Toward an Improved Meningococcal Serogroup B Assay

ABSTRACT Because of diverse sequences and differential expression of surface structures on individual invasive Neisseria meningitidis serogroup B (MenB) strains, predicting the efficacy of MenB vaccines using traditional human serum bactericidal assays (hSBA) is impractical. The meningococcal antige...

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Autor principal:	Jan Poolman
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	meningococcus serogroup B Neisseria meningitidis factor H binding protein vaccines Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/40246ba6b9ad40deadc77a30f334797e
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spelling	oai:doaj.org-article:40246ba6b9ad40deadc77a30f334797e2021-11-15T16:00:27ZToward an Improved Meningococcal Serogroup B Assay10.1128/mBio.00713-182150-7511https://doaj.org/article/40246ba6b9ad40deadc77a30f334797e2018-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00713-18https://doaj.org/toc/2150-7511ABSTRACT Because of diverse sequences and differential expression of surface structures on individual invasive Neisseria meningitidis serogroup B (MenB) strains, predicting the efficacy of MenB vaccines using traditional human serum bactericidal assays (hSBA) is impractical. The meningococcal antigen surface expression (MEASURE) assay uses flow cytometry to quantitate the expression of factor H binding proteins (fHbp) contained in the bivalent rLP2086 MenB vaccine. To date, experience with MEASURE has been lacking, and in a long-awaited article, McNeil et al. (mBio 9:e00036-18, https://doi.org/10.1128/mBio.00036-18), provide detailed mapping of a cross-reactive antibody binding epitope and explore the potential utility of MEASURE in predicting the susceptibility of individual MenB strains to antibody-mediated killing. Remaining questions center around why some strains with high fHbp expression are nonsusceptible to anti-fHbp antibody killing. Consideration of alternative methods, such as a standardized enzyme-linked immunosorbent assay (ELISA), might offer a more readily available and reproducible assay for wider use.Jan PoolmanAmerican Society for Microbiologyarticlemeningococcus serogroup BNeisseria meningitidisfactor H binding proteinvaccinesMicrobiologyQR1-502ENmBio, Vol 9, Iss 3 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	meningococcus serogroup B Neisseria meningitidis factor H binding protein vaccines Microbiology QR1-502
spellingShingle	meningococcus serogroup B Neisseria meningitidis factor H binding protein vaccines Microbiology QR1-502 Jan Poolman Toward an Improved Meningococcal Serogroup B Assay
description	ABSTRACT Because of diverse sequences and differential expression of surface structures on individual invasive Neisseria meningitidis serogroup B (MenB) strains, predicting the efficacy of MenB vaccines using traditional human serum bactericidal assays (hSBA) is impractical. The meningococcal antigen surface expression (MEASURE) assay uses flow cytometry to quantitate the expression of factor H binding proteins (fHbp) contained in the bivalent rLP2086 MenB vaccine. To date, experience with MEASURE has been lacking, and in a long-awaited article, McNeil et al. (mBio 9:e00036-18, https://doi.org/10.1128/mBio.00036-18), provide detailed mapping of a cross-reactive antibody binding epitope and explore the potential utility of MEASURE in predicting the susceptibility of individual MenB strains to antibody-mediated killing. Remaining questions center around why some strains with high fHbp expression are nonsusceptible to anti-fHbp antibody killing. Consideration of alternative methods, such as a standardized enzyme-linked immunosorbent assay (ELISA), might offer a more readily available and reproducible assay for wider use.
format	article
author	Jan Poolman
author_facet	Jan Poolman
author_sort	Jan Poolman
title	Toward an Improved Meningococcal Serogroup B Assay
title_short	Toward an Improved Meningococcal Serogroup B Assay
title_full	Toward an Improved Meningococcal Serogroup B Assay
title_fullStr	Toward an Improved Meningococcal Serogroup B Assay
title_full_unstemmed	Toward an Improved Meningococcal Serogroup B Assay
title_sort	toward an improved meningococcal serogroup b assay
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/40246ba6b9ad40deadc77a30f334797e
work_keys_str_mv	AT janpoolman towardanimprovedmeningococcalserogroupbassay
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Toward an Improved Meningococcal Serogroup B Assay

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