The Papain-like Cysteine Protease <i>HpXBCP3</i> from <i>Haematococcus pluvialis</i> Involved in the Regulation of Growth, Salt Stress Tolerance and Chlorophyll Synthesis in Microalgae

The Papain-like Cysteine Protease <i>HpXBCP3</i> from <i>Haematococcus pluvialis</i> Involved in the Regulation of Growth, Salt Stress Tolerance and Chlorophyll Synthesis in Microalgae

The papain-like cysteine proteases (PLCPs), the most important group of cysteine proteases, have been reported to participate in the regulation of growth, senescence, and abiotic stresses in plants. However, the functions of PLCPs and their roles in stress response in microalgae was rarely reported....

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Autores principales: Wenfu Liu, Chunli Guo, Danqiong Huang, Hui Li, Chaogang Wang
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
<i>Haematococcus pluvialis</i>
<i>HpXBCP3</i>
<i>Chlamydomonas reinhardtii</i>
salt stress sensitivity
chlorophyll synthesis
cell proliferation
Biology (General)
QH301-705.5
Chemistry
QD1-999
Acceso en línea:https://doaj.org/article/402ac037b2ae439c87078c9a41438923
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spelling oai:doaj.org-article:402ac037b2ae439c87078c9a414389232021-11-11T17:00:21ZThe Papain-like Cysteine Protease <i>HpXBCP3</i> from <i>Haematococcus pluvialis</i> Involved in the Regulation of Growth, Salt Stress Tolerance and Chlorophyll Synthesis in Microalgae10.3390/ijms2221115391422-00671661-6596https://doaj.org/article/402ac037b2ae439c87078c9a414389232021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11539https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The papain-like cysteine proteases (PLCPs), the most important group of cysteine proteases, have been reported to participate in the regulation of growth, senescence, and abiotic stresses in plants. However, the functions of PLCPs and their roles in stress response in microalgae was rarely reported. The responses to different abiotic stresses in <i>Haematococcus pluvialis</i> were often observed, including growth regulation and astaxanthin accumulation. In this study, the cDNA of <i>HpXBCP3</i> containing 1515 bp open reading frame (ORF) was firstly cloned from <i>H. pluvialis</i> by RT-PCR. The analysis of protein domains and molecular evolution showed that <i>HpXBCP3</i> was closely related to <i>AtXBCP3</i> from <i>Arabidopsis</i>. The expression pattern analysis revealed that it significantly responds to NaCl stress in <i>H</i><i>. pluvialis</i>. Subsequently, transformants expressing <i>HpXBCP3</i> in <i>Chlamydomonas reinhardtii</i> were obtained and subjected to transcriptomic analysis. Results showed that <i>HpXBCP3</i> might affect the cell cycle regulation and DNA replication in transgenic <i>Chlamydomonas</i>, resulting in abnormal growth of transformants. Moreover, the expression of <i>HpXBCP3</i> might increase the sensitivity to NaCl stress by regulating ubiquitin and the expression of WD40 proteins in microalgae. Furthermore, the expression of <i>HpXBCP3</i> might improve chlorophyll content by up-regulating the expression of NADH-dependent glutamate synthases in <i>C. reinhardtii</i>. This study indicated for the first time that <i>HpXBCP3</i> was involved in the regulation of cell growth, salt stress response, and chlorophyll synthesis in microalgae. Results in this study might enrich the understanding of PLCPs in microalgae and provide a novel perspective for studying the mechanism of environmental stress responses in <i>H. pluvialis</i>.Wenfu LiuChunli GuoDanqiong HuangHui LiChaogang WangMDPI AGarticle<i>Haematococcus pluvialis</i><i>HpXBCP3</i><i>Chlamydomonas reinhardtii</i>salt stress sensitivitychlorophyll synthesiscell proliferationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11539, p 11539 (2021)
institution DOAJ
collection DOAJ
language EN
topic <i>Haematococcus pluvialis</i>
<i>HpXBCP3</i>
<i>Chlamydomonas reinhardtii</i>
