Clinicopathological significance of the EMT-related proteins and their interrelationships in prostate cancer. An immunohistochemical study.

The chronic inflammation influences a microenvironment, where as a result of losing control over tissue homeostatic mechanisms, the carcinogenesis process may be induced. Inflammatory response cells can secrete a number of factors that support both initiation and progression of cancer and also they...

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Autores principales:	Martyna Parol-Kulczyk, Arkadiusz Gzil, Joanna Maciejewska, Magdalena Bodnar, Dariusz Grzanka
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3fce8d7a62ef459ea41cee24e248f7da
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spelling	oai:doaj.org-article:3fce8d7a62ef459ea41cee24e248f7da2021-12-02T20:15:47ZClinicopathological significance of the EMT-related proteins and their interrelationships in prostate cancer. An immunohistochemical study.1932-620310.1371/journal.pone.0253112https://doaj.org/article/3fce8d7a62ef459ea41cee24e248f7da2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253112https://doaj.org/toc/1932-6203The chronic inflammation influences a microenvironment, where as a result of losing control over tissue homeostatic mechanisms, the carcinogenesis process may be induced. Inflammatory response cells can secrete a number of factors that support both initiation and progression of cancer and also they may consequently induct an epithelial-mesenchymal transition (EMT), the process responsible for development of distant metastasis. Macrophage migration inhibitory factor (MIF) acts as a pro-inflammatory cytokine that is considered as a link between chronic inflammation and tumor development. MIF can function as a modulator of important cancer-related genes expression, as well as an activator of signaling pathways that promotes the development of prostate cancer. The study was performed on FFPE tissues resected from patients who underwent radical prostatectomy. To investigate the relationship of studied proteins with involvement in tumor progression and initiation of epithelial-to-mesenchymal transition (EMT) process, we selected clinicopathological parameters related to tumor progression. Immunohistochemical analyses of MIF, SOX-4, β-catenin and E-cadherin were performed on TMA slides. We found a statistically significant correlation of overall β-catenin expression with the both lymph node metastasis (p<0.001) and presence of angioinvasion (p = 0.012). Membrane β-catenin expression was associated with distant metastasis (p = 0.021). In turn, nuclear MIF was correlated with lymph node metastasis (p = 0.003). The positive protein-protein correlations have been shown between the total β-catenin protein expression level with level of nuclear SOX-4 protein expression (r = 0.27; p<0.05) as well as negative correlation of β-catenin expression with level of nuclear MIF protein expression (r = -0.23; p<0.05). Our results seem promising and strongly highlight the potential role of MIF in development of nodal metastases as well as may confirm an involvement of β-catenin in disease spread in case of prostate cancer.Martyna Parol-KulczykArkadiusz GzilJoanna MaciejewskaMagdalena BodnarDariusz GrzankaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253112 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martyna Parol-Kulczyk Arkadiusz Gzil Joanna Maciejewska Magdalena Bodnar Dariusz Grzanka Clinicopathological significance of the EMT-related proteins and their interrelationships in prostate cancer. An immunohistochemical study.
description	The chronic inflammation influences a microenvironment, where as a result of losing control over tissue homeostatic mechanisms, the carcinogenesis process may be induced. Inflammatory response cells can secrete a number of factors that support both initiation and progression of cancer and also they may consequently induct an epithelial-mesenchymal transition (EMT), the process responsible for development of distant metastasis. Macrophage migration inhibitory factor (MIF) acts as a pro-inflammatory cytokine that is considered as a link between chronic inflammation and tumor development. MIF can function as a modulator of important cancer-related genes expression, as well as an activator of signaling pathways that promotes the development of prostate cancer. The study was performed on FFPE tissues resected from patients who underwent radical prostatectomy. To investigate the relationship of studied proteins with involvement in tumor progression and initiation of epithelial-to-mesenchymal transition (EMT) process, we selected clinicopathological parameters related to tumor progression. Immunohistochemical analyses of MIF, SOX-4, β-catenin and E-cadherin were performed on TMA slides. We found a statistically significant correlation of overall β-catenin expression with the both lymph node metastasis (p<0.001) and presence of angioinvasion (p = 0.012). Membrane β-catenin expression was associated with distant metastasis (p = 0.021). In turn, nuclear MIF was correlated with lymph node metastasis (p = 0.003). The positive protein-protein correlations have been shown between the total β-catenin protein expression level with level of nuclear SOX-4 protein expression (r = 0.27; p<0.05) as well as negative correlation of β-catenin expression with level of nuclear MIF protein expression (r = -0.23; p<0.05). Our results seem promising and strongly highlight the potential role of MIF in development of nodal metastases as well as may confirm an involvement of β-catenin in disease spread in case of prostate cancer.
format	article
author	Martyna Parol-Kulczyk Arkadiusz Gzil Joanna Maciejewska Magdalena Bodnar Dariusz Grzanka
author_facet	Martyna Parol-Kulczyk Arkadiusz Gzil Joanna Maciejewska Magdalena Bodnar Dariusz Grzanka
author_sort	Martyna Parol-Kulczyk
title	Clinicopathological significance of the EMT-related proteins and their interrelationships in prostate cancer. An immunohistochemical study.
title_short	Clinicopathological significance of the EMT-related proteins and their interrelationships in prostate cancer. An immunohistochemical study.
title_full	Clinicopathological significance of the EMT-related proteins and their interrelationships in prostate cancer. An immunohistochemical study.
title_fullStr	Clinicopathological significance of the EMT-related proteins and their interrelationships in prostate cancer. An immunohistochemical study.
title_full_unstemmed	Clinicopathological significance of the EMT-related proteins and their interrelationships in prostate cancer. An immunohistochemical study.
title_sort	clinicopathological significance of the emt-related proteins and their interrelationships in prostate cancer. an immunohistochemical study.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3fce8d7a62ef459ea41cee24e248f7da
work_keys_str_mv	AT martynaparolkulczyk clinicopathologicalsignificanceoftheemtrelatedproteinsandtheirinterrelationshipsinprostatecanceranimmunohistochemicalstudy AT arkadiuszgzil clinicopathologicalsignificanceoftheemtrelatedproteinsandtheirinterrelationshipsinprostatecanceranimmunohistochemicalstudy AT joannamaciejewska clinicopathologicalsignificanceoftheemtrelatedproteinsandtheirinterrelationshipsinprostatecanceranimmunohistochemicalstudy AT magdalenabodnar clinicopathologicalsignificanceoftheemtrelatedproteinsandtheirinterrelationshipsinprostatecanceranimmunohistochemicalstudy AT dariuszgrzanka clinicopathologicalsignificanceoftheemtrelatedproteinsandtheirinterrelationshipsinprostatecanceranimmunohistochemicalstudy
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Clinicopathological significance of the EMT-related proteins and their interrelationships in prostate cancer. An immunohistochemical study.

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