Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture

Improved culture methods are needed to reliably grow human pluripotent stem cells (hPSCs) on a large scale. Here, the authors identify a xeno-free medium with a supplement of Inter-α-inhibitor that supports long-term propagation and improved single-cell passaging of hPSCs on uncoated plastic.

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Autores principales: Sara Pijuan-Galitó, Christoffer Tamm, Jens Schuster, Maria Sobol, Lars Forsberg, Catherine L. R. Merry, Cecilia Annerén
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/3ff4aa12a81242048dcf3aaa0077653e
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