Cucumis sativus L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway

Cucumis sativus L. is globally cultivated as an edible vegetable. Besides its nutritional benefits, it is used in traditional medicines against various ailments. The current study was designed to elucidate the multi-target mechanisms of a C. sativus seeds extrac...

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Autores principales:	Muqeet Wahid, Fatima Saqib, Hanadi Talal Ahmedah, Claudia Mihaela Gavris, Vincenzo De Feo, Mircea Hogea, Marius Moga, Radu Chicea
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	asthma antidiarrheal bronchodilator <i>Cucumis sativus</i> cucumber HPLC Medicine R Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/3fd0f28cb90d4402a06f80387ca6c1ab
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spelling	oai:doaj.org-article:3fd0f28cb90d4402a06f80387ca6c1ab2021-11-25T18:40:09Z<i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway10.3390/ph141111971424-8247https://doaj.org/article/3fd0f28cb90d4402a06f80387ca6c1ab2021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1197https://doaj.org/toc/1424-8247<i>Cucumis sativus</i> L. is globally cultivated as an edible vegetable. Besides its nutritional benefits, it is used in traditional medicines against various ailments. The current study was designed to elucidate the multi-target mechanisms of a <i>C. sativus</i> seeds extract against asthma and diarrhea using network pharmacology along with a molecular docking approach. Furthermore, in-vitro and in-vivo experiments were conducted to verify the mechanistic insight of in silico studies. LC-ESI-MS/MS was performed to identify the bioactive compounds in the extract; later, some compounds were quantified by HPLC. <i>C. sativus</i> seed. EtOH has kaempferol in higher concentration 783.02 µg/g, followed by quercetin (693.83 µg/g) and luteolin (617.17 µg/g). In silico studies showed that bioactive compounds interfered with asthma and diarrhea-associated target genes, which are members of calcium-mediated signaling to exert a calcium channel blocker activity. The seeds extract exerted a concentration-dependent spasmolytic response on isolated jejunum, trachea, and urinary bladder preparations and caused relaxation of spastic contraction of K<sup>+</sup> (80 mM) with suppressed calcium concentration-response curves at dose 0.3 and 1 mg/mL. It also showed antiperistalsis, antidiarrheal and antisecretory activity in animal models. Thus, <i>C. sativus</i> seeds have therapeutic effects by regulating the contractile response through a calcium-mediated signaling pathway.Muqeet WahidFatima SaqibHanadi Talal AhmedahClaudia Mihaela GavrisVincenzo De FeoMircea HogeaMarius MogaRadu ChiceaMDPI AGarticleasthmaantidiarrhealbronchodilator<i>Cucumis sativus</i>cucumberHPLCMedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1197, p 1197 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	asthma antidiarrheal bronchodilator <i>Cucumis sativus</i> cucumber HPLC Medicine R Pharmacy and materia medica RS1-441
spellingShingle	asthma antidiarrheal bronchodilator <i>Cucumis sativus</i> cucumber HPLC Medicine R Pharmacy and materia medica RS1-441 Muqeet Wahid Fatima Saqib Hanadi Talal Ahmedah Claudia Mihaela Gavris Vincenzo De Feo Mircea Hogea Marius Moga Radu Chicea <i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway
description	<i>Cucumis sativus</i> L. is globally cultivated as an edible vegetable. Besides its nutritional benefits, it is used in traditional medicines against various ailments. The current study was designed to elucidate the multi-target mechanisms of a <i>C. sativus</i> seeds extract against asthma and diarrhea using network pharmacology along with a molecular docking approach. Furthermore, in-vitro and in-vivo experiments were conducted to verify the mechanistic insight of in silico studies. LC-ESI-MS/MS was performed to identify the bioactive compounds in the extract; later, some compounds were quantified by HPLC. <i>C. sativus</i> seed. EtOH has kaempferol in higher concentration 783.02 µg/g, followed by quercetin (693.83 µg/g) and luteolin (617.17 µg/g). In silico studies showed that bioactive compounds interfered with asthma and diarrhea-associated target genes, which are members of calcium-mediated signaling to exert a calcium channel blocker activity. The seeds extract exerted a concentration-dependent spasmolytic response on isolated jejunum, trachea, and urinary bladder preparations and caused relaxation of spastic contraction of K<sup>+</sup> (80 mM) with suppressed calcium concentration-response curves at dose 0.3 and 1 mg/mL. It also showed antiperistalsis, antidiarrheal and antisecretory activity in animal models. Thus, <i>C. sativus</i> seeds have therapeutic effects by regulating the contractile response through a calcium-mediated signaling pathway.
format	article
author	Muqeet Wahid Fatima Saqib Hanadi Talal Ahmedah Claudia Mihaela Gavris Vincenzo De Feo Mircea Hogea Marius Moga Radu Chicea
author_facet	Muqeet Wahid Fatima Saqib Hanadi Talal Ahmedah Claudia Mihaela Gavris Vincenzo De Feo Mircea Hogea Marius Moga Radu Chicea
author_sort	Muqeet Wahid
title	<i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway
title_short	<i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway
title_full	<i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway
title_fullStr	<i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway
title_full_unstemmed	<i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway
title_sort	<i>cucumis sativus</i> l. seeds ameliorate muscular spasm-induced gastrointestinal and respiratory disorders by simultaneously inhibiting calcium mediated signaling pathway
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3fd0f28cb90d4402a06f80387ca6c1ab
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<i>Cucumis sativus</i> L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway

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