Development of a novel real‐time PCR assay targeting p54 gene for rapid detection of African swine fever virus (ASFV) strains circulating in Vietnam

Abstract African swine fever (ASF) continues to cause outbreaks throughout regions of Africa, Europe and Asia. The disease can cause severe morbidity and mortality resulting in serious economic losses. Since there is no vaccine available to control ASF, early detection is critical to contain and con...

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Autores principales:	Thi Bich Ngoc Trinh, Thang Truong, Van Tam Nguyen, Xuan Dang Vu, Le Anh Dao, Thi Lan Nguyen, Aruna Ambagala, Shawn Babiuk, Jinsik Oh, Daesub Song, Van Phan Le
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	African swine fever real‐time PCR VNUA‐p54 Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/3fcaf302fffe4f309eb65824c98644a1
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spelling	oai:doaj.org-article:3fcaf302fffe4f309eb65824c98644a12021-11-19T17:14:25ZDevelopment of a novel real‐time PCR assay targeting p54 gene for rapid detection of African swine fever virus (ASFV) strains circulating in Vietnam2053-109510.1002/vms3.605https://doaj.org/article/3fcaf302fffe4f309eb65824c98644a12021-11-01T00:00:00Zhttps://doi.org/10.1002/vms3.605https://doaj.org/toc/2053-1095Abstract African swine fever (ASF) continues to cause outbreaks throughout regions of Africa, Europe and Asia. The disease can cause severe morbidity and mortality resulting in serious economic losses. Since there is no vaccine available to control ASF, early detection is critical to contain and control the disease. The aim of this study was to develop a novel real‐time PCR assay based on highly conserved ASFV gene E183L (p54). The limit of detection of the assay, VNUA‐p54 real‐time PCR, was 2.63 copies/reaction and 2 Log10 HAD50/ml. The VNUA‐p54 real‐time PCR was able to detect fifteen different ASFV reference strains representing p72 genotypes I, II and V. The assay was specific and did not amplify other swine viruses including CSFV, FMDV, PRRSV and PEDV. The diagnostic sensitivity of the real‐time PCR assay was evaluated using 200 field clinical specimens collected from swine farms located in different provinces in Vietnam. The VNUA‐p54 real‐time PCR assay is an additional tool for ASF diagnostics and can be used in combination with other p72 based ASFV real‐time PCR assays as a rapid confirmatory assay.Thi Bich Ngoc TrinhThang TruongVan Tam NguyenXuan Dang VuLe Anh DaoThi Lan NguyenAruna AmbagalaShawn BabiukJinsik OhDaesub SongVan Phan LeWileyarticleAfrican swine feverreal‐time PCRVNUA‐p54Veterinary medicineSF600-1100ENVeterinary Medicine and Science, Vol 7, Iss 6, Pp 2268-2272 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	African swine fever real‐time PCR VNUA‐p54 Veterinary medicine SF600-1100
spellingShingle	African swine fever real‐time PCR VNUA‐p54 Veterinary medicine SF600-1100 Thi Bich Ngoc Trinh Thang Truong Van Tam Nguyen Xuan Dang Vu Le Anh Dao Thi Lan Nguyen Aruna Ambagala Shawn Babiuk Jinsik Oh Daesub Song Van Phan Le Development of a novel real‐time PCR assay targeting p54 gene for rapid detection of African swine fever virus (ASFV) strains circulating in Vietnam
description	Abstract African swine fever (ASF) continues to cause outbreaks throughout regions of Africa, Europe and Asia. The disease can cause severe morbidity and mortality resulting in serious economic losses. Since there is no vaccine available to control ASF, early detection is critical to contain and control the disease. The aim of this study was to develop a novel real‐time PCR assay based on highly conserved ASFV gene E183L (p54). The limit of detection of the assay, VNUA‐p54 real‐time PCR, was 2.63 copies/reaction and 2 Log10 HAD50/ml. The VNUA‐p54 real‐time PCR was able to detect fifteen different ASFV reference strains representing p72 genotypes I, II and V. The assay was specific and did not amplify other swine viruses including CSFV, FMDV, PRRSV and PEDV. The diagnostic sensitivity of the real‐time PCR assay was evaluated using 200 field clinical specimens collected from swine farms located in different provinces in Vietnam. The VNUA‐p54 real‐time PCR assay is an additional tool for ASF diagnostics and can be used in combination with other p72 based ASFV real‐time PCR assays as a rapid confirmatory assay.
format	article
author	Thi Bich Ngoc Trinh Thang Truong Van Tam Nguyen Xuan Dang Vu Le Anh Dao Thi Lan Nguyen Aruna Ambagala Shawn Babiuk Jinsik Oh Daesub Song Van Phan Le
author_facet	Thi Bich Ngoc Trinh Thang Truong Van Tam Nguyen Xuan Dang Vu Le Anh Dao Thi Lan Nguyen Aruna Ambagala Shawn Babiuk Jinsik Oh Daesub Song Van Phan Le
author_sort	Thi Bich Ngoc Trinh
title	Development of a novel real‐time PCR assay targeting p54 gene for rapid detection of African swine fever virus (ASFV) strains circulating in Vietnam
title_short	Development of a novel real‐time PCR assay targeting p54 gene for rapid detection of African swine fever virus (ASFV) strains circulating in Vietnam
title_full	Development of a novel real‐time PCR assay targeting p54 gene for rapid detection of African swine fever virus (ASFV) strains circulating in Vietnam
title_fullStr	Development of a novel real‐time PCR assay targeting p54 gene for rapid detection of African swine fever virus (ASFV) strains circulating in Vietnam
title_full_unstemmed	Development of a novel real‐time PCR assay targeting p54 gene for rapid detection of African swine fever virus (ASFV) strains circulating in Vietnam
title_sort	development of a novel real‐time pcr assay targeting p54 gene for rapid detection of african swine fever virus (asfv) strains circulating in vietnam
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/3fcaf302fffe4f309eb65824c98644a1
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Development of a novel real‐time PCR assay targeting p54 gene for rapid detection of African swine fever virus (ASFV) strains circulating in Vietnam

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