Mesoscopic cortical network reorganization during recovery of optic nerve injury in GCaMP6s mice

Abstract As the residual vision following a traumatic optic nerve injury can spontaneously recover over time, we explored the spontaneous plasticity of cortical networks during the early post-optic nerve crush (ONC) phase. Using in vivo wide-field calcium imaging on awake Thy1-GCaMP6s mice, we chara...

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Autores principales:	Marianne Groleau, Mojtaba Nazari-Ahangarkolaee, Matthieu P. Vanni, Jacqueline L. Higgins, Anne-Sophie Vézina Bédard, Bernhard A. Sabel, Majid H. Mohajerani, Elvire Vaucher
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3facd673d5f547bcba645f1cdd091aba
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spelling	oai:doaj.org-article:3facd673d5f547bcba645f1cdd091aba2021-12-02T15:11:50ZMesoscopic cortical network reorganization during recovery of optic nerve injury in GCaMP6s mice10.1038/s41598-020-78491-z2045-2322https://doaj.org/article/3facd673d5f547bcba645f1cdd091aba2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78491-zhttps://doaj.org/toc/2045-2322Abstract As the residual vision following a traumatic optic nerve injury can spontaneously recover over time, we explored the spontaneous plasticity of cortical networks during the early post-optic nerve crush (ONC) phase. Using in vivo wide-field calcium imaging on awake Thy1-GCaMP6s mice, we characterized resting state and evoked cortical activity before, during, and 31 days after ONC. The recovery of monocular visual acuity and depth perception was evaluated in parallel. Cortical responses to an LED flash decreased in the contralateral hemisphere in the primary visual cortex and in the secondary visual areas following the ONC, but was partially rescued between 3 and 5 days post-ONC, remaining stable thereafter. The connectivity between visual and non-visual regions was disorganized after the crush, as shown by a decorrelation, but correlated activity was restored 31 days after the injury. The number of surviving retinal ganglion cells dramatically dropped and remained low. At the behavioral level, the ONC resulted in visual acuity loss on the injured side and an increase in visual acuity with the non-injured eye. In conclusion, our results show a reorganization of connectivity between visual and associative cortical areas after an ONC, which is indicative of spontaneous cortical plasticity.Marianne GroleauMojtaba Nazari-AhangarkolaeeMatthieu P. VanniJacqueline L. HigginsAnne-Sophie Vézina BédardBernhard A. SabelMajid H. MohajeraniElvire VaucherNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marianne Groleau Mojtaba Nazari-Ahangarkolaee Matthieu P. Vanni Jacqueline L. Higgins Anne-Sophie Vézina Bédard Bernhard A. Sabel Majid H. Mohajerani Elvire Vaucher Mesoscopic cortical network reorganization during recovery of optic nerve injury in GCaMP6s mice
description	Abstract As the residual vision following a traumatic optic nerve injury can spontaneously recover over time, we explored the spontaneous plasticity of cortical networks during the early post-optic nerve crush (ONC) phase. Using in vivo wide-field calcium imaging on awake Thy1-GCaMP6s mice, we characterized resting state and evoked cortical activity before, during, and 31 days after ONC. The recovery of monocular visual acuity and depth perception was evaluated in parallel. Cortical responses to an LED flash decreased in the contralateral hemisphere in the primary visual cortex and in the secondary visual areas following the ONC, but was partially rescued between 3 and 5 days post-ONC, remaining stable thereafter. The connectivity between visual and non-visual regions was disorganized after the crush, as shown by a decorrelation, but correlated activity was restored 31 days after the injury. The number of surviving retinal ganglion cells dramatically dropped and remained low. At the behavioral level, the ONC resulted in visual acuity loss on the injured side and an increase in visual acuity with the non-injured eye. In conclusion, our results show a reorganization of connectivity between visual and associative cortical areas after an ONC, which is indicative of spontaneous cortical plasticity.
format	article
author	Marianne Groleau Mojtaba Nazari-Ahangarkolaee Matthieu P. Vanni Jacqueline L. Higgins Anne-Sophie Vézina Bédard Bernhard A. Sabel Majid H. Mohajerani Elvire Vaucher
author_facet	Marianne Groleau Mojtaba Nazari-Ahangarkolaee Matthieu P. Vanni Jacqueline L. Higgins Anne-Sophie Vézina Bédard Bernhard A. Sabel Majid H. Mohajerani Elvire Vaucher
author_sort	Marianne Groleau
title	Mesoscopic cortical network reorganization during recovery of optic nerve injury in GCaMP6s mice
title_short	Mesoscopic cortical network reorganization during recovery of optic nerve injury in GCaMP6s mice
title_full	Mesoscopic cortical network reorganization during recovery of optic nerve injury in GCaMP6s mice
title_fullStr	Mesoscopic cortical network reorganization during recovery of optic nerve injury in GCaMP6s mice
title_full_unstemmed	Mesoscopic cortical network reorganization during recovery of optic nerve injury in GCaMP6s mice
title_sort	mesoscopic cortical network reorganization during recovery of optic nerve injury in gcamp6s mice
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/3facd673d5f547bcba645f1cdd091aba
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Mesoscopic cortical network reorganization during recovery of optic nerve injury in GCaMP6s mice

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