Integrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model

Abstract Glioblastoma is a highly aggressive brain tumor, very invasive and thus difficult to eradicate with standard oncology therapies. Bioelectric treatments based on pulsed electric fields have proven to be a successful method to treat cancerous tissues. However, they rely on stiff electrodes, w...

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Autores principales:	Marie C. Lefevre, Gerwin Dijk, Attila Kaszas, Martin Baca, David Moreau, Rodney P. O’Connor
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Electronics TK7800-8360 Materials of engineering and construction. Mechanics of materials TA401-492
Acceso en línea:	https://doaj.org/article/3fad1231eb494978a2076513f152f2be
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spelling	oai:doaj.org-article:3fad1231eb494978a2076513f152f2be2021-12-02T19:06:36ZIntegrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model10.1038/s41528-021-00115-x2397-4621https://doaj.org/article/3fad1231eb494978a2076513f152f2be2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41528-021-00115-xhttps://doaj.org/toc/2397-4621Abstract Glioblastoma is a highly aggressive brain tumor, very invasive and thus difficult to eradicate with standard oncology therapies. Bioelectric treatments based on pulsed electric fields have proven to be a successful method to treat cancerous tissues. However, they rely on stiff electrodes, which cause acute and chronic injuries, especially in soft tissues like the brain. Here we demonstrate the feasibility of delivering pulsed electric fields with flexible electronics using an in ovo vascularized tumor model. We show with fluorescence widefield and multiphoton microscopy that pulsed electric fields induce vasoconstriction of blood vessels and evoke calcium signals in vascularized glioblastoma spheroids stably expressing a genetically encoded fluorescence reporter. Simulations of the electric field delivery are compared with the measured influence of electric field effects on cell membrane integrity in exposed tumor cells. Our results confirm the feasibility of flexible electronics as a means of delivering intense pulsed electric fields to tumors in an intravital 3D vascularized model of human glioblastoma.Marie C. LefevreGerwin DijkAttila KaszasMartin BacaDavid MoreauRodney P. O’ConnorNature PortfolioarticleElectronicsTK7800-8360Materials of engineering and construction. Mechanics of materialsTA401-492ENnpj Flexible Electronics, Vol 5, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Electronics TK7800-8360 Materials of engineering and construction. Mechanics of materials TA401-492
spellingShingle	Electronics TK7800-8360 Materials of engineering and construction. Mechanics of materials TA401-492 Marie C. Lefevre Gerwin Dijk Attila Kaszas Martin Baca David Moreau Rodney P. O’Connor Integrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model
description	Abstract Glioblastoma is a highly aggressive brain tumor, very invasive and thus difficult to eradicate with standard oncology therapies. Bioelectric treatments based on pulsed electric fields have proven to be a successful method to treat cancerous tissues. However, they rely on stiff electrodes, which cause acute and chronic injuries, especially in soft tissues like the brain. Here we demonstrate the feasibility of delivering pulsed electric fields with flexible electronics using an in ovo vascularized tumor model. We show with fluorescence widefield and multiphoton microscopy that pulsed electric fields induce vasoconstriction of blood vessels and evoke calcium signals in vascularized glioblastoma spheroids stably expressing a genetically encoded fluorescence reporter. Simulations of the electric field delivery are compared with the measured influence of electric field effects on cell membrane integrity in exposed tumor cells. Our results confirm the feasibility of flexible electronics as a means of delivering intense pulsed electric fields to tumors in an intravital 3D vascularized model of human glioblastoma.
format	article
author	Marie C. Lefevre Gerwin Dijk Attila Kaszas Martin Baca David Moreau Rodney P. O’Connor
author_facet	Marie C. Lefevre Gerwin Dijk Attila Kaszas Martin Baca David Moreau Rodney P. O’Connor
author_sort	Marie C. Lefevre
title	Integrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model
title_short	Integrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model
title_full	Integrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model
title_fullStr	Integrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model
title_full_unstemmed	Integrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model
title_sort	integrating flexible electronics for pulsed electric field delivery in a vascularized 3d glioblastoma model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3fad1231eb494978a2076513f152f2be
work_keys_str_mv	AT marieclefevre integratingflexibleelectronicsforpulsedelectricfielddeliveryinavascularized3dglioblastomamodel AT gerwindijk integratingflexibleelectronicsforpulsedelectricfielddeliveryinavascularized3dglioblastomamodel AT attilakaszas integratingflexibleelectronicsforpulsedelectricfielddeliveryinavascularized3dglioblastomamodel AT martinbaca integratingflexibleelectronicsforpulsedelectricfielddeliveryinavascularized3dglioblastomamodel AT davidmoreau integratingflexibleelectronicsforpulsedelectricfielddeliveryinavascularized3dglioblastomamodel AT rodneypoconnor integratingflexibleelectronicsforpulsedelectricfielddeliveryinavascularized3dglioblastomamodel
_version_	1718377143430283264

Integrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model

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