Characterization of SARS-CoV-2 Variants N501Y.V1 and N501Y.V2 Spike on Viral Infectivity

SARS-coronavirus 2 (SARS-CoV-2), pathogen of coronavirus disease 2019 (COVID-19), is constantly evolving to adapt to the host and evade antiviral immunity. The newly emerging variants N501Y.V1 (B.1.1.7) and N501Y.V2 (B.1.351), first reported in the United Kingdom and South Africa respectively, raise...

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Autores principales:	Haijun Tang, Long Gao, Zhao Wu, Fang Meng, Xin Zhao, Yun Shao, Xiaohua Shi, Shigang Qiao, Jianzhong An, Xiaohong Du, F. Xiao-Feng Qin
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	SARS-CoV-2 N501Y.V1 N501Y.V2 infectivity thermal stability Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/3fa689bba3a2489e91661625c168c93b
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spelling	oai:doaj.org-article:3fa689bba3a2489e91661625c168c93b2021-12-01T14:29:37ZCharacterization of SARS-CoV-2 Variants N501Y.V1 and N501Y.V2 Spike on Viral Infectivity2235-298810.3389/fcimb.2021.720357https://doaj.org/article/3fa689bba3a2489e91661625c168c93b2021-10-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.720357/fullhttps://doaj.org/toc/2235-2988SARS-coronavirus 2 (SARS-CoV-2), pathogen of coronavirus disease 2019 (COVID-19), is constantly evolving to adapt to the host and evade antiviral immunity. The newly emerging variants N501Y.V1 (B.1.1.7) and N501Y.V2 (B.1.351), first reported in the United Kingdom and South Africa respectively, raised concerns due to the unusually rapid global spread. The mutations in spike (S) protein may contribute to the rapid spread of these variants. Here, with a vesicular stomatitis virus (VSV)-based pseudotype system, we demonstrated that the pseudovirus bearing N501Y.V2 S protein has higher infection efficiency than pseudovirus with wildtype (WT) and D614G S protein. Moreover, pseudovirus with N501Y.V1 or N501Y.V2 S protein has better thermal stability than WT and D614G, suggesting these mutations of variants may increase the stability of SARS-CoV-2 S protein and virion. However, the pseudovirus bearing N501Y.V1 or N501Y.V2 S protein has similar sensitivity to inhibitors of protease and endocytosis with WT and D614G. These findings could be of value in preventing the spread of virus and developing drugs for emerging SARS-CoV-2 variants.Haijun TangHaijun TangLong GaoLong GaoZhao WuZhao WuFang MengFang MengXin ZhaoXin ZhaoYun ShaoYun ShaoXiaohua ShiShigang QiaoJianzhong AnXiaohong DuF. Xiao-Feng QinF. Xiao-Feng QinFrontiers Media S.A.articleSARS-CoV-2N501Y.V1N501Y.V2infectivitythermal stabilityMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 N501Y.V1 N501Y.V2 infectivity thermal stability Microbiology QR1-502
spellingShingle	SARS-CoV-2 N501Y.V1 N501Y.V2 infectivity thermal stability Microbiology QR1-502 Haijun Tang Haijun Tang Long Gao Long Gao Zhao Wu Zhao Wu Fang Meng Fang Meng Xin Zhao Xin Zhao Yun Shao Yun Shao Xiaohua Shi Shigang Qiao Jianzhong An Xiaohong Du F. Xiao-Feng Qin F. Xiao-Feng Qin Characterization of SARS-CoV-2 Variants N501Y.V1 and N501Y.V2 Spike on Viral Infectivity
description	SARS-coronavirus 2 (SARS-CoV-2), pathogen of coronavirus disease 2019 (COVID-19), is constantly evolving to adapt to the host and evade antiviral immunity. The newly emerging variants N501Y.V1 (B.1.1.7) and N501Y.V2 (B.1.351), first reported in the United Kingdom and South Africa respectively, raised concerns due to the unusually rapid global spread. The mutations in spike (S) protein may contribute to the rapid spread of these variants. Here, with a vesicular stomatitis virus (VSV)-based pseudotype system, we demonstrated that the pseudovirus bearing N501Y.V2 S protein has higher infection efficiency than pseudovirus with wildtype (WT) and D614G S protein. Moreover, pseudovirus with N501Y.V1 or N501Y.V2 S protein has better thermal stability than WT and D614G, suggesting these mutations of variants may increase the stability of SARS-CoV-2 S protein and virion. However, the pseudovirus bearing N501Y.V1 or N501Y.V2 S protein has similar sensitivity to inhibitors of protease and endocytosis with WT and D614G. These findings could be of value in preventing the spread of virus and developing drugs for emerging SARS-CoV-2 variants.
format	article
author	Haijun Tang Haijun Tang Long Gao Long Gao Zhao Wu Zhao Wu Fang Meng Fang Meng Xin Zhao Xin Zhao Yun Shao Yun Shao Xiaohua Shi Shigang Qiao Jianzhong An Xiaohong Du F. Xiao-Feng Qin F. Xiao-Feng Qin
author_facet	Haijun Tang Haijun Tang Long Gao Long Gao Zhao Wu Zhao Wu Fang Meng Fang Meng Xin Zhao Xin Zhao Yun Shao Yun Shao Xiaohua Shi Shigang Qiao Jianzhong An Xiaohong Du F. Xiao-Feng Qin F. Xiao-Feng Qin
author_sort	Haijun Tang
title	Characterization of SARS-CoV-2 Variants N501Y.V1 and N501Y.V2 Spike on Viral Infectivity
title_short	Characterization of SARS-CoV-2 Variants N501Y.V1 and N501Y.V2 Spike on Viral Infectivity
title_full	Characterization of SARS-CoV-2 Variants N501Y.V1 and N501Y.V2 Spike on Viral Infectivity
title_fullStr	Characterization of SARS-CoV-2 Variants N501Y.V1 and N501Y.V2 Spike on Viral Infectivity
title_full_unstemmed	Characterization of SARS-CoV-2 Variants N501Y.V1 and N501Y.V2 Spike on Viral Infectivity
title_sort	characterization of sars-cov-2 variants n501y.v1 and n501y.v2 spike on viral infectivity
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3fa689bba3a2489e91661625c168c93b
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Characterization of SARS-CoV-2 Variants N501Y.V1 and N501Y.V2 Spike on Viral Infectivity

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