Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease

Abstract The precise role of periostin, an extra-cellular matrix protein, in inflammatory bowel disease (IBD) is unclear. Here, we investigated periostin in paediatric IBD including its relationship with disease activity, clinical outcomes, genomic variation and expression in the colonic tissue. Pla...

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Autores principales:	Tracy Coelho, Eva Sonnenberg-Riethmacher, Yifang Gao, Enrico Mossotto, Alisher Khojanazarov, Annie Griffin, Saida Mukanova, Aiymkul Ashimkhanova, Rachel Haggarty, Anton Borissenko, James J. Ashton, Imogen S. Stafford, Akshay Batra, Nadeem A. Afzal, Michael P. Stanton, Bhumita Vadgama, Kapura Adrisova, Robert M. Beattie, Anthony P. Williams, Sarah Ennis, Dieter Riethmacher
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3f97eee59cb1479faae9c5a30eaf5c4d
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spelling	oai:doaj.org-article:3f97eee59cb1479faae9c5a30eaf5c4d2021-12-02T11:39:48ZExpression profile of the matricellular protein periostin in paediatric inflammatory bowel disease10.1038/s41598-021-85096-72045-2322https://doaj.org/article/3f97eee59cb1479faae9c5a30eaf5c4d2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85096-7https://doaj.org/toc/2045-2322Abstract The precise role of periostin, an extra-cellular matrix protein, in inflammatory bowel disease (IBD) is unclear. Here, we investigated periostin in paediatric IBD including its relationship with disease activity, clinical outcomes, genomic variation and expression in the colonic tissue. Plasma periostin was analysed using ELISA in 144 paediatric patients and 38 controls. Plasma levels were assessed against validated disease activity indices in IBD and clinical outcomes. An immuno-fluorescence for periostin and detailed isoform-expression analysis in the colonic tissue was performed in 23 individuals. We integrated a whole-gene based burden metric ‘GenePy’ to assess the impact of variation in POSTN and 23 other genes functionally connected to periostin. We found that plasma periostin levels were significantly increased during remission compared to active Crohn’s disease. The immuno-fluorescence analysis demonstrated enhanced peri-cryptal ring patterns in patients compared to controls, present throughout inflamed, as well as macroscopically non-inflamed colonic tissue. Interestingly, the pattern of isoforms remained unchanged during bowel inflammation compared to healthy controls. In addition to its role during the inflammatory processes in IBD, periostin may have an additional prominent role in mucosal repair. Additional studies will be necessary to understand its role in the pathogenesis, repair and fibrosis in IBD.Tracy CoelhoEva Sonnenberg-RiethmacherYifang GaoEnrico MossottoAlisher KhojanazarovAnnie GriffinSaida MukanovaAiymkul AshimkhanovaRachel HaggartyAnton BorissenkoJames J. AshtonImogen S. StaffordAkshay BatraNadeem A. AfzalMichael P. StantonBhumita VadgamaKapura AdrisovaRobert M. BeattieAnthony P. WilliamsSarah EnnisDieter RiethmacherNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tracy Coelho Eva Sonnenberg-Riethmacher Yifang Gao Enrico Mossotto Alisher Khojanazarov Annie Griffin Saida Mukanova Aiymkul Ashimkhanova Rachel Haggarty Anton Borissenko James J. Ashton Imogen S. Stafford Akshay Batra Nadeem A. Afzal Michael P. Stanton Bhumita Vadgama Kapura Adrisova Robert M. Beattie Anthony P. Williams Sarah Ennis Dieter Riethmacher Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
description	Abstract The precise role of periostin, an extra-cellular matrix protein, in inflammatory bowel disease (IBD) is unclear. Here, we investigated periostin in paediatric IBD including its relationship with disease activity, clinical outcomes, genomic variation and expression in the colonic tissue. Plasma periostin was analysed using ELISA in 144 paediatric patients and 38 controls. Plasma levels were assessed against validated disease activity indices in IBD and clinical outcomes. An immuno-fluorescence for periostin and detailed isoform-expression analysis in the colonic tissue was performed in 23 individuals. We integrated a whole-gene based burden metric ‘GenePy’ to assess the impact of variation in POSTN and 23 other genes functionally connected to periostin. We found that plasma periostin levels were significantly increased during remission compared to active Crohn’s disease. The immuno-fluorescence analysis demonstrated enhanced peri-cryptal ring patterns in patients compared to controls, present throughout inflamed, as well as macroscopically non-inflamed colonic tissue. Interestingly, the pattern of isoforms remained unchanged during bowel inflammation compared to healthy controls. In addition to its role during the inflammatory processes in IBD, periostin may have an additional prominent role in mucosal repair. Additional studies will be necessary to understand its role in the pathogenesis, repair and fibrosis in IBD.
format	article
author	Tracy Coelho Eva Sonnenberg-Riethmacher Yifang Gao Enrico Mossotto Alisher Khojanazarov Annie Griffin Saida Mukanova Aiymkul Ashimkhanova Rachel Haggarty Anton Borissenko James J. Ashton Imogen S. Stafford Akshay Batra Nadeem A. Afzal Michael P. Stanton Bhumita Vadgama Kapura Adrisova Robert M. Beattie Anthony P. Williams Sarah Ennis Dieter Riethmacher
author_facet	Tracy Coelho Eva Sonnenberg-Riethmacher Yifang Gao Enrico Mossotto Alisher Khojanazarov Annie Griffin Saida Mukanova Aiymkul Ashimkhanova Rachel Haggarty Anton Borissenko James J. Ashton Imogen S. Stafford Akshay Batra Nadeem A. Afzal Michael P. Stanton Bhumita Vadgama Kapura Adrisova Robert M. Beattie Anthony P. Williams Sarah Ennis Dieter Riethmacher
author_sort	Tracy Coelho
title	Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
title_short	Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
title_full	Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
title_fullStr	Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
title_full_unstemmed	Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
title_sort	expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3f97eee59cb1479faae9c5a30eaf5c4d
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Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease

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