Emergent synchronous beating behavior in spontaneous beating cardiomyocyte clusters

Abstract We investigated the dominant rule determining synchronization of beating intervals of cardiomyocytes after the clustering of mouse primary and human embryonic-stem-cell (hES)-derived cardiomyocytes. Cardiomyocyte clusters were formed in concave agarose cultivation chambers and their beating...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kazufumi Sakamoto, Yoshitsune Hondo, Naoki Takahashi, Yuhei Tanaka, Rikuto Sekine, Kenji Shimoda, Haruki Watanabe, Kenji Yasuda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3fef93f6dedf4055a424b67f1a96be84
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3fef93f6dedf4055a424b67f1a96be84
record_format	dspace
spelling	oai:doaj.org-article:3fef93f6dedf4055a424b67f1a96be842021-12-02T17:51:06ZEmergent synchronous beating behavior in spontaneous beating cardiomyocyte clusters10.1038/s41598-021-91466-y2045-2322https://doaj.org/article/3fef93f6dedf4055a424b67f1a96be842021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91466-yhttps://doaj.org/toc/2045-2322Abstract We investigated the dominant rule determining synchronization of beating intervals of cardiomyocytes after the clustering of mouse primary and human embryonic-stem-cell (hES)-derived cardiomyocytes. Cardiomyocyte clusters were formed in concave agarose cultivation chambers and their beating intervals were compared with those of dispersed isolated single cells. Distribution analysis revealed that the clusters’ synchronized interbeat intervals (IBIs) were longer than the majority of those of isolated single cells, which is against the conventional faster firing regulation or “overdrive suppression.” IBI distribution of the isolated individual cardiomyocytes acquired from the beating clusters also confirmed that the clusters’ IBI was longer than those of the majority of constituent cardiomyocytes. In the complementary experiment in which cell clusters were connected together and then separated again, two cardiomyocyte clusters having different IBIs were attached and synchronized to the longer IBIs than those of the two clusters’ original IBIs, and recovered to shorter IBIs after their separation. This is not only against overdrive suppression but also mathematical synchronization models, such as the Kuramoto model, in which synchronized beating becomes intermediate between the two clusters’ IBIs. These results suggest that emergent slower synchronous beating occurred in homogeneous cardiomyocyte clusters as a community effect of spontaneously beating cells.Kazufumi SakamotoYoshitsune HondoNaoki TakahashiYuhei TanakaRikuto SekineKenji ShimodaHaruki WatanabeKenji YasudaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kazufumi Sakamoto Yoshitsune Hondo Naoki Takahashi Yuhei Tanaka Rikuto Sekine Kenji Shimoda Haruki Watanabe Kenji Yasuda Emergent synchronous beating behavior in spontaneous beating cardiomyocyte clusters
description	Abstract We investigated the dominant rule determining synchronization of beating intervals of cardiomyocytes after the clustering of mouse primary and human embryonic-stem-cell (hES)-derived cardiomyocytes. Cardiomyocyte clusters were formed in concave agarose cultivation chambers and their beating intervals were compared with those of dispersed isolated single cells. Distribution analysis revealed that the clusters’ synchronized interbeat intervals (IBIs) were longer than the majority of those of isolated single cells, which is against the conventional faster firing regulation or “overdrive suppression.” IBI distribution of the isolated individual cardiomyocytes acquired from the beating clusters also confirmed that the clusters’ IBI was longer than those of the majority of constituent cardiomyocytes. In the complementary experiment in which cell clusters were connected together and then separated again, two cardiomyocyte clusters having different IBIs were attached and synchronized to the longer IBIs than those of the two clusters’ original IBIs, and recovered to shorter IBIs after their separation. This is not only against overdrive suppression but also mathematical synchronization models, such as the Kuramoto model, in which synchronized beating becomes intermediate between the two clusters’ IBIs. These results suggest that emergent slower synchronous beating occurred in homogeneous cardiomyocyte clusters as a community effect of spontaneously beating cells.
format	article
author	Kazufumi Sakamoto Yoshitsune Hondo Naoki Takahashi Yuhei Tanaka Rikuto Sekine Kenji Shimoda Haruki Watanabe Kenji Yasuda
author_facet	Kazufumi Sakamoto Yoshitsune Hondo Naoki Takahashi Yuhei Tanaka Rikuto Sekine Kenji Shimoda Haruki Watanabe Kenji Yasuda
author_sort	Kazufumi Sakamoto
title	Emergent synchronous beating behavior in spontaneous beating cardiomyocyte clusters
title_short	Emergent synchronous beating behavior in spontaneous beating cardiomyocyte clusters
title_full	Emergent synchronous beating behavior in spontaneous beating cardiomyocyte clusters
title_fullStr	Emergent synchronous beating behavior in spontaneous beating cardiomyocyte clusters
title_full_unstemmed	Emergent synchronous beating behavior in spontaneous beating cardiomyocyte clusters
title_sort	emergent synchronous beating behavior in spontaneous beating cardiomyocyte clusters
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3fef93f6dedf4055a424b67f1a96be84
work_keys_str_mv	AT kazufumisakamoto emergentsynchronousbeatingbehaviorinspontaneousbeatingcardiomyocyteclusters AT yoshitsunehondo emergentsynchronousbeatingbehaviorinspontaneousbeatingcardiomyocyteclusters AT naokitakahashi emergentsynchronousbeatingbehaviorinspontaneousbeatingcardiomyocyteclusters AT yuheitanaka emergentsynchronousbeatingbehaviorinspontaneousbeatingcardiomyocyteclusters AT rikutosekine emergentsynchronousbeatingbehaviorinspontaneousbeatingcardiomyocyteclusters AT kenjishimoda emergentsynchronousbeatingbehaviorinspontaneousbeatingcardiomyocyteclusters AT harukiwatanabe emergentsynchronousbeatingbehaviorinspontaneousbeatingcardiomyocyteclusters AT kenjiyasuda emergentsynchronousbeatingbehaviorinspontaneousbeatingcardiomyocyteclusters
_version_	1718379280715481088

Emergent synchronous beating behavior in spontaneous beating cardiomyocyte clusters

Ejemplares similares