Alginate oligosaccharides can maintain activities of lysosomes under low pH condition

Abstract The objective of this study was to report that lysosome extracted from egg white could be used as a drug through oral administration for treating diseases by using pH sensitive alginate oligosaccharides. Lysosome-alginate oligosaccharides composite were formulated for oral administration of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ra-Mi Park, Ngoc-Han Thi Nguyen, Su-Min Lee, Yang-Hoon Kim, Jiho Min
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4012f44b384546a3bce4f8cc532fe171
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4012f44b384546a3bce4f8cc532fe171
record_format	dspace
spelling	oai:doaj.org-article:4012f44b384546a3bce4f8cc532fe1712021-12-02T17:51:29ZAlginate oligosaccharides can maintain activities of lysosomes under low pH condition10.1038/s41598-021-91175-62045-2322https://doaj.org/article/4012f44b384546a3bce4f8cc532fe1712021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91175-6https://doaj.org/toc/2045-2322Abstract The objective of this study was to report that lysosome extracted from egg white could be used as a drug through oral administration for treating diseases by using pH sensitive alginate oligosaccharides. Lysosome-alginate oligosaccharides composite were formulated for oral administration of lysosomes. The dissolution test confirmed the availability of the oral dosage form. When lysosome were used as an independent drug, the activity of protein was lost due to influence of low pH. Its antibacterial activity was also remarkably reduced. However, when lysosome-alginate oligosaccharides composite form was used, antimicrobial activity of lysozyme was maintained. At low pH, a gel-like matrix was formed by alginate oligosaccharides to protect the lysosome. When the pH was increased, alginate oligosaccharides were dissolved and the lysosome was released. SDS–polyacrylamide gel electrophoresis analysis of released lysosomes revealed that alginate oligosaccharide could effectively protect the lysosome from degradation or hydrolysis under acidic conditions for at least 2 h. The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.Ra-Mi ParkNgoc-Han Thi NguyenSu-Min LeeYang-Hoon KimJiho MinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ra-Mi Park Ngoc-Han Thi Nguyen Su-Min Lee Yang-Hoon Kim Jiho Min Alginate oligosaccharides can maintain activities of lysosomes under low pH condition
description	Abstract The objective of this study was to report that lysosome extracted from egg white could be used as a drug through oral administration for treating diseases by using pH sensitive alginate oligosaccharides. Lysosome-alginate oligosaccharides composite were formulated for oral administration of lysosomes. The dissolution test confirmed the availability of the oral dosage form. When lysosome were used as an independent drug, the activity of protein was lost due to influence of low pH. Its antibacterial activity was also remarkably reduced. However, when lysosome-alginate oligosaccharides composite form was used, antimicrobial activity of lysozyme was maintained. At low pH, a gel-like matrix was formed by alginate oligosaccharides to protect the lysosome. When the pH was increased, alginate oligosaccharides were dissolved and the lysosome was released. SDS–polyacrylamide gel electrophoresis analysis of released lysosomes revealed that alginate oligosaccharide could effectively protect the lysosome from degradation or hydrolysis under acidic conditions for at least 2 h. The results of this study are important for application of lysosomes as therapeutic agents, and also it was confirmed that alginate oligosaccharides have potential as direct delivery system for the oral application of protein derived therapies.
format	article
author	Ra-Mi Park Ngoc-Han Thi Nguyen Su-Min Lee Yang-Hoon Kim Jiho Min
author_facet	Ra-Mi Park Ngoc-Han Thi Nguyen Su-Min Lee Yang-Hoon Kim Jiho Min
author_sort	Ra-Mi Park
title	Alginate oligosaccharides can maintain activities of lysosomes under low pH condition
title_short	Alginate oligosaccharides can maintain activities of lysosomes under low pH condition
title_full	Alginate oligosaccharides can maintain activities of lysosomes under low pH condition
title_fullStr	Alginate oligosaccharides can maintain activities of lysosomes under low pH condition
title_full_unstemmed	Alginate oligosaccharides can maintain activities of lysosomes under low pH condition
title_sort	alginate oligosaccharides can maintain activities of lysosomes under low ph condition
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4012f44b384546a3bce4f8cc532fe171
work_keys_str_mv	AT ramipark alginateoligosaccharidescanmaintainactivitiesoflysosomesunderlowphcondition AT ngochanthinguyen alginateoligosaccharidescanmaintainactivitiesoflysosomesunderlowphcondition AT suminlee alginateoligosaccharidescanmaintainactivitiesoflysosomesunderlowphcondition AT yanghoonkim alginateoligosaccharidescanmaintainactivitiesoflysosomesunderlowphcondition AT jihomin alginateoligosaccharidescanmaintainactivitiesoflysosomesunderlowphcondition
_version_	1718379221557968896

Alginate oligosaccharides can maintain activities of lysosomes under low pH condition

Ejemplares similares