Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk
Raw cow milk is considered one of the most important vehicles for pathogenic bacteria like Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes. These three bacteria are responsible for foodborne diseases. Routine microbiological methods to detect these microorganisms in cow mil...
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| Formato: | article |
| Lenguaje: | EN ES |
| Publicado: |
Pontificia Universidad Javeriana
2019
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/402e5767a81d43f6bca21d4ebe8ae029 |
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| Sumario: | Raw cow milk is considered one of the most important vehicles for
pathogenic bacteria like Salmonella spp., Escherichia coli O157:H7, and
Listeria monocytogenes. These three bacteria are responsible for foodborne
diseases. Routine microbiological methods to detect these microorganisms in
cow milk can be complicated and time consuming. The aim of this work was
to evaluate a method to simultaneously detect Salmonella spp., Escherichia coli
O157:H7, and Listeria monocytogenes in experimentally contaminated cow
milk. The assessed method combined a standard microbiological culture
step, using a pre-enrichment medium that favors the growth of the
three focal microorganisms: SEL broth, followed by a single PCR assay.
A total of 43 interference bacterial strains were used to evaluate the
method’s specificity. The detection rate for the microbiological method
with standard culture media was 10 UFC/mL, and that of the PCR
detection, following pre-enrichment in SEL broth, was 10 UFC/mL for
S. enterica and L. monocytogenes and between 1 and 5 UFC/mL for E. coli
O157:H7. The PCR method showed specificity for the reference strains.
Simultaneous detection by multiple PCR using SEL broth was successful for
the detection of S. enterica, E. coli O157:H7, and L. monocytogenes in samples
of experimentally contaminated cow milk, featuring both a high detection
rate and a high specificity. This approach promises to be a feasible routine
procedure when testing milk samples in industry and public health control
setups. |
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