Potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review.

Immunological tests may represent valuable tools for the diagnosis of human tegumentary leishmaniasis (TL) due to their simple execution, less invasive nature and potential use as a point-of-care test. Indeed, several antigenic targets have been used with the aim of improving the restricted scenario...

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Autores principales:	Mariana Lourenço Freire, Felipe Dutra Rêgo, Gláucia Cota, Marcelo Antônio Pascoal-Xavier, Edward Oliveira
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3fe648e3b24a43cb8179b4e39ec2a6dd
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spelling	oai:doaj.org-article:3fe648e3b24a43cb8179b4e39ec2a6dd2021-11-25T06:23:43ZPotential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review.1932-620310.1371/journal.pone.0251956https://doaj.org/article/3fe648e3b24a43cb8179b4e39ec2a6dd2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251956https://doaj.org/toc/1932-6203Immunological tests may represent valuable tools for the diagnosis of human tegumentary leishmaniasis (TL) due to their simple execution, less invasive nature and potential use as a point-of-care test. Indeed, several antigenic targets have been used with the aim of improving the restricted scenario for TL-diagnosis. We performed a worldwide systematic review to identify antigenic targets that have been evaluated for the main clinical forms of TL, such as cutaneous (CL) and mucosal (ML) leishmaniasis. Included were original studies evaluating the sensitivity and specificity of immunological tests for human-TL, CL and/or ML diagnosis using purified or recombinant proteins, synthetic peptides or polyclonal or monoclonal antibodies to detect Leishmania-specific antibodies or antigens. The review methodology followed PRISMA guidelines and all selected studies were evaluated in accordance with QUADAS-2. Thirty-eight original studies from four databases fulfilled the selection criteria. A total of 79 antigens were evaluated for the detection of antibodies as a diagnostic for TL, CL and/or ML by ELISA. Furthermore, three antibodies were evaluated for the detection of antigen by immunochromatographic test (ICT) and immunohistochemistry (IHC) for CL-diagnosis. Several antigenic targets showed 100% of sensitivity and specificity, suggesting potential use for TL-diagnosis in its different clinical manifestations. However, a high number of proof-of-concept studies reinforce the need for further analysis aimed at verifying true diagnostic accuracy in clinical practice.Mariana Lourenço FreireFelipe Dutra RêgoGláucia CotaMarcelo Antônio Pascoal-XavierEdward OliveiraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251956 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariana Lourenço Freire Felipe Dutra Rêgo Gláucia Cota Marcelo Antônio Pascoal-Xavier Edward Oliveira Potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review.
description	Immunological tests may represent valuable tools for the diagnosis of human tegumentary leishmaniasis (TL) due to their simple execution, less invasive nature and potential use as a point-of-care test. Indeed, several antigenic targets have been used with the aim of improving the restricted scenario for TL-diagnosis. We performed a worldwide systematic review to identify antigenic targets that have been evaluated for the main clinical forms of TL, such as cutaneous (CL) and mucosal (ML) leishmaniasis. Included were original studies evaluating the sensitivity and specificity of immunological tests for human-TL, CL and/or ML diagnosis using purified or recombinant proteins, synthetic peptides or polyclonal or monoclonal antibodies to detect Leishmania-specific antibodies or antigens. The review methodology followed PRISMA guidelines and all selected studies were evaluated in accordance with QUADAS-2. Thirty-eight original studies from four databases fulfilled the selection criteria. A total of 79 antigens were evaluated for the detection of antibodies as a diagnostic for TL, CL and/or ML by ELISA. Furthermore, three antibodies were evaluated for the detection of antigen by immunochromatographic test (ICT) and immunohistochemistry (IHC) for CL-diagnosis. Several antigenic targets showed 100% of sensitivity and specificity, suggesting potential use for TL-diagnosis in its different clinical manifestations. However, a high number of proof-of-concept studies reinforce the need for further analysis aimed at verifying true diagnostic accuracy in clinical practice.
format	article
author	Mariana Lourenço Freire Felipe Dutra Rêgo Gláucia Cota Marcelo Antônio Pascoal-Xavier Edward Oliveira
author_facet	Mariana Lourenço Freire Felipe Dutra Rêgo Gláucia Cota Marcelo Antônio Pascoal-Xavier Edward Oliveira
author_sort	Mariana Lourenço Freire
title	Potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review.
title_short	Potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review.
title_full	Potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review.
title_fullStr	Potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review.
title_full_unstemmed	Potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review.
title_sort	potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: a systematic review.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3fe648e3b24a43cb8179b4e39ec2a6dd
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Potential antigenic targets used in immunological tests for diagnosis of tegumentary leishmaniasis: A systematic review.

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