Rapid phenotypic individualization of bacterial sister cells

Abstract A growing bacterium typically divides into two genetically identical and morphologically similar sister cells and eventually gives rise to a clonal population. Nevertheless, significant phenotypic differentiation among isogenic cells frequently occurs, with the resulting heterogeneity in ce...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sander K. Govers, Antoine Adam, Hendrik Blockeel, Abram Aertsen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f8fec3b069d40589e5be04908a29a40
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3f8fec3b069d40589e5be04908a29a40
record_format	dspace
spelling	oai:doaj.org-article:3f8fec3b069d40589e5be04908a29a402021-12-02T12:32:08ZRapid phenotypic individualization of bacterial sister cells10.1038/s41598-017-08660-02045-2322https://doaj.org/article/3f8fec3b069d40589e5be04908a29a402017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08660-0https://doaj.org/toc/2045-2322Abstract A growing bacterium typically divides into two genetically identical and morphologically similar sister cells and eventually gives rise to a clonal population. Nevertheless, significant phenotypic differentiation among isogenic cells frequently occurs, with the resulting heterogeneity in cellular behavior often ensuring population level growth and survival in complex and unpredictable environments. Although several mechanisms underlying the generation of phenotypic heterogeneity have been elucidated, the speed with which identical sister cells tend to phenotypically diverge from each other has so far remained unaddressed. Using Escherichia coli as a model organism, we therefore examined the timing and dynamics of phenotypic individualization among sister cells by scrutinizing and modeling microscopically tracked clonally growing populations before and after a semi-lethal heat challenge. This analysis revealed that both survival probability and post-stress physiology of sister cells shift from highly similar to uncorrelated within the first decile of their cell cycles. This nearly-immediate post-fission randomization of sister cell fates highlights the potential of stochastic fluctuations during clonal growth to rapidly generate phenotypically independent individuals.Sander K. GoversAntoine AdamHendrik BlockeelAbram AertsenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sander K. Govers Antoine Adam Hendrik Blockeel Abram Aertsen Rapid phenotypic individualization of bacterial sister cells
description	Abstract A growing bacterium typically divides into two genetically identical and morphologically similar sister cells and eventually gives rise to a clonal population. Nevertheless, significant phenotypic differentiation among isogenic cells frequently occurs, with the resulting heterogeneity in cellular behavior often ensuring population level growth and survival in complex and unpredictable environments. Although several mechanisms underlying the generation of phenotypic heterogeneity have been elucidated, the speed with which identical sister cells tend to phenotypically diverge from each other has so far remained unaddressed. Using Escherichia coli as a model organism, we therefore examined the timing and dynamics of phenotypic individualization among sister cells by scrutinizing and modeling microscopically tracked clonally growing populations before and after a semi-lethal heat challenge. This analysis revealed that both survival probability and post-stress physiology of sister cells shift from highly similar to uncorrelated within the first decile of their cell cycles. This nearly-immediate post-fission randomization of sister cell fates highlights the potential of stochastic fluctuations during clonal growth to rapidly generate phenotypically independent individuals.
format	article
author	Sander K. Govers Antoine Adam Hendrik Blockeel Abram Aertsen
author_facet	Sander K. Govers Antoine Adam Hendrik Blockeel Abram Aertsen
author_sort	Sander K. Govers
title	Rapid phenotypic individualization of bacterial sister cells
title_short	Rapid phenotypic individualization of bacterial sister cells
title_full	Rapid phenotypic individualization of bacterial sister cells
title_fullStr	Rapid phenotypic individualization of bacterial sister cells
title_full_unstemmed	Rapid phenotypic individualization of bacterial sister cells
title_sort	rapid phenotypic individualization of bacterial sister cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3f8fec3b069d40589e5be04908a29a40
work_keys_str_mv	AT sanderkgovers rapidphenotypicindividualizationofbacterialsistercells AT antoineadam rapidphenotypicindividualizationofbacterialsistercells AT hendrikblockeel rapidphenotypicindividualizationofbacterialsistercells AT abramaertsen rapidphenotypicindividualizationofbacterialsistercells
_version_	1718394150004457472

Rapid phenotypic individualization of bacterial sister cells

Ejemplares similares