In vitro evaluation of simulated stereotactic radiotherapy for wet age-related macular degeneration on three different cell lines

Abstract Low energy stereotactic radiotherapy has been proposed for the treatment of neovascular age related macular degeneration. We investigated the in vitro effect of the radiotherapy on pericytes, retinal pigment epithelium and endothelial cells. Primary human retinal pigment epithelium cells, h...

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Autores principales:	Efstathios Vounotrypidis, Anna Hillenmayer, Christian M. Wertheimer, Alexis Athanasiou, Jakob Siedlecki, Michael Orth, Andreas Ohlmann, Siegfried G. Priglinger, Armin Wolf
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/40035aca30294a2d9f55526ce30804c4
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spelling	oai:doaj.org-article:40035aca30294a2d9f55526ce30804c42021-12-02T14:28:00ZIn vitro evaluation of simulated stereotactic radiotherapy for wet age-related macular degeneration on three different cell lines10.1038/s41598-021-87466-72045-2322https://doaj.org/article/40035aca30294a2d9f55526ce30804c42021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87466-7https://doaj.org/toc/2045-2322Abstract Low energy stereotactic radiotherapy has been proposed for the treatment of neovascular age related macular degeneration. We investigated the in vitro effect of the radiotherapy on pericytes, retinal pigment epithelium and endothelial cells. Primary human retinal pigment epithelium cells, human umbilical vein endothelial cells and human pericytes from Placenta were cultivated. In a pairwise protocol, one plate was irradiated at a dose of 16 Gy, while the second plate served as a non-irradiated control. Thereafter, cells were cultivated either in serum-free (non-permissive) or serum-stimulated (permissive) conditions. A life/dead assay, an XTT and a BrdU assay were performed up to 7 days after irradiation. No cell death occurred at any timepoint in any cell line after treatment nor in the control. Compared to the unirradiated controls, cell viability and metabolic activity were significantly reduced in irradiated cells in the XTT assay, except for non-permissive RPE cells. In the BrdU assay, proliferation was inhibited. While no cell death was detected in vitro, viability and proliferative capacity of all cell lines were significantly reduced. Therefore, it seems that low energy stereotactic radiotherapy inhibits angiogenesis without a direct induction of apoptosis but influencing microvascular function and stability.Efstathios VounotrypidisAnna HillenmayerChristian M. WertheimerAlexis AthanasiouJakob SiedleckiMichael OrthAndreas OhlmannSiegfried G. PriglingerArmin WolfNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Efstathios Vounotrypidis Anna Hillenmayer Christian M. Wertheimer Alexis Athanasiou Jakob Siedlecki Michael Orth Andreas Ohlmann Siegfried G. Priglinger Armin Wolf In vitro evaluation of simulated stereotactic radiotherapy for wet age-related macular degeneration on three different cell lines
description	Abstract Low energy stereotactic radiotherapy has been proposed for the treatment of neovascular age related macular degeneration. We investigated the in vitro effect of the radiotherapy on pericytes, retinal pigment epithelium and endothelial cells. Primary human retinal pigment epithelium cells, human umbilical vein endothelial cells and human pericytes from Placenta were cultivated. In a pairwise protocol, one plate was irradiated at a dose of 16 Gy, while the second plate served as a non-irradiated control. Thereafter, cells were cultivated either in serum-free (non-permissive) or serum-stimulated (permissive) conditions. A life/dead assay, an XTT and a BrdU assay were performed up to 7 days after irradiation. No cell death occurred at any timepoint in any cell line after treatment nor in the control. Compared to the unirradiated controls, cell viability and metabolic activity were significantly reduced in irradiated cells in the XTT assay, except for non-permissive RPE cells. In the BrdU assay, proliferation was inhibited. While no cell death was detected in vitro, viability and proliferative capacity of all cell lines were significantly reduced. Therefore, it seems that low energy stereotactic radiotherapy inhibits angiogenesis without a direct induction of apoptosis but influencing microvascular function and stability.
format	article
author	Efstathios Vounotrypidis Anna Hillenmayer Christian M. Wertheimer Alexis Athanasiou Jakob Siedlecki Michael Orth Andreas Ohlmann Siegfried G. Priglinger Armin Wolf
author_facet	Efstathios Vounotrypidis Anna Hillenmayer Christian M. Wertheimer Alexis Athanasiou Jakob Siedlecki Michael Orth Andreas Ohlmann Siegfried G. Priglinger Armin Wolf
author_sort	Efstathios Vounotrypidis
title	In vitro evaluation of simulated stereotactic radiotherapy for wet age-related macular degeneration on three different cell lines
title_short	In vitro evaluation of simulated stereotactic radiotherapy for wet age-related macular degeneration on three different cell lines
title_full	In vitro evaluation of simulated stereotactic radiotherapy for wet age-related macular degeneration on three different cell lines
title_fullStr	In vitro evaluation of simulated stereotactic radiotherapy for wet age-related macular degeneration on three different cell lines
title_full_unstemmed	In vitro evaluation of simulated stereotactic radiotherapy for wet age-related macular degeneration on three different cell lines
title_sort	in vitro evaluation of simulated stereotactic radiotherapy for wet age-related macular degeneration on three different cell lines
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/40035aca30294a2d9f55526ce30804c4
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In vitro evaluation of simulated stereotactic radiotherapy for wet age-related macular degeneration on three different cell lines

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