Parvovirus b19 DNA CpG dinucleotide methylation and epigenetic regulation of viral expression.

CpG DNA methylation is one of the main epigenetic modifications playing a role in the control of gene expression. For DNA viruses whose genome has the ability to integrate in the host genome or to maintain as a latent episome, a correlation has been found between the extent of DNA methylation and vi...

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Autores principales:	Francesca Bonvicini, Elisabetta Manaresi, Francesca Di Furio, Luisa De Falco, Giorgio Gallinella
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/400b2b78e0984caf8597fe3e6b9dd47b
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spelling	oai:doaj.org-article:400b2b78e0984caf8597fe3e6b9dd47b2021-11-18T07:25:47ZParvovirus b19 DNA CpG dinucleotide methylation and epigenetic regulation of viral expression.1932-620310.1371/journal.pone.0033316https://doaj.org/article/400b2b78e0984caf8597fe3e6b9dd47b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22413013/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203CpG DNA methylation is one of the main epigenetic modifications playing a role in the control of gene expression. For DNA viruses whose genome has the ability to integrate in the host genome or to maintain as a latent episome, a correlation has been found between the extent of DNA methylation and viral quiescence. No information is available for Parvovirus B19, a human pathogenic virus, which is capable of both lytic and persistent infections. Within Parvovirus B19 genome, the inverted terminal regions display all the characteristic signatures of a genomic CpG island; therefore we hypothesised a role of CpG dinucleotide methylation in the regulation of viral genome expression.The analysis of CpG dinucleotide methylation of Parvovirus B19 DNA was carried out by an aptly designed quantitative real-time PCR assay on bisulfite-modified DNA. The effects of CpG methylation on the regulation of viral genome expression were first investigated by transfection of either unmethylated or in vitro methylated viral DNA in a model cell line, showing that methylation of viral DNA was correlated to lower expression levels of the viral genome. Then, in the course of in vitro infections in different cellular environments, it was observed that absence of viral expression and genome replication were both correlated to increasing levels of CpG methylation of viral DNA. Finally, the presence of CpG methylation was documented in viral DNA present in bioptic samples, indicating the occurrence and a possible role of this epigenetic modification in the course of natural infections.The presence of an epigenetic level of regulation of viral genome expression, possibly correlated to the silencing of the viral genome and contributing to the maintenance of the virus in tissues, can be relevant to the balance and outcome of the different types of infection associated to Parvovirus B19.Francesca BonviciniElisabetta ManaresiFrancesca Di FurioLuisa De FalcoGiorgio GallinellaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e33316 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francesca Bonvicini Elisabetta Manaresi Francesca Di Furio Luisa De Falco Giorgio Gallinella Parvovirus b19 DNA CpG dinucleotide methylation and epigenetic regulation of viral expression.
description	CpG DNA methylation is one of the main epigenetic modifications playing a role in the control of gene expression. For DNA viruses whose genome has the ability to integrate in the host genome or to maintain as a latent episome, a correlation has been found between the extent of DNA methylation and viral quiescence. No information is available for Parvovirus B19, a human pathogenic virus, which is capable of both lytic and persistent infections. Within Parvovirus B19 genome, the inverted terminal regions display all the characteristic signatures of a genomic CpG island; therefore we hypothesised a role of CpG dinucleotide methylation in the regulation of viral genome expression.The analysis of CpG dinucleotide methylation of Parvovirus B19 DNA was carried out by an aptly designed quantitative real-time PCR assay on bisulfite-modified DNA. The effects of CpG methylation on the regulation of viral genome expression were first investigated by transfection of either unmethylated or in vitro methylated viral DNA in a model cell line, showing that methylation of viral DNA was correlated to lower expression levels of the viral genome. Then, in the course of in vitro infections in different cellular environments, it was observed that absence of viral expression and genome replication were both correlated to increasing levels of CpG methylation of viral DNA. Finally, the presence of CpG methylation was documented in viral DNA present in bioptic samples, indicating the occurrence and a possible role of this epigenetic modification in the course of natural infections.The presence of an epigenetic level of regulation of viral genome expression, possibly correlated to the silencing of the viral genome and contributing to the maintenance of the virus in tissues, can be relevant to the balance and outcome of the different types of infection associated to Parvovirus B19.
format	article
author	Francesca Bonvicini Elisabetta Manaresi Francesca Di Furio Luisa De Falco Giorgio Gallinella
author_facet	Francesca Bonvicini Elisabetta Manaresi Francesca Di Furio Luisa De Falco Giorgio Gallinella
author_sort	Francesca Bonvicini
title	Parvovirus b19 DNA CpG dinucleotide methylation and epigenetic regulation of viral expression.
title_short	Parvovirus b19 DNA CpG dinucleotide methylation and epigenetic regulation of viral expression.
title_full	Parvovirus b19 DNA CpG dinucleotide methylation and epigenetic regulation of viral expression.
title_fullStr	Parvovirus b19 DNA CpG dinucleotide methylation and epigenetic regulation of viral expression.
title_full_unstemmed	Parvovirus b19 DNA CpG dinucleotide methylation and epigenetic regulation of viral expression.
title_sort	parvovirus b19 dna cpg dinucleotide methylation and epigenetic regulation of viral expression.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/400b2b78e0984caf8597fe3e6b9dd47b
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Parvovirus b19 DNA CpG dinucleotide methylation and epigenetic regulation of viral expression.

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