Profiling and identification of small rDNA-derived RNAs and their potential biological functions.

Small non-coding RNAs constitute a large family of regulatory molecules with diverse functions. Notably, some small non-coding RNAs matched to rDNA have been identified as qiRNAs and small guide RNAs involved in various biological processes. However, a large number of small rDNA-derived RNAs (srRNAs...

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Autores principales:	Haibin Wei, Ben Zhou, Fang Zhang, Yanyang Tu, Yanan Hu, Baoguo Zhang, Qiwei Zhai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ffaf0032ce64b1999eab07b0f0025fb
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spelling	oai:doaj.org-article:3ffaf0032ce64b1999eab07b0f0025fb2021-11-18T07:57:34ZProfiling and identification of small rDNA-derived RNAs and their potential biological functions.1932-620310.1371/journal.pone.0056842https://doaj.org/article/3ffaf0032ce64b1999eab07b0f0025fb2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23418607/?tool=EBIhttps://doaj.org/toc/1932-6203Small non-coding RNAs constitute a large family of regulatory molecules with diverse functions. Notably, some small non-coding RNAs matched to rDNA have been identified as qiRNAs and small guide RNAs involved in various biological processes. However, a large number of small rDNA-derived RNAs (srRNAs) are usually neglected and yet to be investigated. We systematically investigated srRNAs using small RNA datasets generated by high-throughput sequencing, and found srRNAs are mainly mapped to rRNA coding regions in sense direction. The datasets from immunoprecipitation and high-throughput sequencing demonstrate that srRNAs are co-immunoprecipitated with Argonaute (AGO) proteins. Furthermore, the srRNA expression profile in mouse liver is affected by diabetes. Overexpression or inhibition of srRNAs in cultured cells shows that srRNAs are involved in various signaling pathways. This study presents a global view of srRNAs in total small RNA and AGO protein complex from different species, and demonstrates that srRNAs are correlated with diabetes, and involved in some biological processes. These findings provide new insights into srRNAs and their functions in various physiological and pathological processes.Haibin WeiBen ZhouFang ZhangYanyang TuYanan HuBaoguo ZhangQiwei ZhaiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e56842 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haibin Wei Ben Zhou Fang Zhang Yanyang Tu Yanan Hu Baoguo Zhang Qiwei Zhai Profiling and identification of small rDNA-derived RNAs and their potential biological functions.
description	Small non-coding RNAs constitute a large family of regulatory molecules with diverse functions. Notably, some small non-coding RNAs matched to rDNA have been identified as qiRNAs and small guide RNAs involved in various biological processes. However, a large number of small rDNA-derived RNAs (srRNAs) are usually neglected and yet to be investigated. We systematically investigated srRNAs using small RNA datasets generated by high-throughput sequencing, and found srRNAs are mainly mapped to rRNA coding regions in sense direction. The datasets from immunoprecipitation and high-throughput sequencing demonstrate that srRNAs are co-immunoprecipitated with Argonaute (AGO) proteins. Furthermore, the srRNA expression profile in mouse liver is affected by diabetes. Overexpression or inhibition of srRNAs in cultured cells shows that srRNAs are involved in various signaling pathways. This study presents a global view of srRNAs in total small RNA and AGO protein complex from different species, and demonstrates that srRNAs are correlated with diabetes, and involved in some biological processes. These findings provide new insights into srRNAs and their functions in various physiological and pathological processes.
format	article
author	Haibin Wei Ben Zhou Fang Zhang Yanyang Tu Yanan Hu Baoguo Zhang Qiwei Zhai
author_facet	Haibin Wei Ben Zhou Fang Zhang Yanyang Tu Yanan Hu Baoguo Zhang Qiwei Zhai
author_sort	Haibin Wei
title	Profiling and identification of small rDNA-derived RNAs and their potential biological functions.
title_short	Profiling and identification of small rDNA-derived RNAs and their potential biological functions.
title_full	Profiling and identification of small rDNA-derived RNAs and their potential biological functions.
title_fullStr	Profiling and identification of small rDNA-derived RNAs and their potential biological functions.
title_full_unstemmed	Profiling and identification of small rDNA-derived RNAs and their potential biological functions.
title_sort	profiling and identification of small rdna-derived rnas and their potential biological functions.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3ffaf0032ce64b1999eab07b0f0025fb
work_keys_str_mv	AT haibinwei profilingandidentificationofsmallrdnaderivedrnasandtheirpotentialbiologicalfunctions AT benzhou profilingandidentificationofsmallrdnaderivedrnasandtheirpotentialbiologicalfunctions AT fangzhang profilingandidentificationofsmallrdnaderivedrnasandtheirpotentialbiologicalfunctions AT yanyangtu profilingandidentificationofsmallrdnaderivedrnasandtheirpotentialbiologicalfunctions AT yananhu profilingandidentificationofsmallrdnaderivedrnasandtheirpotentialbiologicalfunctions AT baoguozhang profilingandidentificationofsmallrdnaderivedrnasandtheirpotentialbiologicalfunctions AT qiweizhai profilingandidentificationofsmallrdnaderivedrnasandtheirpotentialbiologicalfunctions
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Profiling and identification of small rDNA-derived RNAs and their potential biological functions.

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