The Epstein-Barr Virus BRRF1 Gene Is Dispensable for Viral Replication in HEK293 cells and Transformation

Abstract The Epstein-Barr virus (EBV) is a gamma-herpesvirus associated with several malignancies. It establishes a latent infection in B lymphocytes and is occasionally reactivated to enter the lytic cycle. Here we examined the role of the EBV gene BRRF1, which is expressed in the lytic state. We f...

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Autores principales:	Masahiro Yoshida, Takahiro Watanabe, Yohei Narita, Yoshitaka Sato, Fumi Goshima, Hiroshi Kimura, Takayuki Murata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3fb426ac40bb420f8702ee17bcf0ea01
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spelling	oai:doaj.org-article:3fb426ac40bb420f8702ee17bcf0ea012021-12-02T16:06:35ZThe Epstein-Barr Virus BRRF1 Gene Is Dispensable for Viral Replication in HEK293 cells and Transformation10.1038/s41598-017-06413-72045-2322https://doaj.org/article/3fb426ac40bb420f8702ee17bcf0ea012017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06413-7https://doaj.org/toc/2045-2322Abstract The Epstein-Barr virus (EBV) is a gamma-herpesvirus associated with several malignancies. It establishes a latent infection in B lymphocytes and is occasionally reactivated to enter the lytic cycle. Here we examined the role of the EBV gene BRRF1, which is expressed in the lytic state. We first confirmed, using a DNA polymerase inhibitor, that the BRRF1 gene is expressed with early kinetics. A BRRF1-deficient recombinant virus was constructed using a bacterial artificial chromosome system. No obvious differences were observed between the wild-type, BRRF1-deficient mutant and the revertant virus in HEK293 cells in terms of viral lytic protein expression, viral DNA synthesis, progeny production, pre-latent abortive lytic gene expression and transformation of primary B cells. However, reporter assays indicated that BRRF1 may activate transcription in promoter- and cell type-dependent manners. Taken together, BRRF1 is dispensable for viral replication in HEK293 cells and transformation of B cells, but it may have effects on transcription.Masahiro YoshidaTakahiro WatanabeYohei NaritaYoshitaka SatoFumi GoshimaHiroshi KimuraTakayuki MurataNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masahiro Yoshida Takahiro Watanabe Yohei Narita Yoshitaka Sato Fumi Goshima Hiroshi Kimura Takayuki Murata The Epstein-Barr Virus BRRF1 Gene Is Dispensable for Viral Replication in HEK293 cells and Transformation
description	Abstract The Epstein-Barr virus (EBV) is a gamma-herpesvirus associated with several malignancies. It establishes a latent infection in B lymphocytes and is occasionally reactivated to enter the lytic cycle. Here we examined the role of the EBV gene BRRF1, which is expressed in the lytic state. We first confirmed, using a DNA polymerase inhibitor, that the BRRF1 gene is expressed with early kinetics. A BRRF1-deficient recombinant virus was constructed using a bacterial artificial chromosome system. No obvious differences were observed between the wild-type, BRRF1-deficient mutant and the revertant virus in HEK293 cells in terms of viral lytic protein expression, viral DNA synthesis, progeny production, pre-latent abortive lytic gene expression and transformation of primary B cells. However, reporter assays indicated that BRRF1 may activate transcription in promoter- and cell type-dependent manners. Taken together, BRRF1 is dispensable for viral replication in HEK293 cells and transformation of B cells, but it may have effects on transcription.
format	article
author	Masahiro Yoshida Takahiro Watanabe Yohei Narita Yoshitaka Sato Fumi Goshima Hiroshi Kimura Takayuki Murata
author_facet	Masahiro Yoshida Takahiro Watanabe Yohei Narita Yoshitaka Sato Fumi Goshima Hiroshi Kimura Takayuki Murata
author_sort	Masahiro Yoshida
title	The Epstein-Barr Virus BRRF1 Gene Is Dispensable for Viral Replication in HEK293 cells and Transformation
title_short	The Epstein-Barr Virus BRRF1 Gene Is Dispensable for Viral Replication in HEK293 cells and Transformation
title_full	The Epstein-Barr Virus BRRF1 Gene Is Dispensable for Viral Replication in HEK293 cells and Transformation
title_fullStr	The Epstein-Barr Virus BRRF1 Gene Is Dispensable for Viral Replication in HEK293 cells and Transformation
title_full_unstemmed	The Epstein-Barr Virus BRRF1 Gene Is Dispensable for Viral Replication in HEK293 cells and Transformation
title_sort	epstein-barr virus brrf1 gene is dispensable for viral replication in hek293 cells and transformation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3fb426ac40bb420f8702ee17bcf0ea01
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The Epstein-Barr Virus BRRF1 Gene Is Dispensable for Viral Replication in HEK293 cells and Transformation

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