A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues

Testing mechanical forces on native molecules in natural environments remains a challenge. Here the authors engineer titin to carry a HaloTag-TEV insertion to allow analysis of dynamics under force in muscle fibers.

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Detalles Bibliográficos
Autores principales: Jaime Andrés Rivas-Pardo, Yong Li, Zsolt Mártonfalvi, Rafael Tapia-Rojo, Andreas Unger, Ángel Fernández-Trasancos, Elías Herrero-Galán, Diana Velázquez-Carreras, Julio M. Fernández, Wolfgang A. Linke, Jorge Alegre-Cebollada
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/3fc2e404086144d88118c34e7a917844
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Descripción
Sumario:Testing mechanical forces on native molecules in natural environments remains a challenge. Here the authors engineer titin to carry a HaloTag-TEV insertion to allow analysis of dynamics under force in muscle fibers.