Epimorphin regulates bile duct formation via effects on mitosis orientation in rat liver epithelial stem-like cells.

Understanding how hepatic precursor cells can generate differentiated bile ducts is crucial for studies on epithelial morphogenesis and for development of cell therapies for hepatobiliary diseases. Epimorphin (EPM) is a key morphogen for duct morphogenesis in various epithelial organs. The role of E...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Junnian Zhou, Lei Zhao, Lipeng Qin, Jing Wang, Yali Jia, Hailei Yao, Chen Sang, Qinghua Hu, Shuangshuang Shi, Xue Nan, Wen Yue, Fengyuan Zhuang, Chun Yang, Yunfang Wang, Xuetao Pei
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2010
Materias:
R
Q
Acceso en línea:https://doaj.org/article/4000d8e41f30450a8221bf1b33477dd2
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:4000d8e41f30450a8221bf1b33477dd2
record_format dspace
spelling oai:doaj.org-article:4000d8e41f30450a8221bf1b33477dd22021-11-25T06:25:23ZEpimorphin regulates bile duct formation via effects on mitosis orientation in rat liver epithelial stem-like cells.1932-620310.1371/journal.pone.0009732https://doaj.org/article/4000d8e41f30450a8221bf1b33477dd22010-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20305811/?tool=EBIhttps://doaj.org/toc/1932-6203Understanding how hepatic precursor cells can generate differentiated bile ducts is crucial for studies on epithelial morphogenesis and for development of cell therapies for hepatobiliary diseases. Epimorphin (EPM) is a key morphogen for duct morphogenesis in various epithelial organs. The role of EPM in bile duct formation (DF) from hepatic precursor cells, however, is not known. To address this issue, we used WB-F344 rat epithelial stem-like cells as model for bile duct formation. A micropattern and a uniaxial static stretch device was used to investigate the effects of EPM and stress fiber bundles on the mitosis orientation (MO) of WB cells. Immunohistochemistry of liver tissue sections demonstrated high EPM expression around bile ducts in vivo. In vitro, recombinant EPM selectively induced DF through upregulation of CK19 expression and suppression of HNF3alpha and HNF6, with no effects on other hepatocytic genes investigated. Our data provide evidence that EPM guides MO of WB-F344 cells via effects on stress fiber bundles and focal adhesion assembly, as supported by blockade EPM, beta1 integrin, and F-actin assembly. These blockers can also inhibit EPM-induced DF. These results demonstrate a new biophysical action of EPM in bile duct formation, during which determination of MO plays a crucial role.Junnian ZhouLei ZhaoLipeng QinJing WangYali JiaHailei YaoChen SangQinghua HuShuangshuang ShiXue NanWen YueFengyuan ZhuangChun YangYunfang WangXuetao PeiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 3, p e9732 (2010)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Junnian Zhou
Lei Zhao
Lipeng Qin
Jing Wang
Yali Jia
Hailei Yao
Chen Sang
Qinghua Hu
Shuangshuang Shi
Xue Nan
Wen Yue
Fengyuan Zhuang
Chun Yang
Yunfang Wang
Xuetao Pei
Epimorphin regulates bile duct formation via effects on mitosis orientation in rat liver epithelial stem-like cells.
description Understanding how hepatic precursor cells can generate differentiated bile ducts is crucial for studies on epithelial morphogenesis and for development of cell therapies for hepatobiliary diseases. Epimorphin (EPM) is a key morphogen for duct morphogenesis in various epithelial organs. The role of EPM in bile duct formation (DF) from hepatic precursor cells, however, is not known. To address this issue, we used WB-F344 rat epithelial stem-like cells as model for bile duct formation. A micropattern and a uniaxial static stretch device was used to investigate the effects of EPM and stress fiber bundles on the mitosis orientation (MO) of WB cells. Immunohistochemistry of liver tissue sections demonstrated high EPM expression around bile ducts in vivo. In vitro, recombinant EPM selectively induced DF through upregulation of CK19 expression and suppression of HNF3alpha and HNF6, with no effects on other hepatocytic genes investigated. Our data provide evidence that EPM guides MO of WB-F344 cells via effects on stress fiber bundles and focal adhesion assembly, as supported by blockade EPM, beta1 integrin, and F-actin assembly. These blockers can also inhibit EPM-induced DF. These results demonstrate a new biophysical action of EPM in bile duct formation, during which determination of MO plays a crucial role.
format article
author Junnian Zhou
Lei Zhao
Lipeng Qin
Jing Wang
Yali Jia
Hailei Yao
Chen Sang
Qinghua Hu
Shuangshuang Shi
Xue Nan
Wen Yue
Fengyuan Zhuang
Chun Yang
Yunfang Wang
Xuetao Pei
author_facet Junnian Zhou
Lei Zhao
Lipeng Qin
Jing Wang
Yali Jia
Hailei Yao
Chen Sang
Qinghua Hu
Shuangshuang Shi
Xue Nan
Wen Yue
Fengyuan Zhuang
Chun Yang
Yunfang Wang
Xuetao Pei
author_sort Junnian Zhou
title Epimorphin regulates bile duct formation via effects on mitosis orientation in rat liver epithelial stem-like cells.
title_short Epimorphin regulates bile duct formation via effects on mitosis orientation in rat liver epithelial stem-like cells.
title_full Epimorphin regulates bile duct formation via effects on mitosis orientation in rat liver epithelial stem-like cells.
title_fullStr Epimorphin regulates bile duct formation via effects on mitosis orientation in rat liver epithelial stem-like cells.
title_full_unstemmed Epimorphin regulates bile duct formation via effects on mitosis orientation in rat liver epithelial stem-like cells.
title_sort epimorphin regulates bile duct formation via effects on mitosis orientation in rat liver epithelial stem-like cells.
publisher Public Library of Science (PLoS)
publishDate 2010
url https://doaj.org/article/4000d8e41f30450a8221bf1b33477dd2
work_keys_str_mv AT junnianzhou epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
AT leizhao epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
AT lipengqin epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
AT jingwang epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
AT yalijia epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
AT haileiyao epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
AT chensang epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
AT qinghuahu epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
AT shuangshuangshi epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
AT xuenan epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
AT wenyue epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
AT fengyuanzhuang epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
AT chunyang epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
AT yunfangwang epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
AT xuetaopei epimorphinregulatesbileductformationviaeffectsonmitosisorientationinratliverepithelialstemlikecells
_version_ 1718413737521577984