A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues

Testing mechanical forces on native molecules in natural environments remains a challenge. Here the authors engineer titin to carry a HaloTag-TEV insertion to allow analysis of dynamics under force in muscle fibers.

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Detalles Bibliográficos
Autores principales:	Jaime Andrés Rivas-Pardo, Yong Li, Zsolt Mártonfalvi, Rafael Tapia-Rojo, Andreas Unger, Ángel Fernández-Trasancos, Elías Herrero-Galán, Diana Velázquez-Carreras, Julio M. Fernández, Wolfgang A. Linke, Jorge Alegre-Cebollada
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/3fc2e404086144d88118c34e7a917844
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spelling	oai:doaj.org-article:3fc2e404086144d88118c34e7a9178442021-12-02T15:37:14ZA HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues10.1038/s41467-020-15465-92041-1723https://doaj.org/article/3fc2e404086144d88118c34e7a9178442020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15465-9https://doaj.org/toc/2041-1723Testing mechanical forces on native molecules in natural environments remains a challenge. Here the authors engineer titin to carry a HaloTag-TEV insertion to allow analysis of dynamics under force in muscle fibers.Jaime Andrés Rivas-PardoYong LiZsolt MártonfalviRafael Tapia-RojoAndreas UngerÁngel Fernández-TrasancosElías Herrero-GalánDiana Velázquez-CarrerasJulio M. FernándezWolfgang A. LinkeJorge Alegre-CebolladaNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jaime Andrés Rivas-Pardo Yong Li Zsolt Mártonfalvi Rafael Tapia-Rojo Andreas Unger Ángel Fernández-Trasancos Elías Herrero-Galán Diana Velázquez-Carreras Julio M. Fernández Wolfgang A. Linke Jorge Alegre-Cebollada A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues
description	Testing mechanical forces on native molecules in natural environments remains a challenge. Here the authors engineer titin to carry a HaloTag-TEV insertion to allow analysis of dynamics under force in muscle fibers.
format	article
author	Jaime Andrés Rivas-Pardo Yong Li Zsolt Mártonfalvi Rafael Tapia-Rojo Andreas Unger Ángel Fernández-Trasancos Elías Herrero-Galán Diana Velázquez-Carreras Julio M. Fernández Wolfgang A. Linke Jorge Alegre-Cebollada
author_facet	Jaime Andrés Rivas-Pardo Yong Li Zsolt Mártonfalvi Rafael Tapia-Rojo Andreas Unger Ángel Fernández-Trasancos Elías Herrero-Galán Diana Velázquez-Carreras Julio M. Fernández Wolfgang A. Linke Jorge Alegre-Cebollada
author_sort	Jaime Andrés Rivas-Pardo
title	A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues
title_short	A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues
title_full	A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues
title_fullStr	A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues
title_full_unstemmed	A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues
title_sort	halotag-tev genetic cassette for mechanical phenotyping of proteins from tissues
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/3fc2e404086144d88118c34e7a917844
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A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues

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