Monitoring and molecular characterization of bacterial species in heavy metals contaminated roadside soil of selected region along NH 8A, Gujarat

Heavy metal contamination is a universal concern due to health risks associated with metal pollution. Soil contamination by heavy metals is known to affect microbial activities at elevated concentrations adversely. However, indigenous soil bacterial populations' response to added heavy metal an...

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Autores principales:	Snigdha Singh, R.Y. Hiranmai
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Microbial diversity Heavy metal pollution 16S-rRNA sequencing Phylogenetic tree Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/3f9cc17c81ce487786004ba8b6199af5
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spelling	oai:doaj.org-article:3f9cc17c81ce487786004ba8b6199af52021-12-02T05:02:26ZMonitoring and molecular characterization of bacterial species in heavy metals contaminated roadside soil of selected region along NH 8A, Gujarat2405-844010.1016/j.heliyon.2021.e08284https://doaj.org/article/3f9cc17c81ce487786004ba8b6199af52021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405844021023872https://doaj.org/toc/2405-8440Heavy metal contamination is a universal concern due to health risks associated with metal pollution. Soil contamination by heavy metals is known to affect microbial activities at elevated concentrations adversely. However, indigenous soil bacterial populations' response to added heavy metal and metal combinations is poorly understood. Microbes prevailing in the soil are the driving factors. Their properties are recognized as sensitive indicators of soil quality and health. Moreover, these microscopic organisms are accountable for the fertility and aeration of the soil that forms fundamental aspects of soil function. The current study was performed to explore the diversity of bacterial species in heavy metal polluted roadside soil. The roadside soil samples were collected from diverse sites and processed for physicochemical properties, microbial characterization, and heavy metals distribution in the selected locations. Serial dilution and spread plate techniques were used for the isolation of bacterial species. The 16S-rRNA gene sequencing identified bacterial species in roadside soil as Bacillus drentensis (MK217088), Bacillus safensis (MK774729), Bacillus haynesii (MK192808), Bacillus subtilis (MK217089), and Bacillus cereus (MK801278). In addition, the 16S rRNA sequences of isolated bacterial strains were aligned to generate a phylogenetic tree. Thus, the current research study provides a platform for efficiently investigating roadside soils by microbial profiling that may discover novel microbes of scientific significance and improved potential.Snigdha SinghR.Y. HiranmaiElsevierarticleMicrobial diversityHeavy metal pollution16S-rRNA sequencingPhylogenetic treeScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 11, Pp e08284- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial diversity Heavy metal pollution 16S-rRNA sequencing Phylogenetic tree Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	Microbial diversity Heavy metal pollution 16S-rRNA sequencing Phylogenetic tree Science (General) Q1-390 Social sciences (General) H1-99 Snigdha Singh R.Y. Hiranmai Monitoring and molecular characterization of bacterial species in heavy metals contaminated roadside soil of selected region along NH 8A, Gujarat
description	Heavy metal contamination is a universal concern due to health risks associated with metal pollution. Soil contamination by heavy metals is known to affect microbial activities at elevated concentrations adversely. However, indigenous soil bacterial populations' response to added heavy metal and metal combinations is poorly understood. Microbes prevailing in the soil are the driving factors. Their properties are recognized as sensitive indicators of soil quality and health. Moreover, these microscopic organisms are accountable for the fertility and aeration of the soil that forms fundamental aspects of soil function. The current study was performed to explore the diversity of bacterial species in heavy metal polluted roadside soil. The roadside soil samples were collected from diverse sites and processed for physicochemical properties, microbial characterization, and heavy metals distribution in the selected locations. Serial dilution and spread plate techniques were used for the isolation of bacterial species. The 16S-rRNA gene sequencing identified bacterial species in roadside soil as Bacillus drentensis (MK217088), Bacillus safensis (MK774729), Bacillus haynesii (MK192808), Bacillus subtilis (MK217089), and Bacillus cereus (MK801278). In addition, the 16S rRNA sequences of isolated bacterial strains were aligned to generate a phylogenetic tree. Thus, the current research study provides a platform for efficiently investigating roadside soils by microbial profiling that may discover novel microbes of scientific significance and improved potential.
format	article
author	Snigdha Singh R.Y. Hiranmai
author_facet	Snigdha Singh R.Y. Hiranmai
author_sort	Snigdha Singh
title	Monitoring and molecular characterization of bacterial species in heavy metals contaminated roadside soil of selected region along NH 8A, Gujarat
title_short	Monitoring and molecular characterization of bacterial species in heavy metals contaminated roadside soil of selected region along NH 8A, Gujarat
title_full	Monitoring and molecular characterization of bacterial species in heavy metals contaminated roadside soil of selected region along NH 8A, Gujarat
title_fullStr	Monitoring and molecular characterization of bacterial species in heavy metals contaminated roadside soil of selected region along NH 8A, Gujarat
title_full_unstemmed	Monitoring and molecular characterization of bacterial species in heavy metals contaminated roadside soil of selected region along NH 8A, Gujarat
title_sort	monitoring and molecular characterization of bacterial species in heavy metals contaminated roadside soil of selected region along nh 8a, gujarat
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/3f9cc17c81ce487786004ba8b6199af5
work_keys_str_mv	AT snigdhasingh monitoringandmolecularcharacterizationofbacterialspeciesinheavymetalscontaminatedroadsidesoilofselectedregionalongnh8agujarat AT ryhiranmai monitoringandmolecularcharacterizationofbacterialspeciesinheavymetalscontaminatedroadsidesoilofselectedregionalongnh8agujarat
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Monitoring and molecular characterization of bacterial species in heavy metals contaminated roadside soil of selected region along NH 8A, Gujarat

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