Rac1 dynamics in the human opportunistic fungal pathogen Candida albicans.

The small Rho G-protein Rac1 is highly conserved from fungi to humans, with approximately 65% overall sequence identity in Candida albicans. As observed with human Rac1, we show that C. albicans Rac1 can accumulate in the nucleus, and fluorescence recovery after photobleaching (FRAP) together with f...

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Autores principales:	Romain Vauchelles, Danièle Stalder, Thomas Botton, Robert A Arkowitz, Martine Bassilana
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ff508bab04740159580a8b0ed207777
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spelling	oai:doaj.org-article:3ff508bab04740159580a8b0ed2077772021-11-18T07:02:43ZRac1 dynamics in the human opportunistic fungal pathogen Candida albicans.1932-620310.1371/journal.pone.0015400https://doaj.org/article/3ff508bab04740159580a8b0ed2077772010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21060846/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The small Rho G-protein Rac1 is highly conserved from fungi to humans, with approximately 65% overall sequence identity in Candida albicans. As observed with human Rac1, we show that C. albicans Rac1 can accumulate in the nucleus, and fluorescence recovery after photobleaching (FRAP) together with fluorescence loss in photobleaching (FLIP) studies indicate that this Rho G-protein undergoes nucleo-cytoplasmic shuttling. Analyses of different chimeras revealed that nuclear accumulation of C. albicans Rac1 requires the NLS-motifs at its carboxyl-terminus, which are blocked by prenylation of the adjacent cysteine residue. Furthermore, we show that C. albicans Rac1 dynamics, both at the plasma membrane and in the nucleus, are dependent on its activation state and in particular that the inactive form accumulates faster in the nucleus. Heterologous expression of human Rac1 in C. albicans also results in nuclear accumulation, yet accumulation is more rapid than that of C. albicans Rac1. Taken together our results indicate that Rac1 nuclear accumulation is an inherent property of this G-protein and suggest that the requirements for its nucleo-cytoplasmic shuttling are conserved from fungi to humans.Romain VauchellesDanièle StalderThomas BottonRobert A ArkowitzMartine BassilanaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10, p e15400 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Romain Vauchelles Danièle Stalder Thomas Botton Robert A Arkowitz Martine Bassilana Rac1 dynamics in the human opportunistic fungal pathogen Candida albicans.
description	The small Rho G-protein Rac1 is highly conserved from fungi to humans, with approximately 65% overall sequence identity in Candida albicans. As observed with human Rac1, we show that C. albicans Rac1 can accumulate in the nucleus, and fluorescence recovery after photobleaching (FRAP) together with fluorescence loss in photobleaching (FLIP) studies indicate that this Rho G-protein undergoes nucleo-cytoplasmic shuttling. Analyses of different chimeras revealed that nuclear accumulation of C. albicans Rac1 requires the NLS-motifs at its carboxyl-terminus, which are blocked by prenylation of the adjacent cysteine residue. Furthermore, we show that C. albicans Rac1 dynamics, both at the plasma membrane and in the nucleus, are dependent on its activation state and in particular that the inactive form accumulates faster in the nucleus. Heterologous expression of human Rac1 in C. albicans also results in nuclear accumulation, yet accumulation is more rapid than that of C. albicans Rac1. Taken together our results indicate that Rac1 nuclear accumulation is an inherent property of this G-protein and suggest that the requirements for its nucleo-cytoplasmic shuttling are conserved from fungi to humans.
format	article
author	Romain Vauchelles Danièle Stalder Thomas Botton Robert A Arkowitz Martine Bassilana
author_facet	Romain Vauchelles Danièle Stalder Thomas Botton Robert A Arkowitz Martine Bassilana
author_sort	Romain Vauchelles
title	Rac1 dynamics in the human opportunistic fungal pathogen Candida albicans.
title_short	Rac1 dynamics in the human opportunistic fungal pathogen Candida albicans.
title_full	Rac1 dynamics in the human opportunistic fungal pathogen Candida albicans.
title_fullStr	Rac1 dynamics in the human opportunistic fungal pathogen Candida albicans.
title_full_unstemmed	Rac1 dynamics in the human opportunistic fungal pathogen Candida albicans.
title_sort	rac1 dynamics in the human opportunistic fungal pathogen candida albicans.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/3ff508bab04740159580a8b0ed207777
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Rac1 dynamics in the human opportunistic fungal pathogen Candida albicans.

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