Potential role for the Metnase transposase fusion gene in colon cancer through the regulation of key genes.

The Metnase fusion gene consists of a SET histone methyltransferase domain and a transposase domain from Mariner transposase. This transposable element is involved in chromosome decatenation, enhances DNA repair, promotes foreign DNA integration, and assists topoisomerase II function. This study inv...

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Autores principales:	Panagiotis Apostolou, Maria Toloudi, Eleni Kourtidou, Georgia Mimikakou, Ioanna Vlachou, Marina Chatziioannou, Vasiliki Kipourou, Ioannis Papasotiriou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f97b9917c734fb9b28f3af0f987cf1f
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spelling	oai:doaj.org-article:3f97b9917c734fb9b28f3af0f987cf1f2021-11-25T05:56:32ZPotential role for the Metnase transposase fusion gene in colon cancer through the regulation of key genes.1932-620310.1371/journal.pone.0109741https://doaj.org/article/3f97b9917c734fb9b28f3af0f987cf1f2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109741https://doaj.org/toc/1932-6203The Metnase fusion gene consists of a SET histone methyltransferase domain and a transposase domain from Mariner transposase. This transposable element is involved in chromosome decatenation, enhances DNA repair, promotes foreign DNA integration, and assists topoisomerase II function. This study investigates the role of Metnase in colon cancer homeostasis and maintenance of the stemness phenotype in colon cancer stem cells (CSCs). Silencing of Metnase was performed in human cancer cell lines before and after treatment with cisplatin, and in colon CSCs. Subsequent changes in the expression of genes involved in repair mechanisms, DNA synthesis, topoisomerase II function, and metastasis as well stemness transcription factors were studied with RT-qPCR experiments. Cellular viability and apoptosis were evaluated by flow cytometry. The results suggest that Metnase influences the expression of many genes involved in the above processes. Furthermore, Metnase levels appear to impact upon expression of NANOG, OCT3/4, and SOX2. Suppression of Metnase also led to an increase in apoptosis. Therefore, Metnase may possess an important role in DNA repair, topoisomerase II function, and the maintenance of stemness during colon cancer development.Panagiotis ApostolouMaria ToloudiEleni KourtidouGeorgia MimikakouIoanna VlachouMarina ChatziioannouVasiliki KipourouIoannis PapasotiriouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109741 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Panagiotis Apostolou Maria Toloudi Eleni Kourtidou Georgia Mimikakou Ioanna Vlachou Marina Chatziioannou Vasiliki Kipourou Ioannis Papasotiriou Potential role for the Metnase transposase fusion gene in colon cancer through the regulation of key genes.
description	The Metnase fusion gene consists of a SET histone methyltransferase domain and a transposase domain from Mariner transposase. This transposable element is involved in chromosome decatenation, enhances DNA repair, promotes foreign DNA integration, and assists topoisomerase II function. This study investigates the role of Metnase in colon cancer homeostasis and maintenance of the stemness phenotype in colon cancer stem cells (CSCs). Silencing of Metnase was performed in human cancer cell lines before and after treatment with cisplatin, and in colon CSCs. Subsequent changes in the expression of genes involved in repair mechanisms, DNA synthesis, topoisomerase II function, and metastasis as well stemness transcription factors were studied with RT-qPCR experiments. Cellular viability and apoptosis were evaluated by flow cytometry. The results suggest that Metnase influences the expression of many genes involved in the above processes. Furthermore, Metnase levels appear to impact upon expression of NANOG, OCT3/4, and SOX2. Suppression of Metnase also led to an increase in apoptosis. Therefore, Metnase may possess an important role in DNA repair, topoisomerase II function, and the maintenance of stemness during colon cancer development.
format	article
author	Panagiotis Apostolou Maria Toloudi Eleni Kourtidou Georgia Mimikakou Ioanna Vlachou Marina Chatziioannou Vasiliki Kipourou Ioannis Papasotiriou
author_facet	Panagiotis Apostolou Maria Toloudi Eleni Kourtidou Georgia Mimikakou Ioanna Vlachou Marina Chatziioannou Vasiliki Kipourou Ioannis Papasotiriou
author_sort	Panagiotis Apostolou
title	Potential role for the Metnase transposase fusion gene in colon cancer through the regulation of key genes.
title_short	Potential role for the Metnase transposase fusion gene in colon cancer through the regulation of key genes.
title_full	Potential role for the Metnase transposase fusion gene in colon cancer through the regulation of key genes.
title_fullStr	Potential role for the Metnase transposase fusion gene in colon cancer through the regulation of key genes.
title_full_unstemmed	Potential role for the Metnase transposase fusion gene in colon cancer through the regulation of key genes.
title_sort	potential role for the metnase transposase fusion gene in colon cancer through the regulation of key genes.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/3f97b9917c734fb9b28f3af0f987cf1f
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Potential role for the Metnase transposase fusion gene in colon cancer through the regulation of key genes.

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