Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression

Yanmei Xu, Yan Xiong, Chen Xu, Chuanwen Xu Department of Nephrology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan City, Hubei Province 430033, People’s Republic of ChinaCorrespondence: Chuanwen XuDepartment of Nephrology...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xu Y, Xiong Y, Xu C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	sp renal injury apoptosis mirna-145-5p tlr4/myd88/nf-κb(p65) Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/3fe54fa60c064bd392d6f416d858675d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3fe54fa60c064bd392d6f416d858675d
record_format	dspace
spelling	oai:doaj.org-article:3fe54fa60c064bd392d6f416d858675d2021-12-02T12:45:48ZStandard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression1178-7007https://doaj.org/article/3fe54fa60c064bd392d6f416d858675d2020-10-01T00:00:00Zhttps://www.dovepress.com/standard-puerarin-prevents-diabetic-renal-damage-by-inhibiting-mirna-1-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Yanmei Xu, Yan Xiong, Chen Xu, Chuanwen Xu Department of Nephrology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan City, Hubei Province 430033, People’s Republic of ChinaCorrespondence: Chuanwen XuDepartment of Nephrology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 473 Hanzheng Street, Qiaokou District, Wuhan City, Hubei Province 430033, People’s Republic of ChinaTel +86-18607171819Email xuchuanwen0425@tom.comAim: This study was designed to use in vivo and in vitro approaches to evaluate puerarin in diabetes-induced renal injury.Materials and Methods: SD rats were divided into NC (normal control), Model (diabetic induced renal injury model), SP-L (model rats treated with low-dose standard puerarin), SP-M (model rats treated with middle-dose standard puerarin), and SP-H (model rats treated with high-dose standard puerarin) groups. We evaluated fasting blood-glucose (FBG), urinary albumin/creatinine ratio (UACR), body weight, and kidney index (KI) in the different groups. TNF-α, IL-1β, and IL-6 concentrations were measured using Elisa assays. HE staining and TUNEL assays were used to evaluate pathology and apoptosis in kidney tissues, respectively. Relative gene and protein expression was measured using RT-qPCR and Western blot assays. Apoptosis was measured using flow cytometry. The correlation between miRNA-145-5p and TLR4 was assessed using dual-luciferase reporter gene assays.Results: The pathology and apoptosis cell number were deteriorate in Model group; TNF-α, IL-1β and IL-6 concentrations, FGB, UACR and KI were increased and body weight was depressed; meanwhile, relative gene and proteins expressions (miRNA-145-5p, TLR4, MyD88 and NF-κB p65) were significantly different in Model group in vivo and vitro study compared with NC group. SP treatment significantly improved the pathology and apoptosis levels in the tissues, as well as TNF-α, IL-1β and IL-6 concentrations, FGB, UACR, body weight, and KI. In vitro cell studies revealed that SP could prevent renal injury induced by diabetes through the miRNA-145-5p/TLR4 axis.Conclusion: SP prevents diabetes-induced renal damage via miRNA-145-5p overexpression and reduces TLR4/MyD88/NF-κB (p65) pathway activation in vitro and in vivo.Keywords: SP, renal injury, apoptosis, miRNA-145-5p, TLR4/MyD88/NF-κB (p65)Xu YXiong YXu CXu CDove Medical Pressarticlesprenal injuryapoptosismirna-145-5ptlr4/myd88/nf-κb(p65)Specialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 3947-3958 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	sp renal injury apoptosis mirna-145-5p tlr4/myd88/nf-κb(p65) Specialties of internal medicine RC581-951
spellingShingle	sp renal injury apoptosis mirna-145-5p tlr4/myd88/nf-κb(p65) Specialties of internal medicine RC581-951 Xu Y Xiong Y Xu C Xu C Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression
description	Yanmei Xu, Yan Xiong, Chen Xu, Chuanwen Xu Department of Nephrology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan City, Hubei Province 430033, People’s Republic of ChinaCorrespondence: Chuanwen XuDepartment of Nephrology, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 473 Hanzheng Street, Qiaokou District, Wuhan City, Hubei Province 430033, People’s Republic of ChinaTel +86-18607171819Email xuchuanwen0425@tom.comAim: This study was designed to use in vivo and in vitro approaches to evaluate puerarin in diabetes-induced renal injury.Materials and Methods: SD rats were divided into NC (normal control), Model (diabetic induced renal injury model), SP-L (model rats treated with low-dose standard puerarin), SP-M (model rats treated with middle-dose standard puerarin), and SP-H (model rats treated with high-dose standard puerarin) groups. We evaluated fasting blood-glucose (FBG), urinary albumin/creatinine ratio (UACR), body weight, and kidney index (KI) in the different groups. TNF-α, IL-1β, and IL-6 concentrations were measured using Elisa assays. HE staining and TUNEL assays were used to evaluate pathology and apoptosis in kidney tissues, respectively. Relative gene and protein expression was measured using RT-qPCR and Western blot assays. Apoptosis was measured using flow cytometry. The correlation between miRNA-145-5p and TLR4 was assessed using dual-luciferase reporter gene assays.Results: The pathology and apoptosis cell number were deteriorate in Model group; TNF-α, IL-1β and IL-6 concentrations, FGB, UACR and KI were increased and body weight was depressed; meanwhile, relative gene and proteins expressions (miRNA-145-5p, TLR4, MyD88 and NF-κB p65) were significantly different in Model group in vivo and vitro study compared with NC group. SP treatment significantly improved the pathology and apoptosis levels in the tissues, as well as TNF-α, IL-1β and IL-6 concentrations, FGB, UACR, body weight, and KI. In vitro cell studies revealed that SP could prevent renal injury induced by diabetes through the miRNA-145-5p/TLR4 axis.Conclusion: SP prevents diabetes-induced renal damage via miRNA-145-5p overexpression and reduces TLR4/MyD88/NF-κB (p65) pathway activation in vitro and in vivo.Keywords: SP, renal injury, apoptosis, miRNA-145-5p, TLR4/MyD88/NF-κB (p65)
format	article
author	Xu Y Xiong Y Xu C Xu C
author_facet	Xu Y Xiong Y Xu C Xu C
author_sort	Xu Y
title	Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression
title_short	Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression
title_full	Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression
title_fullStr	Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression
title_full_unstemmed	Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression
title_sort	standard puerarin prevents diabetic renal damage by inhibiting mirna-140-5p expression
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/3fe54fa60c064bd392d6f416d858675d
work_keys_str_mv	AT xuy standardpuerarinpreventsdiabeticrenaldamagebyinhibitingmirna1405pexpression AT xiongy standardpuerarinpreventsdiabeticrenaldamagebyinhibitingmirna1405pexpression AT xuc standardpuerarinpreventsdiabeticrenaldamagebyinhibitingmirna1405pexpression AT xuc standardpuerarinpreventsdiabeticrenaldamagebyinhibitingmirna1405pexpression
_version_	1718393680294838272

Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression

Ejemplares similares