Key protein-coding genes related to microglia in immune regulation and inflammatory response induced by epilepsy

Several studies have shown a link between immunity, inflammatory processes, and epilepsy. Active neuroinflammation and marked immune cell infiltration occur in epilepsy of diverse etiologies. Microglia, as the first line of defense in the central nervous system, are the main effectors of neuroinflam...

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Autores principales:	Jing Cao, Hui Gan, Han Xiao, Hui Chen, Dan Jian, Ning Jiang, Xuan Zhai
Formato:	article
Lenguaje:	EN
Publicado:	AIMS Press 2021
Materias:	immunity inflammatory processes epilepsy microglia differentially expressed genes protein-protein interaction Biotechnology TP248.13-248.65 Mathematics QA1-939
Acceso en línea:	https://doaj.org/article/3fd1b4aeb25c448f980d7fc0f90cb2dd
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spelling	oai:doaj.org-article:3fd1b4aeb25c448f980d7fc0f90cb2dd2021-11-29T06:24:51ZKey protein-coding genes related to microglia in immune regulation and inflammatory response induced by epilepsy10.3934/mbe.20214691551-0018https://doaj.org/article/3fd1b4aeb25c448f980d7fc0f90cb2dd2021-11-01T00:00:00Zhttps://www.aimspress.com/article/doi/10.3934/mbe.2021469?viewType=HTMLhttps://doaj.org/toc/1551-0018Several studies have shown a link between immunity, inflammatory processes, and epilepsy. Active neuroinflammation and marked immune cell infiltration occur in epilepsy of diverse etiologies. Microglia, as the first line of defense in the central nervous system, are the main effectors of neuroinflammatory processes. Discovery of new biomarkers associated with microglia activation after epileptogenesis indicates that targeting specific molecules may help control seizures. In this research, we used a combination of several bioinformatics approaches, including RNA sequencing, to explore differentially expressed genes (DEGs) in epileptic lesions and control samples, and to construct a protein-protein interaction (PPI) network for DEGs, which was examined utilizing plug-ins in Cytoscape software. Finally, we aimed to identify 10 hub genes in immune and inflammation-related sub-networks, which were subsequently validated in real-time quantitative polymerase chain reaction analysis in a mouse model of kainic acid-induced epilepsy. The expression patterns of nine genes were consistent with sequencing outcomes. Meanwhile, several genes, including CX3CR1, CX3CL1, GPR183, FPR1, P2RY13, P2RY12 and LPAR5, were associated with microglial activation and migration, providing novel candidate targets for immunotherapy in epilepsy and laying the foundation for further research.Jing Cao Hui GanHan XiaoHui ChenDan JianNing JiangXuan ZhaiAIMS Pressarticleimmunityinflammatory processesepilepsymicrogliadifferentially expressed genesprotein-protein interactionBiotechnologyTP248.13-248.65MathematicsQA1-939ENMathematical Biosciences and Engineering, Vol 18, Iss 6, Pp 9563-9578 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	immunity inflammatory processes epilepsy microglia differentially expressed genes protein-protein interaction Biotechnology TP248.13-248.65 Mathematics QA1-939
spellingShingle	immunity inflammatory processes epilepsy microglia differentially expressed genes protein-protein interaction Biotechnology TP248.13-248.65 Mathematics QA1-939 Jing Cao Hui Gan Han Xiao Hui Chen Dan Jian Ning Jiang Xuan Zhai Key protein-coding genes related to microglia in immune regulation and inflammatory response induced by epilepsy
description	Several studies have shown a link between immunity, inflammatory processes, and epilepsy. Active neuroinflammation and marked immune cell infiltration occur in epilepsy of diverse etiologies. Microglia, as the first line of defense in the central nervous system, are the main effectors of neuroinflammatory processes. Discovery of new biomarkers associated with microglia activation after epileptogenesis indicates that targeting specific molecules may help control seizures. In this research, we used a combination of several bioinformatics approaches, including RNA sequencing, to explore differentially expressed genes (DEGs) in epileptic lesions and control samples, and to construct a protein-protein interaction (PPI) network for DEGs, which was examined utilizing plug-ins in Cytoscape software. Finally, we aimed to identify 10 hub genes in immune and inflammation-related sub-networks, which were subsequently validated in real-time quantitative polymerase chain reaction analysis in a mouse model of kainic acid-induced epilepsy. The expression patterns of nine genes were consistent with sequencing outcomes. Meanwhile, several genes, including CX3CR1, CX3CL1, GPR183, FPR1, P2RY13, P2RY12 and LPAR5, were associated with microglial activation and migration, providing novel candidate targets for immunotherapy in epilepsy and laying the foundation for further research.
format	article
author	Jing Cao Hui Gan Han Xiao Hui Chen Dan Jian Ning Jiang Xuan Zhai
author_facet	Jing Cao Hui Gan Han Xiao Hui Chen Dan Jian Ning Jiang Xuan Zhai
author_sort	Jing Cao
title	Key protein-coding genes related to microglia in immune regulation and inflammatory response induced by epilepsy
title_short	Key protein-coding genes related to microglia in immune regulation and inflammatory response induced by epilepsy
title_full	Key protein-coding genes related to microglia in immune regulation and inflammatory response induced by epilepsy
title_fullStr	Key protein-coding genes related to microglia in immune regulation and inflammatory response induced by epilepsy
title_full_unstemmed	Key protein-coding genes related to microglia in immune regulation and inflammatory response induced by epilepsy
title_sort	key protein-coding genes related to microglia in immune regulation and inflammatory response induced by epilepsy
publisher	AIMS Press
publishDate	2021
url	https://doaj.org/article/3fd1b4aeb25c448f980d7fc0f90cb2dd
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_version_	1718407519781519360

Key protein-coding genes related to microglia in immune regulation and inflammatory response induced by epilepsy

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