Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease

Abstract The precise role of periostin, an extra-cellular matrix protein, in inflammatory bowel disease (IBD) is unclear. Here, we investigated periostin in paediatric IBD including its relationship with disease activity, clinical outcomes, genomic variation and expression in the colonic tissue. Pla...

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Autores principales: Tracy Coelho, Eva Sonnenberg-Riethmacher, Yifang Gao, Enrico Mossotto, Alisher Khojanazarov, Annie Griffin, Saida Mukanova, Aiymkul Ashimkhanova, Rachel Haggarty, Anton Borissenko, James J. Ashton, Imogen S. Stafford, Akshay Batra, Nadeem A. Afzal, Michael P. Stanton, Bhumita Vadgama, Kapura Adrisova, Robert M. Beattie, Anthony P. Williams, Sarah Ennis, Dieter Riethmacher
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Publicado: Nature Portfolio 2021
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spelling oai:doaj.org-article:3f97eee59cb1479faae9c5a30eaf5c4d2021-12-02T11:39:48ZExpression profile of the matricellular protein periostin in paediatric inflammatory bowel disease10.1038/s41598-021-85096-72045-2322https://doaj.org/article/3f97eee59cb1479faae9c5a30eaf5c4d2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85096-7https://doaj.org/toc/2045-2322Abstract The precise role of periostin, an extra-cellular matrix protein, in inflammatory bowel disease (IBD) is unclear. Here, we investigated periostin in paediatric IBD including its relationship with disease activity, clinical outcomes, genomic variation and expression in the colonic tissue. Plasma periostin was analysed using ELISA in 144 paediatric patients and 38 controls. Plasma levels were assessed against validated disease activity indices in IBD and clinical outcomes. An immuno-fluorescence for periostin and detailed isoform-expression analysis in the colonic tissue was performed in 23 individuals. We integrated a whole-gene based burden metric ‘GenePy’ to assess the impact of variation in POSTN and 23 other genes functionally connected to periostin. We found that plasma periostin levels were significantly increased during remission compared to active Crohn’s disease. The immuno-fluorescence analysis demonstrated enhanced peri-cryptal ring patterns in patients compared to controls, present throughout inflamed, as well as macroscopically non-inflamed colonic tissue. Interestingly, the pattern of isoforms remained unchanged during bowel inflammation compared to healthy controls. In addition to its role during the inflammatory processes in IBD, periostin may have an additional prominent role in mucosal repair. Additional studies will be necessary to understand its role in the pathogenesis, repair and fibrosis in IBD.Tracy CoelhoEva Sonnenberg-RiethmacherYifang GaoEnrico MossottoAlisher KhojanazarovAnnie GriffinSaida MukanovaAiymkul AshimkhanovaRachel HaggartyAnton BorissenkoJames J. AshtonImogen S. StaffordAkshay BatraNadeem A. AfzalMichael P. StantonBhumita VadgamaKapura AdrisovaRobert M. BeattieAnthony P. WilliamsSarah EnnisDieter RiethmacherNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Tracy Coelho
Eva Sonnenberg-Riethmacher
Yifang Gao
Enrico Mossotto
Alisher Khojanazarov
Annie Griffin
Saida Mukanova
Aiymkul Ashimkhanova
Rachel Haggarty
Anton Borissenko
James J. Ashton
Imogen S. Stafford
Akshay Batra
Nadeem A. Afzal
Michael P. Stanton
Bhumita Vadgama
Kapura Adrisova
Robert M. Beattie
Anthony P. Williams
Sarah Ennis
Dieter Riethmacher
Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
description Abstract The precise role of periostin, an extra-cellular matrix protein, in inflammatory bowel disease (IBD) is unclear. Here, we investigated periostin in paediatric IBD including its relationship with disease activity, clinical outcomes, genomic variation and expression in the colonic tissue. Plasma periostin was analysed using ELISA in 144 paediatric patients and 38 controls. Plasma levels were assessed against validated disease activity indices in IBD and clinical outcomes. An immuno-fluorescence for periostin and detailed isoform-expression analysis in the colonic tissue was performed in 23 individuals. We integrated a whole-gene based burden metric ‘GenePy’ to assess the impact of variation in POSTN and 23 other genes functionally connected to periostin. We found that plasma periostin levels were significantly increased during remission compared to active Crohn’s disease. The immuno-fluorescence analysis demonstrated enhanced peri-cryptal ring patterns in patients compared to controls, present throughout inflamed, as well as macroscopically non-inflamed colonic tissue. Interestingly, the pattern of isoforms remained unchanged during bowel inflammation compared to healthy controls. In addition to its role during the inflammatory processes in IBD, periostin may have an additional prominent role in mucosal repair. Additional studies will be necessary to understand its role in the pathogenesis, repair and fibrosis in IBD.
format article
author Tracy Coelho
Eva Sonnenberg-Riethmacher
Yifang Gao
Enrico Mossotto
Alisher Khojanazarov
Annie Griffin
Saida Mukanova
Aiymkul Ashimkhanova
Rachel Haggarty
Anton Borissenko
James J. Ashton
Imogen S. Stafford
Akshay Batra
Nadeem A. Afzal
Michael P. Stanton
Bhumita Vadgama
Kapura Adrisova
Robert M. Beattie
Anthony P. Williams
Sarah Ennis
Dieter Riethmacher
author_facet Tracy Coelho
Eva Sonnenberg-Riethmacher
Yifang Gao
Enrico Mossotto
Alisher Khojanazarov
Annie Griffin
Saida Mukanova
Aiymkul Ashimkhanova
Rachel Haggarty
Anton Borissenko
James J. Ashton
Imogen S. Stafford
Akshay Batra
Nadeem A. Afzal
Michael P. Stanton
Bhumita Vadgama
Kapura Adrisova
Robert M. Beattie
Anthony P. Williams
Sarah Ennis
Dieter Riethmacher
author_sort Tracy Coelho
title Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
title_short Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
title_full Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
title_fullStr Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
title_full_unstemmed Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
title_sort expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/3f97eee59cb1479faae9c5a30eaf5c4d
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