Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture

Improved culture methods are needed to reliably grow human pluripotent stem cells (hPSCs) on a large scale. Here, the authors identify a xeno-free medium with a supplement of Inter-α-inhibitor that supports long-term propagation and improved single-cell passaging of hPSCs on uncoated plastic.

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Autores principales:	Sara Pijuan-Galitó, Christoffer Tamm, Jens Schuster, Maria Sobol, Lars Forsberg, Catherine L. R. Merry, Cecilia Annerén
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/3ff4aa12a81242048dcf3aaa0077653e
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spelling	oai:doaj.org-article:3ff4aa12a81242048dcf3aaa0077653e2021-12-02T15:35:23ZHuman serum-derived protein removes the need for coating in defined human pluripotent stem cell culture10.1038/ncomms121702041-1723https://doaj.org/article/3ff4aa12a81242048dcf3aaa0077653e2016-07-01T00:00:00Zhttps://doi.org/10.1038/ncomms12170https://doaj.org/toc/2041-1723Improved culture methods are needed to reliably grow human pluripotent stem cells (hPSCs) on a large scale. Here, the authors identify a xeno-free medium with a supplement of Inter-α-inhibitor that supports long-term propagation and improved single-cell passaging of hPSCs on uncoated plastic.Sara Pijuan-GalitóChristoffer TammJens SchusterMaria SobolLars ForsbergCatherine L. R. MerryCecilia AnnerénNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-12 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Sara Pijuan-Galitó Christoffer Tamm Jens Schuster Maria Sobol Lars Forsberg Catherine L. R. Merry Cecilia Annerén Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture
description	Improved culture methods are needed to reliably grow human pluripotent stem cells (hPSCs) on a large scale. Here, the authors identify a xeno-free medium with a supplement of Inter-α-inhibitor that supports long-term propagation and improved single-cell passaging of hPSCs on uncoated plastic.
format	article
author	Sara Pijuan-Galitó Christoffer Tamm Jens Schuster Maria Sobol Lars Forsberg Catherine L. R. Merry Cecilia Annerén
author_facet	Sara Pijuan-Galitó Christoffer Tamm Jens Schuster Maria Sobol Lars Forsberg Catherine L. R. Merry Cecilia Annerén
author_sort	Sara Pijuan-Galitó
title	Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture
title_short	Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture
title_full	Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture
title_fullStr	Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture
title_full_unstemmed	Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture
title_sort	human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/3ff4aa12a81242048dcf3aaa0077653e
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Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture

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