Transcriptome divergence between developmental senescence and premature senescence in Nicotiana tabacum L.

Abstract Senescence is a degenerative process triggered by intricate and coordinated regulatory networks, and the mechanisms of age-dependent senescence and stress-induced premature senescence still remain largely elusive. Thus we selected leaf samples of developmental senescence (DS) and premature...

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Autores principales:	Zhe Zhao, Jia-Wen Zhang, Shao-Hao Lu, Hong Zhang, Fang Liu, Bo Fu, Ming-Qin Zhao, Hui Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3fc8a3328db749dd8eb97b0a29cbfcf3
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spelling	oai:doaj.org-article:3fc8a3328db749dd8eb97b0a29cbfcf32021-12-02T15:10:17ZTranscriptome divergence between developmental senescence and premature senescence in Nicotiana tabacum L.10.1038/s41598-020-77395-22045-2322https://doaj.org/article/3fc8a3328db749dd8eb97b0a29cbfcf32020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77395-2https://doaj.org/toc/2045-2322Abstract Senescence is a degenerative process triggered by intricate and coordinated regulatory networks, and the mechanisms of age-dependent senescence and stress-induced premature senescence still remain largely elusive. Thus we selected leaf samples of developmental senescence (DS) and premature senescence (PS) to reveal the regulatory divergence. Senescent leaves were confirmed by yellowing symptom and physiological measurement. A total of 1171 and 309 genes (DEGs) were significantly expressed respectively in the whole process of DS and PS. Up-regulated DEGs in PS were mostly related to ion transport, while the down-regulated DEGs were mainly associated with oxidoreductase activity and sesquiterpenoid and triterpenoid biosynthesis. In DS, photosynthesis, precursor metabolites and energy, protein processing in endoplasmic reticulum, flavonoid biosynthesis were notable. Moreover, we found the vital pathways shared by DS and PS, of which the DEGs were analyzed further via protein–protein interaction (PPI) network analysis to explore the alteration responding to two types of senescence. In addition, plant hormone transduction pathway was mapped by related DEGs, suggesting that ABA and ethylene signaling played pivotal roles in formulating the distinction of DS and PS. Finally, we conducted a model containing oxidative stress and ABA signaling as two hub points, which highlighted the major difference and predicted the possible mechanism under DS and PS. This work gained new insight into molecular divergence of developmental senescence and premature senescence and would provide reference on potential mechanism initiating and motivating senescence for further study.Zhe ZhaoJia-Wen ZhangShao-Hao LuHong ZhangFang LiuBo FuMing-Qin ZhaoHui LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhe Zhao Jia-Wen Zhang Shao-Hao Lu Hong Zhang Fang Liu Bo Fu Ming-Qin Zhao Hui Liu Transcriptome divergence between developmental senescence and premature senescence in Nicotiana tabacum L.
description	Abstract Senescence is a degenerative process triggered by intricate and coordinated regulatory networks, and the mechanisms of age-dependent senescence and stress-induced premature senescence still remain largely elusive. Thus we selected leaf samples of developmental senescence (DS) and premature senescence (PS) to reveal the regulatory divergence. Senescent leaves were confirmed by yellowing symptom and physiological measurement. A total of 1171 and 309 genes (DEGs) were significantly expressed respectively in the whole process of DS and PS. Up-regulated DEGs in PS were mostly related to ion transport, while the down-regulated DEGs were mainly associated with oxidoreductase activity and sesquiterpenoid and triterpenoid biosynthesis. In DS, photosynthesis, precursor metabolites and energy, protein processing in endoplasmic reticulum, flavonoid biosynthesis were notable. Moreover, we found the vital pathways shared by DS and PS, of which the DEGs were analyzed further via protein–protein interaction (PPI) network analysis to explore the alteration responding to two types of senescence. In addition, plant hormone transduction pathway was mapped by related DEGs, suggesting that ABA and ethylene signaling played pivotal roles in formulating the distinction of DS and PS. Finally, we conducted a model containing oxidative stress and ABA signaling as two hub points, which highlighted the major difference and predicted the possible mechanism under DS and PS. This work gained new insight into molecular divergence of developmental senescence and premature senescence and would provide reference on potential mechanism initiating and motivating senescence for further study.
format	article
author	Zhe Zhao Jia-Wen Zhang Shao-Hao Lu Hong Zhang Fang Liu Bo Fu Ming-Qin Zhao Hui Liu
author_facet	Zhe Zhao Jia-Wen Zhang Shao-Hao Lu Hong Zhang Fang Liu Bo Fu Ming-Qin Zhao Hui Liu
author_sort	Zhe Zhao
title	Transcriptome divergence between developmental senescence and premature senescence in Nicotiana tabacum L.
title_short	Transcriptome divergence between developmental senescence and premature senescence in Nicotiana tabacum L.
title_full	Transcriptome divergence between developmental senescence and premature senescence in Nicotiana tabacum L.
title_fullStr	Transcriptome divergence between developmental senescence and premature senescence in Nicotiana tabacum L.
title_full_unstemmed	Transcriptome divergence between developmental senescence and premature senescence in Nicotiana tabacum L.
title_sort	transcriptome divergence between developmental senescence and premature senescence in nicotiana tabacum l.
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/3fc8a3328db749dd8eb97b0a29cbfcf3
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Transcriptome divergence between developmental senescence and premature senescence in Nicotiana tabacum L.

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