Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions

Abstract Cellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of s...

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Autores principales:	Ryota Sakamoto, Vincent Noireaux, Yusuke T. Maeda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3fed9043a938420d8689c93dd87d5455
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spelling	oai:doaj.org-article:3fed9043a938420d8689c93dd87d54552021-12-02T11:41:02ZAnomalous Scaling of Gene Expression in Confined Cell-Free Reactions10.1038/s41598-018-25532-32045-2322https://doaj.org/article/3fed9043a938420d8689c93dd87d54552018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25532-3https://doaj.org/toc/2045-2322Abstract Cellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of synthesized reporter proteins in droplets of radius R shows an anomalous geometric scaling of R 4 different from the expected size-dependence of R 3. Given that TXTL becomes less efficient at thin surface layer, a mathematical model explains the anomalous size-dependence found in experiment. The surface of cell-sized compartment can thus play a regulatory role for cell-free gene expression.Ryota SakamotoVincent NoireauxYusuke T. MaedaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryota Sakamoto Vincent Noireaux Yusuke T. Maeda Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions
description	Abstract Cellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of synthesized reporter proteins in droplets of radius R shows an anomalous geometric scaling of R 4 different from the expected size-dependence of R 3. Given that TXTL becomes less efficient at thin surface layer, a mathematical model explains the anomalous size-dependence found in experiment. The surface of cell-sized compartment can thus play a regulatory role for cell-free gene expression.
format	article
author	Ryota Sakamoto Vincent Noireaux Yusuke T. Maeda
author_facet	Ryota Sakamoto Vincent Noireaux Yusuke T. Maeda
author_sort	Ryota Sakamoto
title	Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions
title_short	Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions
title_full	Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions
title_fullStr	Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions
title_full_unstemmed	Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions
title_sort	anomalous scaling of gene expression in confined cell-free reactions
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/3fed9043a938420d8689c93dd87d5455
work_keys_str_mv	AT ryotasakamoto anomalousscalingofgeneexpressioninconfinedcellfreereactions AT vincentnoireaux anomalousscalingofgeneexpressioninconfinedcellfreereactions AT yusuketmaeda anomalousscalingofgeneexpressioninconfinedcellfreereactions
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Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions

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