Molecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia

Abstract. Fachrial E, Putri RRJS, Lister INE, Anggraini S, Harmileni, Nugroho TT, Saryono. 2020. Molecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia. Biodiversitas 21: 4719-47...

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Autores principales:	Edy Fachrial, Raden Roro Jenny Satyo Putri, I Nyoman Ehrich Lister, Sari Anggraini, Harmileni HARMILENI, Titania T. Nugroho, Saryono SARYONO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	cellulase, geothermal hot springs, protease, thermophilic bacteria Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3fc0475dcb3b43b2a0f852be94ccf57e
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spelling	oai:doaj.org-article:3fc0475dcb3b43b2a0f852be94ccf57e2021-11-22T00:51:09ZMolecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia1412-033X2085-472210.13057/biodiv/d211035https://doaj.org/article/3fc0475dcb3b43b2a0f852be94ccf57e2020-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6476https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Fachrial E, Putri RRJS, Lister INE, Anggraini S, Harmileni, Nugroho TT, Saryono. 2020. Molecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia. Biodiversitas 21: 4719-4725. The industrial need for stable microbial enzymes tends to increase every year. The aim of this study was to isolate and identify the protease and cellulase producing thermophilic bacteria isolated from the geothermal spring of Lau Sidebuk Debuk, North Sumatra, Indonesia. The protease activity was determined based on the formation of halo zone on Skim Milk Agar, while cellulase was confirmed using CMC plate agar method. Nine bacterial isolates were successfully obtained from the sediments and water, and both activities were observed in only one isolate, known as UTMSA14. These isolates were characterized biochemically and morphologically. The molecular identification of UTMSA14 was done by 16S rRNA sequencing based on BLAST and phylogenetic analysis using MEGA X. Phylogenetic analysis showed that UTMSA14 clustered together with Bacillus tequilensis strain 10b (accession number NR_104919.1), which ascertained the isolate as Bacillus tequilensis strain 10b (accession number NR_104919.1). This is the first report of a thermophilic bacteria isolated from Lau Sidebuk Debuk hot springs, with the ability to produce cellulase and protease. further research is needed to purify and characterize the resulting enzymes, with a potential for industrial applications.Edy FachrialRaden Roro Jenny Satyo PutriI Nyoman Ehrich ListerSari AnggrainiHarmileni HARMILENITitania T. NugrohoSaryono SARYONOMBI & UNS Soloarticlecellulase, geothermal hot springs, protease, thermophilic bacteriaBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cellulase, geothermal hot springs, protease, thermophilic bacteria Biology (General) QH301-705.5
spellingShingle	cellulase, geothermal hot springs, protease, thermophilic bacteria Biology (General) QH301-705.5 Edy Fachrial Raden Roro Jenny Satyo Putri I Nyoman Ehrich Lister Sari Anggraini Harmileni HARMILENI Titania T. Nugroho Saryono SARYONO Molecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia
description	Abstract. Fachrial E, Putri RRJS, Lister INE, Anggraini S, Harmileni, Nugroho TT, Saryono. 2020. Molecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia. Biodiversitas 21: 4719-4725. The industrial need for stable microbial enzymes tends to increase every year. The aim of this study was to isolate and identify the protease and cellulase producing thermophilic bacteria isolated from the geothermal spring of Lau Sidebuk Debuk, North Sumatra, Indonesia. The protease activity was determined based on the formation of halo zone on Skim Milk Agar, while cellulase was confirmed using CMC plate agar method. Nine bacterial isolates were successfully obtained from the sediments and water, and both activities were observed in only one isolate, known as UTMSA14. These isolates were characterized biochemically and morphologically. The molecular identification of UTMSA14 was done by 16S rRNA sequencing based on BLAST and phylogenetic analysis using MEGA X. Phylogenetic analysis showed that UTMSA14 clustered together with Bacillus tequilensis strain 10b (accession number NR_104919.1), which ascertained the isolate as Bacillus tequilensis strain 10b (accession number NR_104919.1). This is the first report of a thermophilic bacteria isolated from Lau Sidebuk Debuk hot springs, with the ability to produce cellulase and protease. further research is needed to purify and characterize the resulting enzymes, with a potential for industrial applications.
format	article
author	Edy Fachrial Raden Roro Jenny Satyo Putri I Nyoman Ehrich Lister Sari Anggraini Harmileni HARMILENI Titania T. Nugroho Saryono SARYONO
author_facet	Edy Fachrial Raden Roro Jenny Satyo Putri I Nyoman Ehrich Lister Sari Anggraini Harmileni HARMILENI Titania T. Nugroho Saryono SARYONO
author_sort	Edy Fachrial
title	Molecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia
title_short	Molecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia
title_full	Molecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia
title_fullStr	Molecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia
title_full_unstemmed	Molecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia
title_sort	molecular identification of cellulase and protease producing bacillus tequilensis utmsa14 isolated from the geothermal hot spring in lau sidebuk debuk, north sumatra, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/3fc0475dcb3b43b2a0f852be94ccf57e
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Molecular identification of cellulase and protease producing Bacillus tequilensis UTMSA14 isolated from the geothermal hot spring in Lau Sidebuk Debuk, North Sumatra, Indonesia

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