Vibrio cholerae hemolysin is required for lethality, developmental delay, and intestinal vacuolation in Caenorhabditis elegans.

<h4>Background</h4>Cholera toxin (CT) and toxin-co-regulated pili (TCP) are the major virulence factors of Vibrio cholerae O1 and O139 strains that contribute to the pathogenesis of disease during devastating cholera pandemics. However, CT and TCP negative V. cholerae strains are still a...

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Autores principales:	Hediye Nese Cinar, Mahendra Kothary, Atin R Datta, Ben D Tall, Robert Sprando, Kivanc Bilecen, Fitnat Yildiz, Barbara McCardell
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3fdf9aacf4eb49718511ace5e23ed805
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spelling	oai:doaj.org-article:3fdf9aacf4eb49718511ace5e23ed8052021-12-02T20:20:08ZVibrio cholerae hemolysin is required for lethality, developmental delay, and intestinal vacuolation in Caenorhabditis elegans.1932-620310.1371/journal.pone.0011558https://doaj.org/article/3fdf9aacf4eb49718511ace5e23ed8052010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20644623/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Cholera toxin (CT) and toxin-co-regulated pili (TCP) are the major virulence factors of Vibrio cholerae O1 and O139 strains that contribute to the pathogenesis of disease during devastating cholera pandemics. However, CT and TCP negative V. cholerae strains are still able to cause severe diarrheal disease in humans through mechanisms that are not well understood.<h4>Methodology/principal findings</h4>To determine the role of other virulence factors in V. cholerae pathogenesis, we used a CT and TCP independent infection model in the nematode Caenorhabditis elegans and identified the hemolysin A (hlyA) gene as a factor responsible for animal death and developmental delay. We demonstrated a correlation between the severity of infection in the nematode and the level of hemolytic activity in the V. cholerae biotypes. At the cellular level, V. cholerae infection induces formation of vacuoles in the intestinal cells in a hlyA dependent manner, consistent with the previous in vitro observations.<h4>Conclusions/significance</h4>Our data strongly suggest that HlyA is a virulence factor in C. elegans infection leading to lethality and developmental delay presumably through intestinal cytopathic changes.Hediye Nese CinarMahendra KotharyAtin R DattaBen D TallRobert SprandoKivanc BilecenFitnat YildizBarbara McCardellPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 7, p e11558 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hediye Nese Cinar Mahendra Kothary Atin R Datta Ben D Tall Robert Sprando Kivanc Bilecen Fitnat Yildiz Barbara McCardell Vibrio cholerae hemolysin is required for lethality, developmental delay, and intestinal vacuolation in Caenorhabditis elegans.
description	<h4>Background</h4>Cholera toxin (CT) and toxin-co-regulated pili (TCP) are the major virulence factors of Vibrio cholerae O1 and O139 strains that contribute to the pathogenesis of disease during devastating cholera pandemics. However, CT and TCP negative V. cholerae strains are still able to cause severe diarrheal disease in humans through mechanisms that are not well understood.<h4>Methodology/principal findings</h4>To determine the role of other virulence factors in V. cholerae pathogenesis, we used a CT and TCP independent infection model in the nematode Caenorhabditis elegans and identified the hemolysin A (hlyA) gene as a factor responsible for animal death and developmental delay. We demonstrated a correlation between the severity of infection in the nematode and the level of hemolytic activity in the V. cholerae biotypes. At the cellular level, V. cholerae infection induces formation of vacuoles in the intestinal cells in a hlyA dependent manner, consistent with the previous in vitro observations.<h4>Conclusions/significance</h4>Our data strongly suggest that HlyA is a virulence factor in C. elegans infection leading to lethality and developmental delay presumably through intestinal cytopathic changes.
format	article
author	Hediye Nese Cinar Mahendra Kothary Atin R Datta Ben D Tall Robert Sprando Kivanc Bilecen Fitnat Yildiz Barbara McCardell
author_facet	Hediye Nese Cinar Mahendra Kothary Atin R Datta Ben D Tall Robert Sprando Kivanc Bilecen Fitnat Yildiz Barbara McCardell
author_sort	Hediye Nese Cinar
title	Vibrio cholerae hemolysin is required for lethality, developmental delay, and intestinal vacuolation in Caenorhabditis elegans.
title_short	Vibrio cholerae hemolysin is required for lethality, developmental delay, and intestinal vacuolation in Caenorhabditis elegans.
title_full	Vibrio cholerae hemolysin is required for lethality, developmental delay, and intestinal vacuolation in Caenorhabditis elegans.
title_fullStr	Vibrio cholerae hemolysin is required for lethality, developmental delay, and intestinal vacuolation in Caenorhabditis elegans.
title_full_unstemmed	Vibrio cholerae hemolysin is required for lethality, developmental delay, and intestinal vacuolation in Caenorhabditis elegans.
title_sort	vibrio cholerae hemolysin is required for lethality, developmental delay, and intestinal vacuolation in caenorhabditis elegans.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/3fdf9aacf4eb49718511ace5e23ed805
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Vibrio cholerae hemolysin is required for lethality, developmental delay, and intestinal vacuolation in Caenorhabditis elegans.

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