The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection.

<h4>Background</h4>Trypanosoma cruzi is the etiological agent of Chagas disease, a debilitating illness that affects millions of people in the Americas. A major finding of the T. cruzi genome project was the discovery of a novel multigene family composed of approximately 1,300 genes that...

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Autores principales: Sara Lopes dos Santos, Leandro Martins Freitas, Francisco Pereira Lobo, Gabriela Flávia Rodrigues-Luiz, Tiago Antônio de Oliveira Mendes, Anny Carolline Silva Oliveira, Luciana Oliveira Andrade, Egler Chiari, Ricardo Tostes Gazzinelli, Santuza Maria Ribeiro Teixeira, Ricardo Toshio Fujiwara, Daniella Castanheira Bartholomeu
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spelling oai:doaj.org-article:401f16ced1e04073b01e04e44a8cebc82021-11-18T09:13:55ZThe MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection.1935-27271935-273510.1371/journal.pntd.0001779https://doaj.org/article/401f16ced1e04073b01e04e44a8cebc82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22905275/pdf/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Trypanosoma cruzi is the etiological agent of Chagas disease, a debilitating illness that affects millions of people in the Americas. A major finding of the T. cruzi genome project was the discovery of a novel multigene family composed of approximately 1,300 genes that encode mucin-associated surface proteins (MASPs). The high level of polymorphism of the MASP family associated with its localization at the surface of infective forms of the parasite suggests that MASP participates in host-parasite interactions. We speculate that the large repertoire of MASP sequences may contribute to the ability of T. cruzi to infect several host cell types and/or participate in host immune evasion mechanisms.<h4>Methods</h4>By sequencing seven cDNA libraries, we analyzed the MASP expression profile in trypomastigotes derived from distinct host cells and after sequential passages in acutely infected mice. Additionally, to investigate the MASP antigenic profile, we performed B-cell epitope prediction on MASP proteins and designed a MASP-specific peptide array with 110 putative epitopes, which was screened with sera from acutely infected mice.<h4>Findings and conclusions</h4>We observed differential expression of a few MASP genes between trypomastigotes derived from epithelial and myoblast cell lines. The more pronounced MASP expression changes were observed between bloodstream and tissue-culture trypomastigotes and between bloodstream forms from sequential passages in acutely infected mice. Moreover, we demonstrated that different MASP members were expressed during the acute T. cruzi infection and constitute parasite antigens that are recognized by IgG and IgM antibodies. We also found that distinct MASP peptides could trigger different antibody responses and that the antibody level against a given peptide may vary after sequential passages in mice. We speculate that changes in the large repertoire of MASP antigenic peptides during an infection may contribute to the evasion of host immune responses during the acute phase of Chagas disease.Sara Lopes dos SantosLeandro Martins FreitasFrancisco Pereira LoboGabriela Flávia Rodrigues-LuizTiago Antônio de Oliveira MendesAnny Carolline Silva OliveiraLuciana Oliveira AndradeEgler ChiariRicardo Tostes GazzinelliSantuza Maria Ribeiro TeixeiraRicardo Toshio FujiwaraDaniella Castanheira BartholomeuPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 6, Iss 8, p e1779 (2012)
institution DOAJ
collection DOAJ
language EN
topic Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
spellingShingle Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
Sara Lopes dos Santos
Leandro Martins Freitas
Francisco Pereira Lobo
Gabriela Flávia Rodrigues-Luiz
Tiago Antônio de Oliveira Mendes
Anny Carolline Silva Oliveira
Luciana Oliveira Andrade
Egler Chiari
Ricardo Tostes Gazzinelli
Santuza Maria Ribeiro Teixeira
Ricardo Toshio Fujiwara
Daniella Castanheira Bartholomeu
The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection.
description <h4>Background</h4>Trypanosoma cruzi is the etiological agent of Chagas disease, a debilitating illness that affects millions of people in the Americas. A major finding of the T. cruzi genome project was the discovery of a novel multigene family composed of approximately 1,300 genes that encode mucin-associated surface proteins (MASPs). The high level of polymorphism of the MASP family associated with its localization at the surface of infective forms of the parasite suggests that MASP participates in host-parasite interactions. We speculate that the large repertoire of MASP sequences may contribute to the ability of T. cruzi to infect several host cell types and/or participate in host immune evasion mechanisms.<h4>Methods</h4>By sequencing seven cDNA libraries, we analyzed the MASP expression profile in trypomastigotes derived from distinct host cells and after sequential passages in acutely infected mice. Additionally, to investigate the MASP antigenic profile, we performed B-cell epitope prediction on MASP proteins and designed a MASP-specific peptide array with 110 putative epitopes, which was screened with sera from acutely infected mice.<h4>Findings and conclusions</h4>We observed differential expression of a few MASP genes between trypomastigotes derived from epithelial and myoblast cell lines. The more pronounced MASP expression changes were observed between bloodstream and tissue-culture trypomastigotes and between bloodstream forms from sequential passages in acutely infected mice. Moreover, we demonstrated that different MASP members were expressed during the acute T. cruzi infection and constitute parasite antigens that are recognized by IgG and IgM antibodies. We also found that distinct MASP peptides could trigger different antibody responses and that the antibody level against a given peptide may vary after sequential passages in mice. We speculate that changes in the large repertoire of MASP antigenic peptides during an infection may contribute to the evasion of host immune responses during the acute phase of Chagas disease.
format article
author Sara Lopes dos Santos
Leandro Martins Freitas
Francisco Pereira Lobo
Gabriela Flávia Rodrigues-Luiz
Tiago Antônio de Oliveira Mendes
Anny Carolline Silva Oliveira
Luciana Oliveira Andrade
Egler Chiari
Ricardo Tostes Gazzinelli
Santuza Maria Ribeiro Teixeira
Ricardo Toshio Fujiwara
Daniella Castanheira Bartholomeu
author_facet Sara Lopes dos Santos
Leandro Martins Freitas
Francisco Pereira Lobo
Gabriela Flávia Rodrigues-Luiz
Tiago Antônio de Oliveira Mendes
Anny Carolline Silva Oliveira
Luciana Oliveira Andrade
Egler Chiari
Ricardo Tostes Gazzinelli
Santuza Maria Ribeiro Teixeira
Ricardo Toshio Fujiwara
Daniella Castanheira Bartholomeu
author_sort Sara Lopes dos Santos
title The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection.
title_short The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection.
title_full The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection.
title_fullStr The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection.
title_full_unstemmed The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection.
title_sort masp family of trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/401f16ced1e04073b01e04e44a8cebc8
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