Detection of micrometastases in lung cancer with magnetic nanoparticles and quantum dots

Yali Wang, Yucheng Zhang*, Zhenwu Du, Mei Wu, Guizhen Zhang* Central Laboratory, China-Japan Union Hospital, Jilin University, Changchun, People&rsquo;s Republic of China *These authors contributed equally to this workAbstract: Detection of micrometastases plays an important role in early-st...

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Autores principales:	Wang Y, Zhang Y, Du Z, Wu M, Zhang G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3f8587dd09394da5867c4ff629ff9c8e
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spelling	oai:doaj.org-article:3f8587dd09394da5867c4ff629ff9c8e2021-12-02T05:09:59ZDetection of micrometastases in lung cancer with magnetic nanoparticles and quantum dots1176-91141178-2013https://doaj.org/article/3f8587dd09394da5867c4ff629ff9c8e2012-05-01T00:00:00Zhttp://www.dovepress.com/detection-of-micrometastases-in-lung-cancer-with-magnetic-nanoparticle-a9834https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yali Wang, Yucheng Zhang, Zhenwu Du, Mei Wu, Guizhen Zhang Central Laboratory, China-Japan Union Hospital, Jilin University, Changchun, People&rsquo;s Republic of China *These authors contributed equally to this workAbstract: Detection of micrometastases plays an important role in early-stage and recurrent cancer diagnosis. In the study, a new method of screening micrometastases of lung cancer in peripheral blood by magnetic nanoparticles (MNPs) and quantum dots (QDs) was developed to achieve early diagnosis and recurrence prevention. MNPs were prepared by combining miniemulsion polymerization and St&ouml;ber coating methods. QDs were prepared by using Cd(Ac)2 &bull; 2H2O and oxygen-free NaHTe with thioglycolic acid as the stabilizer. The carbodiimide-mediated condensation method was used to couple pan-cytokeratin (pan-ck) antibody (Ab) to the surface of the MNPs, and Lunx and SP-A Abs to the surface of the QDs. After four kinds of epithelial tumor cells were enriched by MNPs coupled with pan-ck Ab (MNP-pan-ck), lung cancer cells A549 and SPC-A-1 were successfully identified by QDs with double-labeled Abs. Finally, 32 patients with non-small cell lung cancer (NSCLC) were collected, out of 26 cases with the enriched circulating tumor cells (CTCs), 21 cases were successfully identified by QDs. Therefore, a new method was established in which MNP-pan-ck collected CTCs and QDs with double-labeled Abs could be used simultaneously to identify CTCs from NSCLC patients.Keywords: micrometastases, lung cancer, magnetic nanoparticles, quantum dots, Lunx, SP-AWang YZhang YDu ZWu MZhang GDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2315-2324 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wang Y Zhang Y Du Z Wu M Zhang G Detection of micrometastases in lung cancer with magnetic nanoparticles and quantum dots
description	Yali Wang, Yucheng Zhang, Zhenwu Du, Mei Wu, Guizhen Zhang Central Laboratory, China-Japan Union Hospital, Jilin University, Changchun, People&rsquo;s Republic of China *These authors contributed equally to this workAbstract: Detection of micrometastases plays an important role in early-stage and recurrent cancer diagnosis. In the study, a new method of screening micrometastases of lung cancer in peripheral blood by magnetic nanoparticles (MNPs) and quantum dots (QDs) was developed to achieve early diagnosis and recurrence prevention. MNPs were prepared by combining miniemulsion polymerization and St&ouml;ber coating methods. QDs were prepared by using Cd(Ac)2 &bull; 2H2O and oxygen-free NaHTe with thioglycolic acid as the stabilizer. The carbodiimide-mediated condensation method was used to couple pan-cytokeratin (pan-ck) antibody (Ab) to the surface of the MNPs, and Lunx and SP-A Abs to the surface of the QDs. After four kinds of epithelial tumor cells were enriched by MNPs coupled with pan-ck Ab (MNP-pan-ck), lung cancer cells A549 and SPC-A-1 were successfully identified by QDs with double-labeled Abs. Finally, 32 patients with non-small cell lung cancer (NSCLC) were collected, out of 26 cases with the enriched circulating tumor cells (CTCs), 21 cases were successfully identified by QDs. Therefore, a new method was established in which MNP-pan-ck collected CTCs and QDs with double-labeled Abs could be used simultaneously to identify CTCs from NSCLC patients.Keywords: micrometastases, lung cancer, magnetic nanoparticles, quantum dots, Lunx, SP-A
format	article
author	Wang Y Zhang Y Du Z Wu M Zhang G
author_facet	Wang Y Zhang Y Du Z Wu M Zhang G
author_sort	Wang Y
title	Detection of micrometastases in lung cancer with magnetic nanoparticles and quantum dots
title_short	Detection of micrometastases in lung cancer with magnetic nanoparticles and quantum dots
title_full	Detection of micrometastases in lung cancer with magnetic nanoparticles and quantum dots
title_fullStr	Detection of micrometastases in lung cancer with magnetic nanoparticles and quantum dots
title_full_unstemmed	Detection of micrometastases in lung cancer with magnetic nanoparticles and quantum dots
title_sort	detection of micrometastases in lung cancer with magnetic nanoparticles and quantum dots
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/3f8587dd09394da5867c4ff629ff9c8e
work_keys_str_mv	AT wangy detectionofmicrometastasesinlungcancerwithmagneticnanoparticlesandquantumdots AT zhangy detectionofmicrometastasesinlungcancerwithmagneticnanoparticlesandquantumdots AT duz detectionofmicrometastasesinlungcancerwithmagneticnanoparticlesandquantumdots AT wum detectionofmicrometastasesinlungcancerwithmagneticnanoparticlesandquantumdots AT zhangg detectionofmicrometastasesinlungcancerwithmagneticnanoparticlesandquantumdots
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Detection of micrometastases in lung cancer with magnetic nanoparticles and quantum dots

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