MPT64 antigen detection test improves diagnosis of pediatric extrapulmonary tuberculosis in Mbeya, Tanzania

Abstract Pediatric extrapulmonary tuberculosis (EPTB) is a diagnostic challenge. A new immunochemistry based MPT64 antigen detection test has shown improved sensitivity compared to current laboratory tests. The aim of this study was to implement and validate the test performance in a resource limite...

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Autores principales: Erlend Grønningen, Marywinnie Nanyaro, Lisbet Sviland, Esther Ngadaya, William Muller, Lisete Torres, Sayoki Mfinanga, Tehmina Mustafa
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Publicado: Nature Portfolio 2021
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spelling oai:doaj.org-article:3fa65131173241629370dd43c7efb9792021-12-02T16:38:49ZMPT64 antigen detection test improves diagnosis of pediatric extrapulmonary tuberculosis in Mbeya, Tanzania10.1038/s41598-021-97010-22045-2322https://doaj.org/article/3fa65131173241629370dd43c7efb9792021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97010-2https://doaj.org/toc/2045-2322Abstract Pediatric extrapulmonary tuberculosis (EPTB) is a diagnostic challenge. A new immunochemistry based MPT64 antigen detection test has shown improved sensitivity compared to current laboratory tests. The aim of this study was to implement and validate the test performance in a resource limited African setting. Presumptive pediatric (0–18 y) EPTB patients were prospectively enrolled at Mbeya Zonal Referral Hospital, and followed to the end of treatment or until a final diagnosis was reached. Specimens from suspected sites of infection were subject to routine diagnostics, GeneXpert MTB/RIF assay and the MPT64 test. The performance of the tests was assessed using mycobacterial culture as well as a composite reference standard. 30 patients were categorized as TB cases, 31 as non-TB cases and 2 were uncategorized. In the TB group, the three most common infections were adenitis (30%), peritonitis (30%) and meningitis (20%). The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of the MPT64 test was 92%, 88%, 87%, 92% and 90%, respectively. Mortality was equally high among TB/non-TB cases (23% vs 21%), and malnutrition was the main comorbidity among TB cases. The MPT64 test was implementable in the routine diagnostics in a low-resource setting and improved the diagnosis of pediatric EPTB.Erlend GrønningenMarywinnie NanyaroLisbet SvilandEsther NgadayaWilliam MullerLisete TorresSayoki MfinangaTehmina MustafaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Erlend Grønningen
Marywinnie Nanyaro
Lisbet Sviland
Esther Ngadaya
William Muller
Lisete Torres
Sayoki Mfinanga
Tehmina Mustafa
MPT64 antigen detection test improves diagnosis of pediatric extrapulmonary tuberculosis in Mbeya, Tanzania
description Abstract Pediatric extrapulmonary tuberculosis (EPTB) is a diagnostic challenge. A new immunochemistry based MPT64 antigen detection test has shown improved sensitivity compared to current laboratory tests. The aim of this study was to implement and validate the test performance in a resource limited African setting. Presumptive pediatric (0–18 y) EPTB patients were prospectively enrolled at Mbeya Zonal Referral Hospital, and followed to the end of treatment or until a final diagnosis was reached. Specimens from suspected sites of infection were subject to routine diagnostics, GeneXpert MTB/RIF assay and the MPT64 test. The performance of the tests was assessed using mycobacterial culture as well as a composite reference standard. 30 patients were categorized as TB cases, 31 as non-TB cases and 2 were uncategorized. In the TB group, the three most common infections were adenitis (30%), peritonitis (30%) and meningitis (20%). The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of the MPT64 test was 92%, 88%, 87%, 92% and 90%, respectively. Mortality was equally high among TB/non-TB cases (23% vs 21%), and malnutrition was the main comorbidity among TB cases. The MPT64 test was implementable in the routine diagnostics in a low-resource setting and improved the diagnosis of pediatric EPTB.
format article
author Erlend Grønningen
Marywinnie Nanyaro
Lisbet Sviland
Esther Ngadaya
William Muller
Lisete Torres
Sayoki Mfinanga
Tehmina Mustafa
author_facet Erlend Grønningen
Marywinnie Nanyaro
Lisbet Sviland
Esther Ngadaya
William Muller
Lisete Torres
Sayoki Mfinanga
Tehmina Mustafa
author_sort Erlend Grønningen
title MPT64 antigen detection test improves diagnosis of pediatric extrapulmonary tuberculosis in Mbeya, Tanzania
title_short MPT64 antigen detection test improves diagnosis of pediatric extrapulmonary tuberculosis in Mbeya, Tanzania
title_full MPT64 antigen detection test improves diagnosis of pediatric extrapulmonary tuberculosis in Mbeya, Tanzania
title_fullStr MPT64 antigen detection test improves diagnosis of pediatric extrapulmonary tuberculosis in Mbeya, Tanzania
title_full_unstemmed MPT64 antigen detection test improves diagnosis of pediatric extrapulmonary tuberculosis in Mbeya, Tanzania
title_sort mpt64 antigen detection test improves diagnosis of pediatric extrapulmonary tuberculosis in mbeya, tanzania
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/3fa65131173241629370dd43c7efb979
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