Telomerase-positive circulating tumor cells are associated with poor prognosis via a neutrophil-mediated inflammatory immune environment in glioma

Abstract Background Gliomas are the most common aggressive cancer in the central nervous system. Considering the difficulty in monitoring glioma response and progression, an approach is needed to evaluate the progression or survival of patients with glioma. We propose an application to facilitate cl...

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Autores principales:	Wen Zhang, Tiancheng Qin, Zhenrong Yang, Liyuan Yin, Changyun Zhao, Lin Feng, Song Lin, Binlei Liu, Shujun Cheng, Kaitai Zhang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Circulating tumor cells Telomerase Prognosis Glioma Neutrophils Immune macroenvironment Medicine R
Acceso en línea:	https://doaj.org/article/3f9330ab146a4664a2e90a634a1500f3
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spelling	oai:doaj.org-article:3f9330ab146a4664a2e90a634a1500f32021-11-14T12:17:04ZTelomerase-positive circulating tumor cells are associated with poor prognosis via a neutrophil-mediated inflammatory immune environment in glioma10.1186/s12916-021-02138-71741-7015https://doaj.org/article/3f9330ab146a4664a2e90a634a1500f32021-11-01T00:00:00Zhttps://doi.org/10.1186/s12916-021-02138-7https://doaj.org/toc/1741-7015Abstract Background Gliomas are the most common aggressive cancer in the central nervous system. Considering the difficulty in monitoring glioma response and progression, an approach is needed to evaluate the progression or survival of patients with glioma. We propose an application to facilitate clinical detection and treatment monitoring in glioma patients by using telomerase-positive circulating tumor cells (CTCs) and to further evaluate the relationship between the immune microenvironment and CTCs in glioma patients. Methods From October 2014 to June 2017, 106 patients newly diagnosed with glioma were enrolled. We used the telomerase reverse transcriptase CTC detection method to detect and analyze the CTC statuses of glioma patients before and after surgery. FlowSight and FISH confirmed the CTCs detected by the telomerase-based method. To verify the correlation between CTCs and the immune response, peripheral white blood cell RNA sequencing was performed. Results CTCs were common in the peripheral blood of glioma patients and were not correlated with the pathological classification or grade of patients. The results showed that the presence of postoperative CTCs but not preoperative CTCs in glioma patients was a poor prognostic factor. The level of postoperative CTCs, which predicts a poor prognosis after surgery, may be associated with neutrophils. RNA sequencing suggested that postoperative CTCs were positively correlated with innate immune responses, especially the activation of neutrophils and the generation of neutrophil extracellular traps, but negatively correlated with the cytotoxic response. Conclusions Our results showed that telomerase-positive CTCs can predict a poor prognosis of patients with glioma. Our results also showed a correlation between CTCs and the immune macroenvironment, which provides a new perspective for the treatment of glioma.Wen ZhangTiancheng QinZhenrong YangLiyuan YinChangyun ZhaoLin FengSong LinBinlei LiuShujun ChengKaitai ZhangBMCarticleCirculating tumor cellsTelomerasePrognosisGliomaNeutrophilsImmune macroenvironmentMedicineRENBMC Medicine, Vol 19, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Circulating tumor cells Telomerase Prognosis Glioma Neutrophils Immune macroenvironment Medicine R
spellingShingle	Circulating tumor cells Telomerase Prognosis Glioma Neutrophils Immune macroenvironment Medicine R Wen Zhang Tiancheng Qin Zhenrong Yang Liyuan Yin Changyun Zhao Lin Feng Song Lin Binlei Liu Shujun Cheng Kaitai Zhang Telomerase-positive circulating tumor cells are associated with poor prognosis via a neutrophil-mediated inflammatory immune environment in glioma
description	Abstract Background Gliomas are the most common aggressive cancer in the central nervous system. Considering the difficulty in monitoring glioma response and progression, an approach is needed to evaluate the progression or survival of patients with glioma. We propose an application to facilitate clinical detection and treatment monitoring in glioma patients by using telomerase-positive circulating tumor cells (CTCs) and to further evaluate the relationship between the immune microenvironment and CTCs in glioma patients. Methods From October 2014 to June 2017, 106 patients newly diagnosed with glioma were enrolled. We used the telomerase reverse transcriptase CTC detection method to detect and analyze the CTC statuses of glioma patients before and after surgery. FlowSight and FISH confirmed the CTCs detected by the telomerase-based method. To verify the correlation between CTCs and the immune response, peripheral white blood cell RNA sequencing was performed. Results CTCs were common in the peripheral blood of glioma patients and were not correlated with the pathological classification or grade of patients. The results showed that the presence of postoperative CTCs but not preoperative CTCs in glioma patients was a poor prognostic factor. The level of postoperative CTCs, which predicts a poor prognosis after surgery, may be associated with neutrophils. RNA sequencing suggested that postoperative CTCs were positively correlated with innate immune responses, especially the activation of neutrophils and the generation of neutrophil extracellular traps, but negatively correlated with the cytotoxic response. Conclusions Our results showed that telomerase-positive CTCs can predict a poor prognosis of patients with glioma. Our results also showed a correlation between CTCs and the immune macroenvironment, which provides a new perspective for the treatment of glioma.
format	article
author	Wen Zhang Tiancheng Qin Zhenrong Yang Liyuan Yin Changyun Zhao Lin Feng Song Lin Binlei Liu Shujun Cheng Kaitai Zhang
author_facet	Wen Zhang Tiancheng Qin Zhenrong Yang Liyuan Yin Changyun Zhao Lin Feng Song Lin Binlei Liu Shujun Cheng Kaitai Zhang
author_sort	Wen Zhang
title	Telomerase-positive circulating tumor cells are associated with poor prognosis via a neutrophil-mediated inflammatory immune environment in glioma
title_short	Telomerase-positive circulating tumor cells are associated with poor prognosis via a neutrophil-mediated inflammatory immune environment in glioma
title_full	Telomerase-positive circulating tumor cells are associated with poor prognosis via a neutrophil-mediated inflammatory immune environment in glioma
title_fullStr	Telomerase-positive circulating tumor cells are associated with poor prognosis via a neutrophil-mediated inflammatory immune environment in glioma
title_full_unstemmed	Telomerase-positive circulating tumor cells are associated with poor prognosis via a neutrophil-mediated inflammatory immune environment in glioma
title_sort	telomerase-positive circulating tumor cells are associated with poor prognosis via a neutrophil-mediated inflammatory immune environment in glioma
publisher	BMC
publishDate	2021
url	https://doaj.org/article/3f9330ab146a4664a2e90a634a1500f3
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Telomerase-positive circulating tumor cells are associated with poor prognosis via a neutrophil-mediated inflammatory immune environment in glioma

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