Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy

Abstract We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma. FLIM can be used to monitor or detect changes in the fluorescence lifetime of metabolic cofactor...

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Autores principales:	Taylor A. Hinsdale, Bilal H. Malik, Shuna Cheng, Oscar R. Benavides, Maryellen L. Giger, John M. Wright, Paras B. Patel, Javier A. Jo, Kristen C. Maitland
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3fe4e94ea55946189c029db792ed761b
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spelling	oai:doaj.org-article:3fe4e94ea55946189c029db792ed761b2021-12-02T13:30:12ZEnhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy10.1038/s41598-021-84552-82045-2322https://doaj.org/article/3fe4e94ea55946189c029db792ed761b2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84552-8https://doaj.org/toc/2045-2322Abstract We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma. FLIM can be used to monitor or detect changes in the fluorescence lifetime of metabolic cofactors (e.g. NADH and FAD) associated with the onset of carcinogenesis. However, out of focus fluorescence often interferes with this lifetime measurement. Structured illumination fluorescence lifetime imaging (SI-FLIM) addresses this by providing depth-resolved lifetime measurements, and applied to oral mucosa, can localize the collected signal to the epithelium. In this study, the hamster model of oral carcinogenesis was used to evaluate SI-FLIM in premalignant and malignant oral mucosa. Cheek pouches were imaged in vivo and correlated to histopathological diagnoses. The potential of NADH fluorescence signal and lifetime, as measured by widefield FLIM and SI-FLIM, to differentiate dysplasia (pre-malignancy) from normal tissue was evaluated. ROC analysis was carried out with the task of discriminating between normal tissue and mild dysplasia, when changes in fluorescence characteristics are localized to the epithelium only. The results demonstrate that SI-FLIM (AUC = 0.83) is a significantly better (p-value = 0.031) marker for mild dysplasia when compared to widefield FLIM (AUC = 0.63).Taylor A. HinsdaleBilal H. MalikShuna ChengOscar R. BenavidesMaryellen L. GigerJohn M. WrightParas B. PatelJavier A. JoKristen C. MaitlandNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Taylor A. Hinsdale Bilal H. Malik Shuna Cheng Oscar R. Benavides Maryellen L. Giger John M. Wright Paras B. Patel Javier A. Jo Kristen C. Maitland Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
description	Abstract We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma. FLIM can be used to monitor or detect changes in the fluorescence lifetime of metabolic cofactors (e.g. NADH and FAD) associated with the onset of carcinogenesis. However, out of focus fluorescence often interferes with this lifetime measurement. Structured illumination fluorescence lifetime imaging (SI-FLIM) addresses this by providing depth-resolved lifetime measurements, and applied to oral mucosa, can localize the collected signal to the epithelium. In this study, the hamster model of oral carcinogenesis was used to evaluate SI-FLIM in premalignant and malignant oral mucosa. Cheek pouches were imaged in vivo and correlated to histopathological diagnoses. The potential of NADH fluorescence signal and lifetime, as measured by widefield FLIM and SI-FLIM, to differentiate dysplasia (pre-malignancy) from normal tissue was evaluated. ROC analysis was carried out with the task of discriminating between normal tissue and mild dysplasia, when changes in fluorescence characteristics are localized to the epithelium only. The results demonstrate that SI-FLIM (AUC = 0.83) is a significantly better (p-value = 0.031) marker for mild dysplasia when compared to widefield FLIM (AUC = 0.63).
format	article
author	Taylor A. Hinsdale Bilal H. Malik Shuna Cheng Oscar R. Benavides Maryellen L. Giger John M. Wright Paras B. Patel Javier A. Jo Kristen C. Maitland
author_facet	Taylor A. Hinsdale Bilal H. Malik Shuna Cheng Oscar R. Benavides Maryellen L. Giger John M. Wright Paras B. Patel Javier A. Jo Kristen C. Maitland
author_sort	Taylor A. Hinsdale
title	Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
title_short	Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
title_full	Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
title_fullStr	Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
title_full_unstemmed	Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
title_sort	enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3fe4e94ea55946189c029db792ed761b
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Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy

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