract Phages infecting Aeromonas salmonicida subsp. salmonicida, the causative agent of the fish disease furunculosis, have been isolated for decades but very few of them have been characterized. Here, the host range of 12 virulent phages, including three isolated in the present study, was evaluated against a panel of 65 A. salmonicida isolates, including representatives of the psychrophilic subspecies salmonicida, smithia, masoucida, and the mesophilic subspecies pectinolytica. This bacterial set also included three isolates from India suspected of being members of a new subspecies. Our results allowed to elucidate a lytic dichotomy based on the lifestyle of A. salmonicida (mesophilic or psychrophilic) and more generally, on phage types (lysotypes) for the subspecies salmonicida. The genomic analyses of the 12 phages from this study with those available in GenBank led us to propose an A. salmonicida phage pan-virome. Our comparative genomic analyses also suggest that some phage genes were under positive selection and A. salmonicida phage genomes having a discrepancy in GC% compared to the host genome encode tRNA genes to likely overpass the bias in codon usage. Finally, we propose a new classification scheme for A. salmonicida phages.Antony T. VincentValérie E. PaquetAlex BernatchezDenise M. TremblaySylvain MoineauSteve J. CharetteNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Antony T. Vincent Valérie E. Paquet Alex Bernatchez Denise M. Tremblay Sylvain Moineau Steve J. Charette Characterization and diversity of phages infecting Aeromonas salmonicida subsp. salmonicida
description	Abstract Phages infecting Aeromonas salmonicida subsp. salmonicida, the causative agent of the fish disease furunculosis, have been isolated for decades but very few of them have been characterized. Here, the host range of 12 virulent phages, including three isolated in the present study, was evaluated against a panel of 65 A. salmonicida isolates, including representatives of the psychrophilic subspecies salmonicida, smithia, masoucida, and the mesophilic subspecies pectinolytica. This bacterial set also included three isolates from India suspected of being members of a new subspecies. Our results allowed to elucidate a lytic dichotomy based on the lifestyle of A. salmonicida (mesophilic or psychrophilic) and more generally, on phage types (lysotypes) for the subspecies salmonicida. The genomic analyses of the 12 phages from this study with those available in GenBank led us to propose an A. salmonicida phage pan-virome. Our comparative genomic analyses also suggest that some phage genes were under positive selection and A. salmonicida phage genomes having a discrepancy in GC% compared to the host genome encode tRNA genes to likely overpass the bias in codon usage. Finally, we propose a new classification scheme for A. salmonicida phages.
format	article
author	Antony T. Vincent Valérie E. Paquet Alex Bernatchez Denise M. Tremblay Sylvain Moineau Steve J. Charette
author_facet	Antony T. Vincent Valérie E. Paquet Alex Bernatchez Denise M. Tremblay Sylvain Moineau Steve J. Charette
author_sort	Antony T. Vincent
title	Characterization and diversity of phages infecting Aeromonas salmonicida subsp. salmonicida
title_short	Characterization and diversity of phages infecting Aeromonas salmonicida subsp. salmonicida
title_full	Characterization and diversity of phages infecting Aeromonas salmonicida subsp. salmonicida
title_fullStr	Characterization and diversity of phages infecting Aeromonas salmonicida subsp. salmonicida
title_full_unstemmed	Characterization and diversity of phages infecting Aeromonas salmonicida subsp. salmonicida
title_sort	characterization and diversity of phages infecting aeromonas salmonicida subsp. salmonicida
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3f8563be5ebb439da3f70d2eb119da81
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Characterization and diversity of phages infecting Aeromonas salmonicida subsp. salmonicida

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