An Inquiry-Based Laboratory Module to Promote Understanding of the Scientific Method and Bacterial Conjugation

Students are engaged and improve their critical thinking skills in laboratory courses when they have the opportunity to design and conduct inquiry-based experiments that generate novel results. A discovery-driven project for a microbiology, genetics, or multidisciplinary research laboratory course w...

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Autores principales:	Melanie B. Berkmen, Anastasia C. Murthy, Matthew P. Broulidakis
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3fefbab3477740b59f5fd2f1b9c4e469
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spelling	oai:doaj.org-article:3fefbab3477740b59f5fd2f1b9c4e4692021-11-15T15:15:37ZAn Inquiry-Based Laboratory Module to Promote Understanding of the Scientific Method and Bacterial Conjugation10.1128/jmbe.v15i2.7631935-78851935-7877https://doaj.org/article/3fefbab3477740b59f5fd2f1b9c4e4692014-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v15i2.763https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Students are engaged and improve their critical thinking skills in laboratory courses when they have the opportunity to design and conduct inquiry-based experiments that generate novel results. A discovery-driven project for a microbiology, genetics, or multidisciplinary research laboratory course was developed to familiarize students with the scientific method. In this multi-lab module, students determine whether their chosen stress conditions induce conjugation and/or cell death of the model BSL-1 Gram-positive bacterium Bacillus subtilis. Through consultation of the primary literature, students identify conditions or chemicals that can elicit DNA damage, the SOS response, and/or cellular stress. In groups, students discuss their selected conditions, develop their hypotheses and experimental plans, and formulate their positive and negative controls. Students then subject the B. subtilis donor cells to the stress conditions, mix donors with recipients to allow mating, and plate serial dilutions of the mixtures on selective plates to measure how the treatments affect conjugation frequency and donor cell viability. Finally, students analyze and discuss their collective data in light of their controls. The goals of this module are to encourage students to be actively involved in the scientific process while contributing to our understanding of the conditions that stimulate horizontal gene transfer in bacteria.Melanie B. BerkmenAnastasia C. MurthyMatthew P. BroulidakisAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 15, Iss 2, Pp 321-322 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Melanie B. Berkmen Anastasia C. Murthy Matthew P. Broulidakis An Inquiry-Based Laboratory Module to Promote Understanding of the Scientific Method and Bacterial Conjugation
description	Students are engaged and improve their critical thinking skills in laboratory courses when they have the opportunity to design and conduct inquiry-based experiments that generate novel results. A discovery-driven project for a microbiology, genetics, or multidisciplinary research laboratory course was developed to familiarize students with the scientific method. In this multi-lab module, students determine whether their chosen stress conditions induce conjugation and/or cell death of the model BSL-1 Gram-positive bacterium Bacillus subtilis. Through consultation of the primary literature, students identify conditions or chemicals that can elicit DNA damage, the SOS response, and/or cellular stress. In groups, students discuss their selected conditions, develop their hypotheses and experimental plans, and formulate their positive and negative controls. Students then subject the B. subtilis donor cells to the stress conditions, mix donors with recipients to allow mating, and plate serial dilutions of the mixtures on selective plates to measure how the treatments affect conjugation frequency and donor cell viability. Finally, students analyze and discuss their collective data in light of their controls. The goals of this module are to encourage students to be actively involved in the scientific process while contributing to our understanding of the conditions that stimulate horizontal gene transfer in bacteria.
format	article
author	Melanie B. Berkmen Anastasia C. Murthy Matthew P. Broulidakis
author_facet	Melanie B. Berkmen Anastasia C. Murthy Matthew P. Broulidakis
author_sort	Melanie B. Berkmen
title	An Inquiry-Based Laboratory Module to Promote Understanding of the Scientific Method and Bacterial Conjugation
title_short	An Inquiry-Based Laboratory Module to Promote Understanding of the Scientific Method and Bacterial Conjugation
title_full	An Inquiry-Based Laboratory Module to Promote Understanding of the Scientific Method and Bacterial Conjugation
title_fullStr	An Inquiry-Based Laboratory Module to Promote Understanding of the Scientific Method and Bacterial Conjugation
title_full_unstemmed	An Inquiry-Based Laboratory Module to Promote Understanding of the Scientific Method and Bacterial Conjugation
title_sort	inquiry-based laboratory module to promote understanding of the scientific method and bacterial conjugation
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/3fefbab3477740b59f5fd2f1b9c4e469
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An Inquiry-Based Laboratory Module to Promote Understanding of the Scientific Method and Bacterial Conjugation

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