salt stress sensitivity
chlorophyll synthesis
cell proliferation
Biology (General)
QH301-705.5
Chemistry
QD1-999
spellingShingle <i>Haematococcus pluvialis</i>
<i>HpXBCP3</i>
<i>Chlamydomonas reinhardtii</i>
salt stress sensitivity
chlorophyll synthesis
cell proliferation
Biology (General)
QH301-705.5
Chemistry
QD1-999
Wenfu Liu
Chunli Guo
Danqiong Huang
Hui Li
Chaogang Wang
The Papain-like Cysteine Protease <i>HpXBCP3</i> from <i>Haematococcus pluvialis</i> Involved in the Regulation of Growth, Salt Stress Tolerance and Chlorophyll Synthesis in Microalgae
description The papain-like cysteine proteases (PLCPs), the most important group of cysteine proteases, have been reported to participate in the regulation of growth, senescence, and abiotic stresses in plants. However, the functions of PLCPs and their roles in stress response in microalgae was rarely reported. The responses to different abiotic stresses in <i>Haematococcus pluvialis</i> were often observed, including growth regulation and astaxanthin accumulation. In this study, the cDNA of <i>HpXBCP3</i> containing 1515 bp open reading frame (ORF) was firstly cloned from <i>H. pluvialis</i> by RT-PCR. The analysis of protein domains and molecular evolution showed that <i>HpXBCP3</i> was closely related to <i>AtXBCP3</i> from <i>Arabidopsis</i>. The expression pattern analysis revealed that it significantly responds to NaCl stress in <i>H</i><i>. pluvialis</i>. Subsequently, transformants expressing <i>HpXBCP3</i> in <i>Chlamydomonas reinhardtii</i> were obtained and subjected to transcriptomic analysis. Results showed that <i>HpXBCP3</i> might affect the cell cycle regulation and DNA replication in transgenic <i>Chlamydomonas</i>, resulting in abnormal growth of transformants. Moreover, the expression of <i>HpXBCP3</i> might increase the sensitivity to NaCl stress by regulating ubiquitin and the expression of WD40 proteins in microalgae. Furthermore, the expression of <i>HpXBCP3</i> might improve chlorophyll content by up-regulating the expression of NADH-dependent glutamate synthases in <i>C. reinhardtii</i>. This study indicated for the first time that <i>HpXBCP3</i> was involved in the regulation of cell growth, salt stress response, and chlorophyll synthesis in microalgae. Results in this study might enrich the understanding of PLCPs in microalgae and provide a novel perspective for studying the mechanism of environmental stress responses in <i>H. pluvialis</i>.
format article
author Wenfu Liu
Chunli Guo
Danqiong Huang
Hui Li
Chaogang Wang
author_facet Wenfu Liu
Chunli Guo
Danqiong Huang
Hui Li
Chaogang Wang
author_sort Wenfu Liu
title The Papain-like Cysteine Protease <i>HpXBCP3</i> from <i>Haematococcus pluvialis</i> Involved in the Regulation of Growth, Salt Stress Tolerance and Chlorophyll Synthesis in Microalgae
title_short The Papain-like Cysteine Protease <i>HpXBCP3</i> from <i>Haematococcus pluvialis</i> Involved in the Regulation of Growth, Salt Stress Tolerance and Chlorophyll Synthesis in Microalgae
title_full The Papain-like Cysteine Protease <i>HpXBCP3</i> from <i>Haematococcus pluvialis</i> Involved in the Regulation of Growth, Salt Stress Tolerance and Chlorophyll Synthesis in Microalgae
title_fullStr The Papain-like Cysteine Protease <i>HpXBCP3</i> from <i>Haematococcus pluvialis</i> Involved in the Regulation of Growth, Salt Stress Tolerance and Chlorophyll Synthesis in Microalgae
title_full_unstemmed The Papain-like Cysteine Protease <i>HpXBCP3</i> from <i>Haematococcus pluvialis</i> Involved in the Regulation of Growth, Salt Stress Tolerance and Chlorophyll Synthesis in Microalgae
title_sort papain-like cysteine protease <i>hpxbcp3</i> from <i>haematococcus pluvialis</i> involved in the regulation of growth, salt stress tolerance and chlorophyll synthesis in microalgae
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/402ac037b2ae439c87078c9a41438923
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