microRNA Expression Profiles in Myocardium of High-Fat Diet-Induced Obesity Rat

Huimin Yang,* Xin Xin,* Hang Yu, Yandong Bao, Pengyu Jia, Nan Wu, Dalin Jia Department of Cardiology, The Central Laboratory, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, People’s Republic of China*These authors contributed equally to this workCorresponden...

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Autores principales:	Yang H, Xin X, Yu H, Bao Y, Jia P, Wu N, Jia D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	high fat diet obesity microrna cardiac dysfunction cardiac remodeling Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/40194700921b4c53b8ae3243a3e18a87
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spelling	oai:doaj.org-article:40194700921b4c53b8ae3243a3e18a872021-12-02T09:35:47ZmicroRNA Expression Profiles in Myocardium of High-Fat Diet-Induced Obesity Rat1178-7007https://doaj.org/article/40194700921b4c53b8ae3243a3e18a872020-04-01T00:00:00Zhttps://www.dovepress.com/microrna-expression-profiles-in-myocardium-of-high-fat-diet-induced-ob-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Huimin Yang,* Xin Xin,* Hang Yu, Yandong Bao, Pengyu Jia, Nan Wu, Dalin Jia Department of Cardiology, The Central Laboratory, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, People’s Republic of China*These authors contributed equally to this workCorrespondence: Dalin Jia; Nan WuDepartment of Cardiology The Central Laboratory, The First Affiliated Hospital of China Medical University, 155th North of Nanjing Street, Heping District, Shenyang, Liaoning 110001, People’s Republic of ChinaEmail jdl2001@126.com; imwunan@163.comPurpose: A high-fat diet (HFD) can lead to cardiac dysfunction, hypertrophy, and fibrosis. This study aimed to explore microRNA expression profiles in the myocardium of HFD-induced obesity rat.Materials and Methods: Wistar rats were randomly divided into two groups, and fed with normal chow diet (NCD) or HFD for 20 weeks. Cardiac function was evaluated by echocardiography. Left ventricular myocardium was harvested to assess the extent of myocardial morphology alteration. MicroRNA expression was analyzed using Agilent miRNA microarray and quantitative real-time PCR (qRT-PCR) was used to validate the microarray data. The mirdbV6 database was used to forecast the miRNA target genes. The role of microRNAs in palmitate-induced cardiac hypertrophy and fibrosis in primary neonatal rat cardiomyocytes was evaluated by loss- and gain-of-function experiments.Results: Significant changes in cardiac function, hypertrophy, fibrosis, and apoptosis were found in HFD rats as compared with NCD rats. miR-141-3p and miR-144-3p were also significantly upregulated in the myocardium of HFD-induced obesity rat. A series of genes involved in essential biological processes, including anatomical structure development and metabolic process, was targeted by these two miRNAs. These target genes were also implicated in signaling pathways involved in the PI3K-Akt signaling pathway, Wnt signaling pathway, autophagy, and protein processing in the endoplasmic reticulum. Inhibition of miR-141 or overexpression of miR-144 attenuated palmitate-induced cardiac hypertrophy and fibrosis. In contrast, overexpression of miR-141 or inhibition of miR-144 aggravated palmitate-induced cardiac hypertrophy and fibrosis.Conclusion: This study identifies that miR-141 and miR-144 are candidate miRNAs associated with the development of HFD-induced cardiac dysfunction and structure alteration.Keywords: high-fat diet, obesity, microRNA, cardiac dysfunction, cardiac remodelingYang HXin XYu HBao YJia PWu NJia DDove Medical Pressarticlehigh fat dietobesitymicrornacardiac dysfunctioncardiac remodelingSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 1147-1159 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	high fat diet obesity microrna cardiac dysfunction cardiac remodeling Specialties of internal medicine RC581-951
spellingShingle	high fat diet obesity microrna cardiac dysfunction cardiac remodeling Specialties of internal medicine RC581-951 Yang H Xin X Yu H Bao Y Jia P Wu N Jia D microRNA Expression Profiles in Myocardium of High-Fat Diet-Induced Obesity Rat
description	Huimin Yang,* Xin Xin,* Hang Yu, Yandong Bao, Pengyu Jia, Nan Wu, Dalin Jia Department of Cardiology, The Central Laboratory, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, People’s Republic of China*These authors contributed equally to this workCorrespondence: Dalin Jia; Nan WuDepartment of Cardiology The Central Laboratory, The First Affiliated Hospital of China Medical University, 155th North of Nanjing Street, Heping District, Shenyang, Liaoning 110001, People’s Republic of ChinaEmail jdl2001@126.com; imwunan@163.comPurpose: A high-fat diet (HFD) can lead to cardiac dysfunction, hypertrophy, and fibrosis. This study aimed to explore microRNA expression profiles in the myocardium of HFD-induced obesity rat.Materials and Methods: Wistar rats were randomly divided into two groups, and fed with normal chow diet (NCD) or HFD for 20 weeks. Cardiac function was evaluated by echocardiography. Left ventricular myocardium was harvested to assess the extent of myocardial morphology alteration. MicroRNA expression was analyzed using Agilent miRNA microarray and quantitative real-time PCR (qRT-PCR) was used to validate the microarray data. The mirdbV6 database was used to forecast the miRNA target genes. The role of microRNAs in palmitate-induced cardiac hypertrophy and fibrosis in primary neonatal rat cardiomyocytes was evaluated by loss- and gain-of-function experiments.Results: Significant changes in cardiac function, hypertrophy, fibrosis, and apoptosis were found in HFD rats as compared with NCD rats. miR-141-3p and miR-144-3p were also significantly upregulated in the myocardium of HFD-induced obesity rat. A series of genes involved in essential biological processes, including anatomical structure development and metabolic process, was targeted by these two miRNAs. These target genes were also implicated in signaling pathways involved in the PI3K-Akt signaling pathway, Wnt signaling pathway, autophagy, and protein processing in the endoplasmic reticulum. Inhibition of miR-141 or overexpression of miR-144 attenuated palmitate-induced cardiac hypertrophy and fibrosis. In contrast, overexpression of miR-141 or inhibition of miR-144 aggravated palmitate-induced cardiac hypertrophy and fibrosis.Conclusion: This study identifies that miR-141 and miR-144 are candidate miRNAs associated with the development of HFD-induced cardiac dysfunction and structure alteration.Keywords: high-fat diet, obesity, microRNA, cardiac dysfunction, cardiac remodeling
format	article
author	Yang H Xin X Yu H Bao Y Jia P Wu N Jia D
author_facet	Yang H Xin X Yu H Bao Y Jia P Wu N Jia D
author_sort	Yang H
title	microRNA Expression Profiles in Myocardium of High-Fat Diet-Induced Obesity Rat
title_short	microRNA Expression Profiles in Myocardium of High-Fat Diet-Induced Obesity Rat
title_full	microRNA Expression Profiles in Myocardium of High-Fat Diet-Induced Obesity Rat
title_fullStr	microRNA Expression Profiles in Myocardium of High-Fat Diet-Induced Obesity Rat
title_full_unstemmed	microRNA Expression Profiles in Myocardium of High-Fat Diet-Induced Obesity Rat
title_sort	microrna expression profiles in myocardium of high-fat diet-induced obesity rat
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/40194700921b4c53b8ae3243a3e18a87
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microRNA Expression Profiles in Myocardium of High-Fat Diet-Induced Obesity Rat

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