Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China

Abstract Enterovirus A90 (EV-A90) is a novel serotype of enterovirus A species that is rarely reported. Here, we isolated five enteroviruses from patients with acute flaccid paralysis in Hotan and Kashgar cities in Xinjiang, China that were identified as EV-A90 by molecular typing. The VP1 sequences...

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Autores principales:	Keqiang Huang, Yong Zhang, Yang Song, Hui Cui, Dongmei Yan, Shuangli Zhu, Qiang Sun, Haishu Tang, Dongyan Wang, Wenbo Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/400ad1ffe31b4fd9a03b36ba36b0c3f2
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spelling	oai:doaj.org-article:400ad1ffe31b4fd9a03b36ba36b0c3f22021-12-02T15:09:07ZAntigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China10.1038/s41598-018-28469-92045-2322https://doaj.org/article/400ad1ffe31b4fd9a03b36ba36b0c3f22018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-28469-9https://doaj.org/toc/2045-2322Abstract Enterovirus A90 (EV-A90) is a novel serotype of enterovirus A species that is rarely reported. Here, we isolated five enteroviruses from patients with acute flaccid paralysis in Hotan and Kashgar cities in Xinjiang, China that were identified as EV-A90 by molecular typing. The VP1 sequences of these Xinjiang EV-A90 strains showed 88.4–89% nucleotide sequence identity to the prototype EV-A90 strain; however, genome analysis indicated complex recombination events in P2 and P3 regions. Next, the seroprevalence of EV-A90 was examined in 49 serum specimens collected in Hotan and Kashgar, and 37.5% were EV-A90 antibody positive (>1:8), with a geometric mean titre (GMT) of 1:10.47. The low positive rate and GMT suggest a low-level EV-A90 epidemic in Xinjiang. Two of the five Xinjiang EV-A90 strains were temperature sensitive, and three were temperature resistant, and a comparative genomics analysis suggested that an amino acid substitution (H1799Y) in the 3Dpol region was related to temperature sensitivity. Although the epidemic strength is low, some EV-A90 strains were temperature resistant, which is suggestive of strong virulence and transmission capacity. This study expanded the number of EV-A90 in GenBank and provided basic data that may be useful for studying the molecular epidemiology of EV-A90.Keqiang HuangYong ZhangYang SongHui CuiDongmei YanShuangli ZhuQiang SunHaishu TangDongyan WangWenbo XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keqiang Huang Yong Zhang Yang Song Hui Cui Dongmei Yan Shuangli Zhu Qiang Sun Haishu Tang Dongyan Wang Wenbo Xu Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China
description	Abstract Enterovirus A90 (EV-A90) is a novel serotype of enterovirus A species that is rarely reported. Here, we isolated five enteroviruses from patients with acute flaccid paralysis in Hotan and Kashgar cities in Xinjiang, China that were identified as EV-A90 by molecular typing. The VP1 sequences of these Xinjiang EV-A90 strains showed 88.4–89% nucleotide sequence identity to the prototype EV-A90 strain; however, genome analysis indicated complex recombination events in P2 and P3 regions. Next, the seroprevalence of EV-A90 was examined in 49 serum specimens collected in Hotan and Kashgar, and 37.5% were EV-A90 antibody positive (>1:8), with a geometric mean titre (GMT) of 1:10.47. The low positive rate and GMT suggest a low-level EV-A90 epidemic in Xinjiang. Two of the five Xinjiang EV-A90 strains were temperature sensitive, and three were temperature resistant, and a comparative genomics analysis suggested that an amino acid substitution (H1799Y) in the 3Dpol region was related to temperature sensitivity. Although the epidemic strength is low, some EV-A90 strains were temperature resistant, which is suggestive of strong virulence and transmission capacity. This study expanded the number of EV-A90 in GenBank and provided basic data that may be useful for studying the molecular epidemiology of EV-A90.
format	article
author	Keqiang Huang Yong Zhang Yang Song Hui Cui Dongmei Yan Shuangli Zhu Qiang Sun Haishu Tang Dongyan Wang Wenbo Xu
author_facet	Keqiang Huang Yong Zhang Yang Song Hui Cui Dongmei Yan Shuangli Zhu Qiang Sun Haishu Tang Dongyan Wang Wenbo Xu
author_sort	Keqiang Huang
title	Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China
title_short	Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China
title_full	Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China
title_fullStr	Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China
title_full_unstemmed	Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China
title_sort	antigenic characteristics and genomic analysis of novel ev-a90 enteroviruses isolated in xinjiang, china
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/400ad1ffe31b4fd9a03b36ba36b0c3f2
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Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China

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